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Management of palm oil mill effluent through production of cellulases by filamentous fungi

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Abstract

A laboratory scale study to evaluate the potentiality of filamentous fungi for the production of cellulolytic enzymes using palm oil mill effluent (POME) as a basal medium was initiated. A total of 25 filamentous fungi in which 16 filamentous fungi were isolated and purified from oil palm industrial residues and 9 strains from laboratory stock were screened using POME with 1% total suspended solids. Trichoderma reesei RUT C-30 was identified as a potential strain for cellulolytic enzyme production as compared to other genera of Aspergillus, Penicillum, Rhizopus, Phanerochaete, Trichoderma and basidiomycete groups. The results showed that T. reesei RUT C-30 gave the highest filter paper cellulase and carboxy methyl cellulase activity of 0.917 and 2.51 U/ml respectively at day 5 of fermentation. Other parameters such as growth formation, pH, filterability and total biosolids were observed to evaluate the bioconversion process.

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Acknowledgments

The authors thank to the Ministry of Higher Education (MOHE) for approval a research grant under Fundamental Research Grant Scheme (FRGS 0207-49). The supply of experimental samples by the Seri Ulu Langat Palm Oil Mill Sdn Bhd and West Oil Mill, Sime Darby Plantation, is acknowledged.

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Authors and Affiliations

  1. Bioenvironmental Engineering Research Unit (BERU), Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia (IIUM), 50728, Kuala Lumpur, Gombak, Malaysia

    Shah Samiur Rashid, Md. Zahangir Alam, M. Ismail A. Karim & M. Hamzah Salleh

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  1. Shah Samiur Rashid
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  2. Md. Zahangir Alam
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  3. M. Ismail A. Karim
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  4. M. Hamzah Salleh
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Correspondence to Md. Zahangir Alam.

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Rashid, S.S., Alam, M.Z., Karim, M.I.A. et al. Management of palm oil mill effluent through production of cellulases by filamentous fungi. World J Microbiol Biotechnol 25, 2219–2226 (2009). https://doi.org/10.1007/s11274-009-0129-9

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  • DOI: https://doi.org/10.1007/s11274-009-0129-9

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