Abstract
The objective of this study was to maximize production of xylanase by a newly isolated strain Penicillium thiersii ZH-19. Response surface methodology was employed to study the effects of significant factors such as pH, temperature, xylan concentration, and cultivation time, on the production of xylanase by Penicillium thiersii ZH-19. The optimal fermentation parameters for enhanced xylanase production were found to be pH 7.72, temperature 24.8°C, xylan 13.2 g l−1 and the fermentation time 125.8 h. The model predicted a xylanase activity of 75.24 U ml−1. Verification of the optimization showed that the maximum xylanase production reached 73.50 U mL−1 in the flask experiments and 80.23 U mL−1 in the scale of 15-L fermenter under the optimal condition.
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References
Box GE, Behnken DW (1960) Some new three level designs for the study of quantitative variable. Technometrics 2:456–475. doi:10.2307/1266454
Chen C, Chen JL, Lin TY (1997) Purification and characterization of a xylanase from Trichoderma longibrachiatum for xylooligosaccharide production. Enzyme Microb Technol 21:91–96. doi:10.1016/S0141-0229(96)00236-0
Cui FJ, Li Y, Xu ZH, Sun K, Tao WY (2006) Optimization of the medium composition for production of mycelial biomass and exo-polymer by Grifola frondosa GF9801 using response surface methodology. Bioresour Technol 97:1209–1216. doi:10.1016/j.biortech.2005.05.005
Erland S, Henrion B, Martin F, Glover LA, Alexander IJ (1994) Identification of the ectomycorrhizal basidiomycete tylospora-fibrillosa donk by RFLP analysis of the PCR-amplified ITS and IGS regions of ribosomal DNA. New Phytol 126:525–532. doi:10.1111/j.1469-8137.1994.tb04251.x
Hang YD, Woodams EE (1997) Xylanolytic activity of commercial juice-processing enzyme preparations. Lett Appl Microbiol 24:389–392. doi:10.1046/j.1472-765X.1997.00144.x
Kuhad RC, Singh A (1993) Lignocellulose biotechnology: current and future prospects. Crit Rev Biotechnol 13:151–172. doi:10.3109/07388559309040630
Li Y, Lu J, Gu GX, Mao Z (2005) Characterization of the enzymatic degradation of arabinoxylans in grist containing wheat malt using response surface methodology. J Am Soc Brew Chem 63:171–176
Li Y, Liu Z, Cui F, Xu Y, Zhao H (2007a) Application of statistical experimental design to optimize culture requirements of a novel Aspergillus sp. ZH-26 producing endoxylanase from agricultural waste material and evaluation of its performance on the degradation of arabinoxylans in mashing. J Food Sci 72:320–329. doi:10.1111/j.1750-3841.2007.00389.x
Li Y, Liu Z, Zhao H, Xu YY, Cui FJ (2007b) Statistical optimization of xylanase production from new isolated Penicillium oxalicum ZH-30 in submerged fermentation. Biochem Eng J 34:82–86. doi:10.1016/j.bej.2006.11.011
Liu ZQ, Li Y, Ping LF, Xu YY, Cui FG, Zheng YG (2007) Isolation and identification of a novel Rhodococcus sp. ML-0004 producing epoxide hydrolase and optimization of enzyme production. Process Biochem 42:889–894. doi:10.1016/j.procbio.2007.01.009
Liu ZQ, Hu ZC, Zheng YG, Shen YC (2008a) Optimization of cultivation conditions for the production of 1, 3-dihydroxyacetone by Pichia membranifaciens using response surface methodology. Biochem Eng J 38:285–291. doi:10.1016/j.bej.2007.07.015
Liu ZQ, Zhang JF, Zheng YG, Shen YC (2008b) Production of astaxanthin from a newly isolated phaffia rhodozyma mutant by low-energy ions beam implantation. J Appl Microbiol 108:861–872. doi:10.1111/j.1365-2672.2007.03603.x
Lu J, Li Y, Gu GX, Mao ZG (2005) Effects of molecular weight and concentration of arabinoxylans on the membrane plugging. J Agric Food Chem 53:4996–5002. doi:10.1021/jf0480694
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem 31:426–428. doi:10.1021/ac60147a030
Silva CJSM, Roberto IC (2001) Optimization of xylitol production by Candida guilliermondi FTI 20037 using response surface methodology. Process Biochem 36:1119–1124. doi:10.1016/S0032-9592(01)00153-4
Acknowledgments
The authors wish to thank the Technological Research and Development Program of Zhenjiang City, Jiangsu Education Department Natural Sciences Foundation of China (07KJD350034), Natural Science Foundation of Zhejiang Province (No. Y506136) and Research Foundation for Advanced Talents of Jiangsu University (07JDG020) for their financial supports.
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Cui, F., Li, Y., Liu, Z. et al. Optimization of fermentation conditions for production of xylanase by a newly isolated strain, Penicillium thiersii ZH-19. World J Microbiol Biotechnol 25, 721–725 (2009). https://doi.org/10.1007/s11274-008-9942-9
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DOI: https://doi.org/10.1007/s11274-008-9942-9