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Quantification of indole-3-acetic acid from plant associated Bacillus spp. and their phytostimulatory effect on Vigna radiata (L.)

Abstract

Sixteen Bacillus strains isolated from rhizosphere, histoplane and phyllosphere of different plant species were identified by 16S rDNA gene sequencing and evaluated for in vitro auxin production as well as growth stimulation of Vigna radiata (L.) Wilczek. Auxin production by Bacillus spp. in L-broth medium supplemented with 1,000 μg ml−1 L-tryptophan ranges from 0.60 to 3.0 μg IAA ml−1 as revealed by gas chromatography and mass spectrometric (GC–MS) analysis. Rhizospheric isolates exhibit relatively more IAA synthesis than histoplane and phyllosphere isolates. Plant microbe interaction experiments conducted under gnotobiotic conditions recorded 55.55, 46.46 and 46.20% increase in shoot length with Bacillus megaterium MiR-4, B. pumilus NpR-1 and B. subtilis TpP-1, respectively, over control. Bacillus inoculations also increased shoot fresh weight with B. megaterium MiR-4 (60.94%) and B. pumilus NpR-1 (37.76%). Highly significant positive correlation between auxin production analyzed by GC–MS and shoot length (r = 0.687**, P = 0.01) and shoot fresh weight (r = 0.703**, P = 0.01) was noted under gnotobiotic conditions. Similarly, significant correlation was also found between auxin production by Bacillus spp. (GC–MS analysis) and different growth parameters such as shoot length (r = 0.495*, P = 0.05), number of pods (r = 0.498*, P = 0.05) and grain weight (r = 0.537*, P = 0.05) at full maturity under natural wire house conditions. Results showed that auxin production potential of plant associated Bacillus spp. can be effectively exploited to enhance the growth and yield of V. radiata.

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Acknowledgments

Higher Education Commission of Pakistan is acknowledged for providing funding to Basharat Ali under international research support initiative program (No.1–8/HEC/HRD/2007/471-VI) to visit Umeå Plant Science Centre, Umeå, Sweden to do GC–MS quantification of bacterial indole-3-acetic acid. Roger Granbom is acknowledged for help with GC–MS analysis.

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Correspondence to Shahida Hasnain.

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Ali, B., Sabri, A.N., Ljung, K. et al. Quantification of indole-3-acetic acid from plant associated Bacillus spp. and their phytostimulatory effect on Vigna radiata (L.). World J Microbiol Biotechnol 25, 519 (2009). https://doi.org/10.1007/s11274-008-9918-9

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  • DOI: https://doi.org/10.1007/s11274-008-9918-9

Keywords

  • Bacillus
  • In vitro
  • L-tryptophan
  • Auxin
  • GC–MS analysis
  • Gnotobiotic conditions