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Morphological changes of Klebsiella pneumoniae in response to Cefotaxime: a scanning electron microscope study

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Abstract

Multi drug resistant Klebsiella pneumoniae showed stepwise adaptation when grown in increasing concentration of Cefotaxime eventually reaching a maximum of 2 mg/ml. The resultant Cefotaxime resistant mutant strain was stable and did not revert to susceptibility on frequent subculturing. The response of the cells to different concentration of Cefotaxime was examined by scanning electron microscope which showed that the size of the bacterium increased with increasing concentration of Cefotaxime.

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References

  • Aldsworth TG, Sharman RL, Dodd CER (1999) Bacterial suicide through stress. Cell Mol Life Sci 56:378–383. doi:10.1007/s000180050439

    Article  CAS  Google Scholar 

  • Dodd CER, Sharman RL, Bloomfied SF, Booth IR, Stewaet GSAB (1999) Inimical processes: bacterial self-distraction and sublethal injury. Trends Food Sci Technol 8:238–241. doi:10.1016/S0924-2244(97)01043-1

    Article  Google Scholar 

  • Heipieper HJ, Weber FJ, Sikkema J, Keweloh H, de Bont JAM (1993) Mechanism behind resistance of whole cells to toxic organic solvents. Trends Biotechnol 12:409–415. doi:10.1016/0167-7799(94)90029-9

    Article  Google Scholar 

  • Kessel RG, Shib CY (1974) Scanning electron microscopy in biology. Spring-Verlag, Berlin, Heidelberg, New York

    Google Scholar 

  • Neumann G, Veerangouda Y, Karegoudar TB, Sahin O, Mausezahl I, Kabelitz N, Kappelmeyer U, Heipieper HJ (2005) Cells of pseudomonas putida and Enterobacter sp. adapt to toxic organic compounds by increasing their size. Extermophiles 9:163–168. doi:10.1007/s00792-005-0431-x

    Article  CAS  Google Scholar 

  • O’Hara GW, Glenn AR (1994) The adaptive acid tolerance response in root nodule bacteria and Escherichia coli. Arch Microbiol 161:286–292. doi:10.1007/BF00303582

    Article  Google Scholar 

  • Ritz M, Tholozan JL, Federighi M, Pilet MF (2001) Morphological and physiological characterization of Listeria monocytogenes subjected to high hydrostatic pressure. Appl Environ Microbiol 67:2240–2247. doi:10.1128/AEM.67.5.2240-2247.2001

    Article  CAS  Google Scholar 

  • Shi B, Xia X (2003) Morphological changes of pseudomonas pseudoalcaligenes in response to temperature selection. Curr Microbiol 46:120–123. doi:10.1007/s00284-002-3824-4

    Article  CAS  Google Scholar 

  • Thomas LV, Wimpenny JWT (1996) Investigation of the effect of combined variations in temperature, pH and NaCl concentrations on nisin inhibition of Listeria monocytogenes and Staphylococcus aureus. Appl Environ Microbiol 62:2006–2012

    CAS  Google Scholar 

  • Zanetti M, Gennaro R, Skerlavaj B et al (2002) Cathelicidin peptides as candidates for a novel class of antimicrobials. Curr Pharm Des 8:779–793. doi:10.2174/1381612023395457

    Article  CAS  Google Scholar 

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Acknowledgments

We would like to Thank Miss. Sushmita Potluri for her help in Scanning electron microscopy and Dr. P. M. Nimbargi, Department of microbiology, Gulbarga University, Gulbarga for his vocabulary and manuscript review and comments.

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Authors and Affiliations

  1. Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka, India

    H. Rajeshwari, S. Nagveni, Ajay Oli & Kelmani R. Chandrakanth

  2. National Institute of Virology, Pune, India

    Deepti Parashar

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  1. H. Rajeshwari
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  2. S. Nagveni
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  3. Ajay Oli
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  4. Deepti Parashar
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  5. Kelmani R. Chandrakanth
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Correspondence to Kelmani R. Chandrakanth.

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Rajeshwari, H., Nagveni, S., Oli, A. et al. Morphological changes of Klebsiella pneumoniae in response to Cefotaxime: a scanning electron microscope study. World J Microbiol Biotechnol 25, 2263–2266 (2009). https://doi.org/10.1007/s11274-009-0126-z

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  • DOI: https://doi.org/10.1007/s11274-009-0126-z

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