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Physiological and molecular characterisation of Saccharomyces cerevisiae cachaça strains isolated from different geographic regions in Brazil

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Abstract

In this work, 74 Saccharomyces cerevisiae strains isolated from cachaça fermentation of six different geographic regions in Brazil were characterized by mitochondrial DNA restriction fragment length polymorphism (mtDNA-RFLP) and by their ability to grow on stress conditions occurring during the cachaça fermentation process. Cachaça S. cerevisiae strains showed high mtDNA-RFLP polymorphism with the occurrence of 32 different molecular patterns. The S. cerevisiae strains presenting prevalent mtDNA were able to grow better in the stress conditions than strains represented by infrequent patterns. The principal coordinate analysis on 17 stress conditions revealed that the major source of growth variation were high ethanol concentrations and low temperatures. These results indicate that the stress conditions occurring in the fermentation process influence the prevalence of the most adapted S. cerevisiae strains in each distillery. The physiological tests used in our study can be used as a criterion for rapidly selecting autochthonous yeast strains for further purposes such as the selection of fermentative starters of S. cerevisiae strains.

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References

  • Araújo RAC, Gomes FCO, Moreira ESA, Cisalpino PS, Rosa CA (2007) Monitoring Saccharomyces cerevisiae populations by mtDNA restriction analysis and other molecular typing methods during spontaneous fermentation for production of the artisanal cachaça. Braz J Microbiol 38:217–223. doi:10.1590/S1517-83822007000200006

    Article  Google Scholar 

  • Belloch C, Orlic S, Barrio E, Querol A (2008) Fermentative stress adaptation of hybrids within the Saccharomyces sensu stricto complex. Int J Food Microbiol 122:188–195. doi:10.1016/j.ijfoodmicro.2007.11.083

    Article  CAS  Google Scholar 

  • Brown SW, Oliver SG, Harrison DEF, Righelato RC (1981) Ethanol inhibition of yeast grown and fermentation: differences in the magnitude and complexity of the effect. Eur J Appl Microbiol Biotechnol 11:151–155

    Article  CAS  Google Scholar 

  • Carrasco P, Querol A, del Olmo M (2001) Analysis of stress resistance of commercial wine yeast strains. Arch Microbiol 175:450–457. doi:10.1007/s002030100289

    Article  CAS  Google Scholar 

  • de Llanos R, Fernández-Espinar MT, Querol A (2006) A comparison of clinical and food Saccharomyces cerevisiae isolates on the basis of potential virulence factors. Antonie van Leeuwenhoek 90:221–231. doi:10.1007/s10482-006-9077-7

    Article  CAS  Google Scholar 

  • Garay-Arroyo A, Covarrubias AA, Clark I, Niño I, Gosset G, Martinez A (2004) Response to different environmental stress conditions of industrial and laboratory Saccharomyces cerevisiae strains. Appl Microbiol Biotechnol 63:734–741. doi:10.1007/s00253-003-1414-4

    Article  CAS  Google Scholar 

  • Gomes FCO, Silva CLC, Marini MM, Oliveira ES, Rosa CA (2007) Use of selected indigenous Saccharomyces cerevisiae strains for the production of the traditional cachaça in Brazil. J Appl Microbiol 103:2438–2447. doi:10.1111/j.1365-2672.2007.03486.x

    Article  CAS  Google Scholar 

  • Guerra JB, Araújo RAC, Pataro C, Franco GR, Moreira ESA, Mendonça-Hagler LC, Rosa CA (2001) Genetic diversity of Saccharomyces cerevisiae strains during the 24 h fermentative cycle for the production of the artisanal Brazilian cachaça. Lett Appl Microbiol 33:106–111. doi:10.1046/j.1472-765x.2001.00959.x

    Article  CAS  Google Scholar 

  • Guillamón JM, Barrio E, Querol A (1996) Characterization of wine yeast strains of the Saccharomyces genus on the basis of molecular markers: relationship between distance and geographic or ecological origin. Syst Appl Microbiol 19:122–132

    Google Scholar 

  • Kishimoto M, Goto S (1995) Growth temperature and electrophoretic karyotype as tools for practical discrimination of S. bayanus and S. cerevisiae. J Gen Appl Microbiol 41:239–247

    Article  CAS  Google Scholar 

  • Kurtzman CP, Fell JW (1998) The yeasts, a taxonomic study. Elsevier, Amsterdam

    Google Scholar 

  • Lafon-Lafourcade S (1983) Wine and brandy. In: Rehm HJ, Reed G (eds) Biotechnology, vol. 5. Food and feed production with microorganisms. Verlag Chemie, Weinheim, pp 81–163

  • Larue F, Lafon-Lafourcade S, Ribéreau-Gayon P (1980) Relationship between the sterol content of yeast cells and their fermentation activity in grape must. Appl Environ Microbiol 66:4456–4461

    Google Scholar 

  • Martini A, Vaughan-Martini A (1990) Grape must fermentation: past and present. In: Spencer JFT, Spencer M (eds) Yeast technology. Springer, Germany, pp 105–123

    Google Scholar 

  • Molina AM, Swiegers JH, Varela C, Pretorius IS, Agosin E (2007) Influence of wine fermentation temperature on the synthesis of yeast-derived volatile aroma compounds. Appl Microbiol Biotechnol 77:675–687. doi:10.1007/s00253-007-1194-3

