World Journal of Microbiology and Biotechnology

, Volume 25, Issue 12, pp 2083–2094 | Cite as

Approaches for refining heterologous protein production in filamentous fungi

  • Ruchika Sharma
  • Meenu KatochEmail author
  • P. S. Srivastava
  • G. N. Qazi


Fungi combine the advantages of a microbial system such as a simple fermentability with the capability of secreting proteins that are modified according to a general eukaryotic scheme. Filamentous fungi such as Aspergillus niger efficiently secrete genuine proteins but the secretion of recombinant proteins turned out be a difficult task. Aspergillus niger is an attractive organism because of its high secretion capacity and is frequently used as a model organism. Whereas high production yields can be obtained when homologous proteins are expressed, much lower amounts are obtained with the production of heterologous proteins. To fully exploit the potential of filamentous fungi, understanding of the molecular genetics, their physiology, and the glycosylation metabolism has to be investigated and clarified in more detail. This review summarizes recent developments in heterologous protein production by filamentous fungi and also generalizes the possibilities of improving the protein production by various genetic and bioprocessing approaches, thereby easing recognition of filamentous fungi as a relevant and reliable expression platform.


Filamentous fungi Heterologous proteins Expression system 


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Ruchika Sharma
    • 1
  • Meenu Katoch
    • 1
    Email author
  • P. S. Srivastava
    • 2
  • G. N. Qazi
    • 1
  1. 1.Biotechnology DivisionIndian Institute of Integrative Medicine (CSIR)JammuIndia
  2. 2.Department of BiotechnologyJamia HamdardNew DelhiIndia

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