Abstract
Selected starter cultures of Oenococcus oeni are widely used to initiate malolactic fermentation (MLF) in wine. Nevertheless, the inoculated culture does not always develop as expected and undesired strains can grow causing wine spoilage. Therefore, methods that can reliably differentiate O. oeni strains are essential to monitor the population dynamics of MLF. This work presents a new multiplex PCR method that allows the simultaneous species identification and strain typification of O. oeni, based on the combined use of species-specific PCR primers and a Random Polymorphic DNA (RAPD)-PCR primer. This method represents an useful tool for the control of wine MLF.
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References
Cocconcelli PS, Porro D, Galandini S, Senini L (1995) Development of RAPD protocol for typing of strains of lactic acid bacteria and enterococci. Lett Appl Microbiol 21:376–379. doi:10.1111/j.1472-765X.1995.tb01085.x
De Man JC, Rogosa M, Sharpe ME (1960) A medium for the cultivation of lactobacilli. J Appl Bacteriol 23:130–135
Henick-Kling T (1993) Malolactic fermentation. In: Fleet GH (ed) Wine microbiology and biotechnology. Harwood Academic Publishers, Chur, Switzerland, pp 289–326
Guerrini S, Bastianini A, Blaiotta G, Granchi L, Moschetti G, Coppolla S et al (2003) Phenotypic and genotypic characterization of Oenococcus oeni strains isolated from Italian wines. Int J Food Microbiol 83:1–14. doi:10.1016/S0168-1605(02)00323-9
Lechiancole T, Blaiotta G, Messina D, Fusco V, Villani F, Salzano G (2006) Evaluation of intra-specific diversities in Oenococcus oeni through analysis of genomic and expressed DNA. Syst Appl Microbiol 29:375–381. doi:10.1016/j.syapm.2005.10.001
Lonvaud-Funel A (1999) Lactic acid bacteria in the quality improvement and depreciation of wine. Antonie Van Leeuwenhoek 76:317–331. doi:10.1023/A:1002088931106
Maicas S, Pardo I, Ferrer S (2000) The effects of freezing and freeze-drying of Oenococcus oeni upon induction of malolactic fermentation in red wine. Int J Food Microbiol 35:75–79
Reguant C, Bordons A (2003) Typification of Oenococcus oeni strains by multiplex RAPD-PCR and study of population dynamics during malolactic fermentation. J Appl Microbiol 95:344–353. doi:10.1046/j.1365-2672.2003.01985.x
Sakar G, Kapelner S, Sommer SS (1990) Formamide can dramatically improve the specificity of PCR. Nucleic Acids Res 18:7465. doi:10.1093/nar/18.24.7465
Spano G, Lonvaud-Funel A, Claisse O, Massa S (2007) In vivo PCR-DGGE analysis of Lactobacillus plantarum and Oenococcus oeni populations in red wine. Curr Microbiol 54:9–13. doi:10.1007/s00284-006-0136-0
Van Vuuren HJJ, Dicks LMT (1993) Leuconostoc oenos: a review. Am J Enol Vitic 44:99–112
Zapparoli G, Torriani S, Pesente P, Dellaglio F (1998) Design and evaluation of malolactic enzyme gene targeted primers for rapid identification and detection of Oenococcus oeni in wine. Lett Appl Microbiol 27:243–246. doi:10.1046/j.1472-765X.1998.00448.x
Zapparoli G, Reguant C, Bordons A, Torriani S, Dellaglio F (2000) Genomic DNA fingerprinting of Oenococcus oeni strains by pulsed-field electrophoresis and randomly amplified polymorphic DNA-PCR. Curr Microbiol 40:351–355. doi:10.1007/s002840010069
Zavaleta AI, Martínez-Murcia AJ, Rodríguez-Valera F (1996) 16S–23S DNA intergenic sequences indicate that Leuconostoc oenos is phylogenetically homogeneous. Microbiology 142:2105–2114
Acknowledgements
This work was supported by grants AGL2000-0827-C02-02 and AGL2006-3700 from the Spanish Ministerio de Ciencia y Tecnología.
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Araque, M.I., Bordons, A. & Reguant, C. A multiplex PCR method for simultaneous species identification and strain typification of Oenococcus oeni . World J Microbiol Biotechnol 25, 15–18 (2009). https://doi.org/10.1007/s11274-008-9854-8
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DOI: https://doi.org/10.1007/s11274-008-9854-8