Abstract
This study has developed a novel multi-stage preculture strategy to control the morphology of filamentous Rhizopus oryzae in a 3.3-l stirred-tank reactor (STR), consequently enhancing the fumaric acid production. Using multi-stage precultures as inoculum, the morphology of R. oryzae was maintained as large clumps, small fluffy pellets, and entangled filaments. The highest fumaric acid production of 42.5 g/l with a yield of 55.6% was obtained in association with the small fluffy pellets which were formed in the third preculture at pH 3.0. The results indicated that the use of the multi-stage precultures as inoculum in the STR is a promising approach for commercial production of fumaric acid.
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Acknowledgments
This work was supported by grants from the Ministry of Science and Technology of China [National High Technology Research and Development Program of China (No. 2006AA02Z240), National Basic Research Program of China (No. 2007CB707805)] and the National Natural Science Foundation of China (Nos. 20576054 and 20706031).
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Fu, Y., Xu, Q., Li, S. et al. A novel multi-stage preculture strategy of Rhizopus oryzae ME-F12 for fumaric acid production in a stirred-tank reactor. World J Microbiol Biotechnol 25, 1871–1876 (2009). https://doi.org/10.1007/s11274-009-0076-5
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DOI: https://doi.org/10.1007/s11274-009-0076-5