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Extracellular hydrolytic enzyme screening of culturable heterotrophic bacteria from deep-sea sediments of the Southern Okinawa Trough

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Abstract

The Southern Okinawa Trough is an area of focused sedimentation due to particulate matter export from the shelf of the East China Sea and the island of Taiwan. In order to understand the geomicrobiological characteristics of this unique sedimentary environment, bacterial cultivations were carried out for an 8.61 m CASQ core sediment sample. A total of 98 heterotrophic bacterial isolates were characterized based on 16S rRNA gene phylogenetic analysis. These isolates can be grouped into four bacterial divisions, including 13 genera and more than 20 species. Bacteria of the γ-Proteobacteria lineage, especially those from the Halomonas (27 isolates) and Psychrobacter (20 isolates) groups, dominate in the culturable bacteria assemblage. They also have the broadest distribution along the depth of the sediment. More than 72.4% of the isolates showed extracellular hydrolytic enzyme activities, such as amylases, proteases, lipases and Dnases, and nearly 59.2% were cold-adapted exoenzyme-producers. Several Halomonas strains show almost all the tested hydrolases activities. The wide distribution of exoenzyme activities in the isolates may indicate their important ecological role of element biogeochemical cycling in the studied deep-sea sedimentary environment.

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Acknowledgments

The sediment samples used in this study were collected during the MD147/MARCO POLO 1/IMAGES XII cruise of the R/V Marion Dufresne of the French Polar Institute (IPEV). This work was financially supported by the Pilot Projects of Knowledge Innovation Project of Chinese Academy of Sciences grants KZCX2-YW-211-03, KZCX3-SW-233 and KZCX3-SW-223, the National Natural Science Foundation of China grant 40576069, the Hi-Tech Research and Development Program of China grant 2007AA091903, and the China Ocean Mineral Resources R and D Association grants DYXM-115-02-2-6 and DYXM-115-02-2-20.

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Authors and Affiliations

  1. Key Laboratory of Marine Geology and Environment, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Hongyue Dang & Tiegang Li

  2. Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, 266555, China

    Hongyue Dang, Hu Zhu & Jing Wang

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  1. Hongyue Dang
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Correspondence to Hongyue Dang or Tiegang Li.

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Dang, H., Zhu, H., Wang, J. et al. Extracellular hydrolytic enzyme screening of culturable heterotrophic bacteria from deep-sea sediments of the Southern Okinawa Trough. World J Microbiol Biotechnol 25, 71–79 (2009). https://doi.org/10.1007/s11274-008-9865-5

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