Abstract
In this study 28 bacterial strains, isolated from greenwaters-polluted-soil, were investigated for their ability to grow in presence of phenols added to Mineral Basal Medium (MBM) in aerobic conditions. In particular, three of them were found to be able to use as sole carbon source phenol, cathecol, caffeic acid and ferulic acid with efficiency ranging from 76% (phenol in 5 days, millimolar concentration from 3.7 10−2 to 9 10−3) to 95% (ferulic acid in 2 days millimolar concentration from 6.8 10−1 to 3 10−2). For these strains the taxonomic position was studied by amplification and sequencing of 16S rRNA genes. The isolated strains were classified belonging to Arthrobacter sulfureus, Pseudomonas synxantha and Pseudomonas oryzihabitans. Noteworthy, for the first time such Pseudomonas strains have been shown to be able to use polyphenols as the only carbon source in vitro. In fact, to the best of our knowledge, this kind of study were not done on Ps. Synxantha, while it was recently shown the ability of P. oryzihabitans to degrade catechol. These findings may open to new biotechnological applications for the degradation of polyphenols.
Similar content being viewed by others
References
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleide Acid Res 25:3389–3402
Amer RA (2008) Newly isolated Pandoraea sp. capable on phenol degradation. Res J Microbiol 3(10):622–629
Ammar E, Nasri M, Medhioub K (2005) Isolation of Enterobacteria able to degrade simple aromatic compounds from the wastewater from olive oil extraction. World J Microbiol Biotech 21:253–259
Anzai Y, Kim H, Park JY, Wakabayashi H, Oyaizu H (2000) Phylogenetic affiliation of the pseudomonas based on 16S rRNA sequence. Int J Syst Evol Microbiol 50(4):1563–1589
Aresta M, Dileo C, Narracci M, Tommasi I (2001) Anaerobic biodegradation of phenols. Environmental Chemistry. In Lichtfouse E (ed.), ACE, p 201
Aresta M, Dibenedetto A, Narracci M, Tommasi I (2003) A technology for the treatment of olive-mill waste water in a continuously fed plant. An insight into the degradation mechanism of methoxy-polyphenols. Env Chem Lett 1:13–18
Arutchelvan V, Kanakasabai V, Elangovan R, Nagarajan S (2004) Physicochemical characteristics of greenwaters from bakelite manufacturing industry. Indian J Environ Ecoplann 8:757–760
Borja R, Alba J, Banks C (1997) Impact of the main phenolic compounds of greenwaters (OMW) on the kinetics of acetoclastic methanogenesis. Proc Biochem 32(2):121–133
Di Gioia D, Barberio C, Spagnesi S, Marchetti L, Fava F (2002) Characterization of four olive-mill-wastewater indigenous bacterial strains capable of aerobically degrading hydroxylated and methoxylated monocyclic aromatic compounds. Arch Microbiol 178:208–217
Dibenedetto A, Lo Noce RM, Narracci M, Aresta M (2006) Correlation structure-biodegradation of polyphenols by Thauera aromatica K172 in anaerobic conditions. Chem Ecol 22(1):133–143
El-Sayed WS, Ibrahim MK, Abu-Shady M, El-Beih F, Ohmura N, Saiki H, Ando A (2003) Isolation and characterization of phenol-catabolizing bacteria from a coking plant. Biosc Biotech Biochem 67:2026–2029
Ercolini D, Russo F, Blaiotta G, Pep O, Mauriello G, Villani F (2007) Simultaneous detection of Pseudomonas fragi, P. lundensis, and P. putida from meat by use of a multiplex pcr assay targeting the carA Gene. Appl Environ Microb 73(7):2354–2359
Fadil K, Chahlaoui A, Ouahbi A, Zaid A, Borja R (2003) Aerobic biodegradation and detoxification of greenwaters from the olive oil industry. Int Biodet Biodeg 51(1):37–41
Gazzetta Ufficiale Italiana, 1996 n° 265
Ghosh S, Sachan A, Mitra A (2006) Formation of vanillic acid from ferulic acid by Paecilomyces variotii MTCC 6581. Cur Sci 90(6):825–829
Grit N, Riho T, Liis M, Maia K, Frieder S, Hermann JH (2004) Simultaneous degradation of atrazine and phenol by Pseudomonas sp. strain ADP: effects of toxicity and adaptation. Appl Environ Microbiol 70:1907–1912
