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The purification and characterization of deoxycytidine kinase from calf thymus

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Abstract

A facile and fast approach for the purification of deoxycytidine kinase (dCK) from calf thymus was developed using a fast performance liquid chromatography system. A 73-fold enrichment of the enzyme was noted compared to unfractionated dCK. Characterization studies demonstrated that dCK had a molecular mass of 31 kDa using SDS–PAGE, an optimum pH of 7.0 and the enzyme maintained stability between 30 and 40°C. The rapid preparation of dCK demonstrated here will be valuable in the synthesis of nucleotide analogs.

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Acknowledgments

This work was supported by program for Changjiang Scholars and Innovative Research Team in Donghua University (No. IRT0526). UK-CHINA Joint Laboratory for Therapeutic Textiles based in Donghua University, in part by National Science Foundation Committee of China (50773009) and Biomedical Textile Materials “111 Project”, Ministry of Education of P. R. China.

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Authors and Affiliations

  1. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 201620, Shanghai, Peoples’ Republic of China

    Jing Quan, Yan-Qian Chai & Li-Min Zhu

  2. Institute for Health Research and Policy, London Metropolitan University, 166-220, Holloway Road, London, N78PB, UK

    Christopher J. Branford-White

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  1. Jing Quan
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  2. Yan-Qian Chai
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  3. Christopher J. Branford-White
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  4. Li-Min Zhu
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Correspondence to Li-Min Zhu.

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Quan, J., Chai, YQ., Branford-White, C.J. et al. The purification and characterization of deoxycytidine kinase from calf thymus. World J Microbiol Biotechnol 25, 475–480 (2009). https://doi.org/10.1007/s11274-008-9912-2

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  • DOI: https://doi.org/10.1007/s11274-008-9912-2

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