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World Journal of Microbiology and Biotechnology

, Volume 25, Issue 9, pp 1615–1623 | Cite as

Degradation of hydrocarbons and biosurfactant production by Pseudomonas sp. strain LP1

  • Oluwafemi S. ObayoriEmail author
  • Matthew O. Ilori
  • Sunday A. Adebusoye
  • Ganiyu O. Oyetibo
  • Ayodele E. Omotayo
  • Olukayode O. Amund
Original Paper

Abstract

Pseudomonas sp. strain LP1, an organism isolated on the basis of its ability to grow on pyrene, was assayed for its degradative and biosurfactant production potentials when growing on crude, diesel and engine oils. The isolate exhibited specific growth rate and doubling time of 0.304 days−1 and 2.28 days, respectively on crude oil (Escravos Light). The corresponding values on diesel were 0.233 days−1 and 2.97 days, while on engine oil, were 0.122 days−1 and 5.71 days. The organism did not show significant biosurfactant production towards crude oil and diesel, but readily produced biosurfactant on engine oil. The highest Emulsification index (E24) value for the biosurfactant produced by LP1 on engine oil was 80.33 ± 1.20, on day 8 of incubation. Biosurfactant production was growth-associated. The surface-active compound which exhibited zero saline tolerance had its optimal activity at 50°C and pH 2.0.

Keywords

Biodegradation Biosurfactants Engine oil Hydrocarbons Pseudomonas 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Oluwafemi S. Obayori
    • 1
    Email author
  • Matthew O. Ilori
    • 1
  • Sunday A. Adebusoye
    • 1
  • Ganiyu O. Oyetibo
    • 1
  • Ayodele E. Omotayo
    • 1
  • Olukayode O. Amund
    • 1
  1. 1.Faculty of Science, Department of Botany and MicrobiologyUniversity of LagosAkoka, LagosNigeria

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