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Statistical analysis and optimization of nitrogen, phosphorus, and inoculum concentrations for the biodegradation of petroleum hydrocarbons by response surface methodology

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Abstract

In order to optimize and evaluate the influence of nitrogen, phosphorus, and inoculum concentrations on the biodegradation of hydrocarbon contaminated effluents, experiments based on central composite design (CCD) method were carried out for 3 days, employing C1 mixed culture and intermittent aeration. The independent variables were nitrogen concentration (X 1), phosphorus concentration (X 2), and inoculum concentration (X 3) and the removal of total petroleum hydrocarbons (TPH) was the dependent variable. The optimized nutrients ratio (C:N:P = 100:20:2.7) and inoculum concentration (1.32 g/l) provided TPH removal of 71.8% after processing for three days. Analysis using gas chromatography identified five hydrocarbons classes: paraffins, isoparaffins, olefins, naphthenics, and aromatics. The naphthenic compounds did not degrade as readily as the other hydrocarbons that were identified. The following degradation percentages were obtained: 87.1% for the paraffins, 77.7% for the isoparaffins, 78.6% for the olefins, 38.4% for the naphthenics, and 71.7% for the aromatics.

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References

  • ABNT/NBR 9898 (1987) Preservação e técnicas de amostragem de efluentes líquidos e corpos receptores, Rio de Janeiro-RJ, 22p

  • Alexander M (1999) Biodegradation and bioremediation. Academic Press, San Diego, USA, p 453

    Google Scholar 

  • Antonio GC, Kurozawa LE, Murr FEX, Park KJ (2006) Optimization of the osmotic dehydration of sweet potato (Ipomoea batatas) using response surface methodology. Braz J Food Technol 9:135–141

    CAS  Google Scholar 

  • Atlas RM (1995) Bioremediation of petroleum pollutants. Int Biodeterior Biodegrad 35:317–327

    Article  CAS  Google Scholar 

  • Boopathy R (2004) Anaerobic biodegradation of no, 2 diesel fuel in soil: a soil column study. Bioresour Technol 94:143–151. doi:10.1016/j.biortech.2003.12.006

    Article  CAS  Google Scholar 

  • Byunghoon K, Myunghee H, Sungyong C, Sungjin A, Sung-Paal L, Sunkyun Y (2003) Statistical optimization for biodegradation of 2, 4-dichlorophenoxyacetic acid by soil isolated bacterium. Korean Soc Microbiol Biotechnol 31:83–89

    Google Scholar 

  • Chapelle FH (1999) Bioremediation of petroleum hydrocarbon-contaminated ground water: the perspectives of history and hydrology. Ground Water 37:122–132. doi:10.1111/j.1745-6584.1999.tb00965.x

    Article  CAS  Google Scholar 

  • Cliff N, Krus DJ (1976) Interpretation of canonical variate analysis: rotated vs unrotated solutions. Psychometrika 41:35–42. doi:10.1007/BF02291696

    Article  Google Scholar 

  • Coulon F, Pelletier E, Gourbant L, Delille D (2005) Effects of nutrient and temperature on degradation of petroleum hydrocarbons in contaminated sub Antartic soil. Chemosphere 58:1439–1448. doi:10.1016/j.chemosphere.2004.10.007

    Article  CAS  Google Scholar 

  • Cunha CD, Leite SGF (2000) Gasoline biodegradation in different soil microcosms. Braz J Microbiol 31:45–49. doi:10.1590/S1517-83822000000100011

    Article  Google Scholar 

  • Debbrecht FJ (1985) Qualitative and quantitative analysis by gas chromatography. In: Grob RL (ed) Modern practice of gas chromatography. Wiley, New York, pp 359–421

    Google Scholar 

  • Deuel LE, Holliday GH (1997) Soil remediation for the petroleum extraction industry. Penn Well, Tulsa, USA, p 242