    Article  CAS  Google Scholar 

  • Morais PB, Rosa CA, Linardi VR, Pataro C, Maia ABRA (1997) Characterization and succession of yeast populations associated with spontaneous fermentations during the production of Brazilian sugar-cane aguardente. World J Microbiol Biotechnol 13:241–243

    Article  Google Scholar 

  • Nadal D, Colomer B, Pina B (1996) Molecular polymorphism distribution in phenotypically distinct populations of wine yeast strains. Appl Environ Microbiol 62:1944–1950

    CAS  Google Scholar 

  • Naumov GI (1996) Genetic identification of biological species in the Saccharomyces sensu stricto complex. J Ind Microbiol 17:295–302

    Article  CAS  Google Scholar 

  • Ogawa Y, Nitta A, Uchiyama H, Imamura T, Shimoi H, Ito K (2000) Tolerance mechanism of the ethanol tolerant mutant of sake yeast. J Biosci Bioeng 90:313–320. doi:10.1016/S1389-1723(00)80087-0

    CAS  Google Scholar 

  • Pataro C, Santos A, Corrêa SR, Morais PB, Linardi VR, Rosa CA (1998) Physiological characterization of yeasts isolated from artisanal fermentation in an aguardente distillery. Rev. Microbiol. 29:104–108

    CAS  Google Scholar 

  • Pataro C, Guerra JB, Petrillo-Peixoto ML, Mendonça-Hagler LC, Linardi VR, Rosa CA (2000) Yeast communities and genetic polymorphism of Saccharomyces cerevisiae strains associated with artisanal fermentation in Brazil. J Appl Microbiol 88:1–9. doi:10.1046/j.1365-2672.2000.01092.x

    Article  Google Scholar 

  • Pataro C, Guerra JB, Neves MJ, Pimentel PF, Rosa CA (2002) Trehalose accumulation, invertase activity and physiological characteristics of yeasts isolated from 24 h fermentative cycles during the artisanal Brazilian cachaça. Braz J Microbiol 33:202–208. doi:10.1590/S1517-83822002000300003

    Article  CAS  Google Scholar 

  • Pretorius IS (2000) Tailoring wine yeast for the new millennium: novel approaches to the ancient art of winemaking. Yeast 16:675–729. doi:10.1002/1097-006120000615

    Article  CAS  Google Scholar 

  • Querol A, Barrio E, Huerta T, Ramón D (1992) Molecular monitoring of wine fermentations conducted by active dry yeasts strains. Appl Environ Microbiol 58:2948–2953

    CAS  Google Scholar 

  • Querol A, Barrio E, Ramón D (1994) Population dynamics of natural Saccharomyces strains during wine fermentation. Int J Food Microbiol 21:315–323. doi:10.1016/0168-1605(94)90061-2

    Article  CAS  Google Scholar 

  • Rosa CA, Soares AM, Faria JB (2009) Cachaça production. In: Ingledew WM (ed) The alcohol textbook, 5th edn. Nottingham University, Nottingham

    Google Scholar 

  • Schwan RF, Mendonça AT, Silva JJ Jr, Rodrigues V, Wheals AE (2001) Microbiology and physiology of cachaça fermentations. Antonie van Leeuwenhoek 79:89–96. doi:10.1023/A:1010225117654

    Article  CAS  Google Scholar 

  • Vianna CR, Silva CLC, Neves MJ, Rosa CA (2008) Saccharomyces cerevisiae strains from traditional fermentations of Brazilian cachaça: trehalose metabolism, heat and ethanol resistance. Antonie van Leeuwenhoek 93:205–217

    Article  CAS  Google Scholar 

  • Yarrow D (1998) Methods for the isolation, maintenance and identification of yeasts. In: Kurtzman CP, Fell JW (eds) The yeasts a taxonomic study. Elsevier, Amsterdam, pp 77–100

    Chapter  Google Scholar 

  • Zuzuarregui A, del Olmo M (2004) Analyses of stress resistance under laboratory conditions constitute a suitable criterion for wine yeast selection. Antonie van Leeuwenhoek 85:271–280. doi:10.1023/B:ANTO.0000020162.21248.53

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by CICYT grants AGL2006-12703-CO2-01 and AGL2007-65498-C02-02 from the Spanish Ministry of Science and Technology. We also thank to Programme ALBAN, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do estado de Minas Gerais (FAPEMIG) for finnancial support.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Departamento de Microbiologia, ICB, C. P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil

    Fernanda Badotti & Carlos A. Rosa

  2. Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universidad de Valencia, Polígono La Coma s/n, 46980, Paterna, Spain

    Fernanda Badotti & Eladio Barrio

  3. Instituto de Agroquímica y Tecnología de los Alimentos, CSIC, P.O. Box 73, Apartado de Correos 73, 46100, Burjassot, Valencia, Spain

    Carmela Belloch & Amparo Querol

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  1. Fernanda Badotti
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  2. Carmela Belloch
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  3. Carlos A. Rosa
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  4. Eladio Barrio
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  5. Amparo Querol
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Correspondence to Amparo Querol.

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Badotti, F., Belloch, C., Rosa, C.A. et al. Physiological and molecular characterisation of Saccharomyces cerevisiae cachaça strains isolated from different geographic regions in Brazil. World J Microbiol Biotechnol 26, 579–587 (2010). https://doi.org/10.1007/s11274-009-0206-0

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