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Cohabitation within mice of Salmonella typhimurium seqA mutant increases its virulence

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Abstract

The efficacy of a vaccine is conditioned by its capacity to elicit a protective immune response. The principal safety concerns of live vaccine are virulence reversion. The aim of this work was to evaluate the virulence of Salmonella typhimurium seqA mutant after cohabitation with mice. Our results indicated that LD50 of hosted strains were at least twofold lower than those of parental strains. Also, the in vivo competition assays have showed that the development of a systemic infection was most obvious for recovered strains than for control strains. In addition, the number of hosted mutants colonizing spleen and liver was relatively higher than control strains. Adhesion and invasion experiments were performed in order to compare the pathogenicity of Salmonella. For instance recovered-mutant attached to epithelial cells (KB cells) better than parental strains. According to these results, we report that in vivo adaptation of Salmonella typhimurium seqA mutants can increase their virulence.

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Acknowledgments

This work was supported by: «Ministère de l’Enseignement Supérieur; Faculté des Sciences de Bizerte-Département de Biologie». We thank Mr. Laszlo N. Csonka (Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA) for providing wild-type strain LT2 and TL3283 (seqA101::MudJ).

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Authors and Affiliations

  1. Département des Sciences de la vie, Unité de Biochimie des Lipides et interaction des macromolécules en Biologie, 03/UR/0902, Faculté des Sciences de Bizerte, Laboratoire de Biochimie et Biologie Moléculaire, Zarzouna, 7021, Tunisia

    Messaoudi Nadia, Chatti Abdelwaheb & Landoulsi Ahmed

  2. Centre des Recherches et des Technologies des Eaux (CERTE), Laboratoire de Traitement et Recyclage des Eaux, Technozpole Borj Cédria, BP 273, Soliman, 8020, Tunisia

    Chatti Abdelwaheb

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  1. Messaoudi Nadia
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  2. Chatti Abdelwaheb
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  3. Landoulsi Ahmed
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Correspondence to Chatti Abdelwaheb.

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Nadia, M., Abdelwaheb, C. & Ahmed, L. Cohabitation within mice of Salmonella typhimurium seqA mutant increases its virulence. World J Microbiol Biotechnol 26, 183–187 (2010). https://doi.org/10.1007/s11274-009-0141-0

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  • DOI: https://doi.org/10.1007/s11274-009-0141-0

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