    Google Scholar 

  • Ferguson SH, Franzmann PD, Revill AT, Spane I, Rayner JL (2003) The effects of nitrogen and water on mineralisation of hydrocarbons in diesel-contaminated terrestrial antartics soils. Cold Reg Sci Technol 37:197–212. doi:10.1016/S0165-232X(03)00041-7

    Article  Google Scholar 

  • Ghazali FM, Abdul Rahman RNZ, Salleh AB, Basri M (2004) Biodegradation of hydrocarbons in soil by microbial consortiu. Int Biodeterior Biodegrad 54:61–67. doi:10.1016/j.ibiod.2004.02.002

    Article  CAS  Google Scholar 

  • Kornmann H, Valentinotti S, Duboc P, Marison I, Stockar UV (2004) Monitoring and control of gluconacetobacter xylinus fed-batch cultures using in situ mid-IR spectroscopy. J Biotechnol 113:231–245. doi:10.1016/j.jbiotec.2004.03.029

    Article  CAS  Google Scholar 

  • Leahy JG, Colwell RR (1990) Microbial degradation of hydrocarbons in the environment. Microbiol Rev 54:305–315

    CAS  Google Scholar 

  • Lee SH, Lee S, Kim DY, Kim JG (2007) Degradation characteristics of waste lubricants under different nutrient conditions. J Hazard Mater 143:65–72. doi:10.1016/j.jhazmat.2006.08.059

    Article  CAS  Google Scholar 

  • Liebeg EW, Cutright TJ (1999) The investigation of bioremediation through the addition of macro and micro nutrients in PAH contaminates soil. Int Biodeterior Biodegrad 44:55–64. doi:10.1016/S0964-8305(99)00060-8

    Article  CAS  Google Scholar 

  • Mariano AP, Kataoka APAG, Angelis DF, Bonotto DM (2007) Laboratory study on the bioremediation of diesel oil contaminated soil from a petrol station. Braz J Microbiol 38:346–353. doi:10.1590/S1517-83822007000200030

    Article  Google Scholar 

  • Márquez-Rocha FJ, Olmos-Solto J, Rosano-Hernández Ma C, Muriel-García M (2005) Determination of the hydrocarbon-degrading metabolic capabilities of tropical bacterial isolates. Int Biodeterior Biodegrad 55:17–23. doi:10.1016/j.ibiod.2004.05.007

    Article  Google Scholar 

  • Mrayyan B, Battikhi MN (2005) Biodegradation of total organic carbons (TOC) in Jordain petroleum sludge. J Hazard Mater 120:127–134. doi:10.1016/j.jhazmat.2004.12.033

    Article  CAS  Google Scholar 

  • Margesin R, Schinner F (2001) Bioremediation (natural attenuation and biostimulation) of diesel-oil contaminated soil in an alpine glacier skiing area. Appl Environ Microbiol 67:3127–3133. doi:10.1128/AEM.67.7.3127-3133.2001

    Article  CAS  Google Scholar 

  • Moeri E, Coelho R, Markei A (2004) Remediação e Revitalização de Áreas Contaminadas. Aspectos Técnicos Legais e Financeiros. Editora Signus, 219p

  • Nanda V, Bera MB, Bakhshi AK (2006) Optimization of the process parameters to establish the quality attributes of hydroxymethylfurfural content and diastatic activity of sunflower (Helianthus annus) honey using response surface methodology. Eur Food Res Technol 222:64–70. doi:10.1007/s00217-005-0133-8

    Article  CAS  Google Scholar 

  • Prince RC, Parkerton TF, Lee C (2007) The primary aerobic biodegradation of gasoline hydrocarbons. Environ Sci Technol 41:3316–3321. doi:10.1021/es062884d

    Article  CAS  Google Scholar 

  • Rahman KSM, Banat IM, Thahira J, Thayumanavan TF, Lakshmanaperumalsamy P (2002) Bioremediation of gasoline contaminated soil by a bacterial consortium amend with poultry litter, coir pith and rhaminolipid biosurfactant. Bioresour Technol 81:25–32. doi:10.1016/S0960-8524(01)00105-5