ISMEA (2004) L’olivicoltura italiana nella campagna 2003/04. Quaderni di filiera, 12
Janssen PH, Yates PS, Grinton BE, Taylor PM, Sait M (2002) Improved Culturability of Soil Bacteria and Isolation in Pure Culture of Novel Members of the Divisions Acidobacteria, Actinobacteria, Proteobacteria, and Verrucomicrobia. Appl Environ Microbiol 68(5):2391–2396
Jussila MM, Zhao J, Suominen L, Lindstrom K (2007) TOL plasmid transfer during bacterial conjugation in vitro and rizhoremediation of oil compounds in vivo. Env Poll 146:510–524
Klijn N, Weerkamp AH, de Vos WM (1995) Detection and characterization of lactose-utilizing Lactococcus subsp. in natural ecosystems. Appl Environm Microbiol 61:788–792
Kumar A, Kumar S, Kumar S (2005) Biodegradation kinetics of phenol and catechol using Pseudomonas putida MTCC 1194. Biochem Eng J 22(2):151–159
Michaluart P, Masferrer JL, Carothers AM, Subbaramaiah K, Zweifel BS, Koboldt C, Mestre JR, Grunberger D, Sacks PG, Tanabe T, Dannenberg AJ (1999) Inhibitory effect of caffeic acid phenethyl ester on the activity and expression of cyclooxygenase-2 in human oral epithelial cell and in rat model of inflammation. Cancer Res 59:2347–2352
Nielsen SE, Sandström B (2003) Simultaneous determination of hydroxycinnamates and catechins in human urine samples by column switching liquid chromatography coupled to atmospheric pressure chemical ionization mass spectrometry. J Chromat B 787:369–379
Othmer K (1995) Kirk-othmer encyclopedia of chemical technology. Wiley-Interscience, New York
Peters M, Heinaru E, Talpsep H, Ward H, Stottmeister U, Heinaru A, Nurk A (1997) Acquisition of a deliberately introduced phenol degradation operon, pheAB, by different indigenous Pseudomonas species. Appl Environ Microbiol 63:4899–4906
Priefert H, Rabenhorst J, Steinbüchel A (2001) Biotechnological production of vanillin. Appl Microbiol Biotechnol 56:296–314
Purohit HJ, Raje DV, Kapley A (2003) Identification of signature and primers specific to genus Pseudomonas using mismatched patterns of 16SrDNA sequences. BMC Bioinformatics, 4:19 URL http//www.biomedcentral.com/1471-2105/4/19
Reinders RD, Biesterveld S, Bijker PG (2001) Survival of Escherichia coli O157:H7 ATCC 43895 in a model apple juice medium with different concentrations of proline and caffeic acid. Appl Environ Microbiol 67:2863–2866
Rosazza JP, Huang Z, Dostal L, Volm T, Rousseau B (1995) Biocatalytic transformations of ferulic acid: an aboundant aromatic natural product. J Ind Microbiol 15:457–471
Shen H, Wang Y (1995) Simultaneous Chromium Reduction and Phenol Degradation in a Cocolture of Escherichia coli ATCC 33456 and Pseudomonas putida DMP-1. Appl Environ Microbiol 61:2754–2758
Shingler V (1996) Molecular and regulatory check points in phenol degradation by Pseudomonas sp. CF600. In: Nakazawa T, Furukawa K, Haas D, Silver S (eds) Molecular biology of pseudomonas. American Society for Microbiology, Washington, pp 153–164
Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599
Tayeb LA, Ageron E, Grimont F, Grimont PAD (2005) Molecular phylogeny of the genus Pseudomonas based on rpoB sequences and application for the identification of isolates. Res Microbiol 156(5–6):763–773
Vijayagopal V, Viruthagiri V (2005) Butch kinetic studies in phenol biodegradation and comparison. Ind J Biotech 4:565–567
Zharkikh A, Li WH (1995) Estimation of confidence in phylogeny: the complete-and-partial bootstrap technique. Mol Phylogenet Evol 4:44–63
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Aresta, M., Acquaviva, M.I., Baruzzi, F. et al. Isolation and characterization of polyphenols-degrading bacteria from olive-mill wastewaters polluted soil. World J Microbiol Biotechnol 26, 639–647 (2010). https://doi.org/10.1007/s11274-009-0217-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11274-009-0217-x