    Article  CAS  Google Scholar 

  • Rodrigues MI, Iemma AF (2005) Planejamento de experimentos e otimização de processos. In: Casa do Pão (ed) Uma estratégia seqüencial de planejamento. Campinas - São Paulo, Brasil, pp 135–215

  • Sohrabi M, Mogharei A (1999) Some aspects of bioremediation of soil contaminad with petroleum hydrocarbons. Afinidad LVI, 483p

  • Solano-Serena F, Marchal R, Ropars M, Lebecurt JM, Vandecostelle JP (1999) Biodegradation of gasoline: kinetics, mass balance and fate of individuall hydrocarbons. J Appl Microbiol 86:1008–1016. doi:10.1046/j.1365-2672.1999.00782.x

    Article  CAS  Google Scholar 

  • Spain JC, Van Veld PA (1983) Adaptation of natural microbial communities to degradation of xenobiotic compounds: effects of concentration, exposure time, inoculum, and chemical structure. Appl Environ Microbiol 45:428–435

    CAS  Google Scholar 

  • Strynar M, Sen D, Weaver Rw (1999) Nitrogen and phosphorus for growth of oil-degrading microorganisms in seawater. Departament of Soil and Crop Sciences, Texas A & M University, College Station, TX77843-2474

  • Townsend GT, Prince RC, Suflita JM (2004) Anaerobic biodegradation of alicyclic constituents of gasoline and natural gas condensate by bacteria from an anoxic aquifer. FEMS Microbiol Ecol 49:129–135. doi:10.1016/j.femsec.2003.08.015

    Article  CAS  Google Scholar 

  • Trindad PVO, Sobral LG, Rizzo ACL, Leite SGF, Lemos JLS, Milloili VS, Soriano AU (2002) Evaluation of the biostimulation and bioaugmentation techniques in the bioremediation process of petroleum hydrocarbon contaminated soils. 9th annual international petroleum environmental conference. Novo México. http://ipec.utulsa.edu/Conf2002/trindade_soriano_21.pdf

  • Verma P, Agrawal US, Sharma AK, Sarkar BC, Sharma HK (2005) Optimization of process parameters for the development of a cheese analogue from pigeon pea (Cajanus cajan) and soy milk using response surface methodology. Int J Dairy Technol 58:51–58. doi:10.1111/j.1471-0307.2005.00182.x

    Article  Google Scholar 

  • Vieira PA, Vieira RB, De França FP, Cardoso VL (2007) Biodegradation of effluent contaminated with diesel fuel and gasoline. J Hazard Mater 140:52–59. doi:10.1016/j.jhazmat.2006.06.048

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais).

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Authors and Affiliations

  1. Universidade Federal de Uberlândia, Faculdade de Engenharia Química, Campus Santa Mônica, Bloco 1 K, Caixa Postal 593, CEP 38400-902, Uberlândia, MG, Brasil

    P. A. Vieira, S. Faria, R. B. Vieira & V. L. Cardoso

  2. Universidade Federal do Rio de Janeiro, Escola de Química, Caixa Postal 68502, CEP 21945-970, Rio de Janeiro, RJ, Brasil

    F. P. De França

Authors
  1. P. A. Vieira
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  2. S. Faria
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  3. R. B. Vieira
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  4. F. P. De França
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  5. V. L. Cardoso
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Correspondence to P. A. Vieira.

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Vieira, P.A., Faria, S., Vieira, R.B. et al. Statistical analysis and optimization of nitrogen, phosphorus, and inoculum concentrations for the biodegradation of petroleum hydrocarbons by response surface methodology. World J Microbiol Biotechnol 25, 427–438 (2009). https://doi.org/10.1007/s11274-008-9907-z

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  • DOI: https://doi.org/10.1007/s11274-008-9907-z

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