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Incidence of diverse integrons and β-lactamase genes in environmental Enterobacteriaceae isolates from Jiaozhou Bay, China

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Abstract

Environmental microbiology investigation was performed to determine the molecular diversity of β-lactamase genes among ampicillin-resistant bacteria from Jiaozhou Bay. β-lactamase genes were detected in 93.8% of the bacterial isolates identified as Enterobacteriaceae. The most frequently detected gene was bla TEM, followed by bla SHV, bla OAX-1, bla MOX and bla CMY. Most of the isolates (68.8%) were positive for the intI1 integrase gene, and two isolates were also found for the intI2 gene. The dfr and aadA gene cassettes were predominant. Anthropogenic contamination from onshore sewage processing plants might contribute predominantly to the β-lactamase gene reservoir in the studied coastal waters. Environmental antibiotic-resistant bacteria and resistance genes may serve as bioindicators of coastal environmental quality or biotracers of the potential contamination sources. This is the first report of the prevalence and characterization of β-lactamase genes and integrons in coastal Enterobacteriaceae from China.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China grants (NSFC Grant Nos. 40576069 and 40476058), the Hi-Tech Research and Development Program of China grant 2007AA091903, the China Ocean Mineral Resources R&D Association grants DYXM-115-02-2-6 and DYXM-115-02-2-20.

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Authors and Affiliations

  1. College of Environmental Science and Engineering, Dalian Maritime University, Dalian, 116026, China

    Chunyan Wang

  2. Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, 266555, China

    Chunyan Wang & Hongyue Dang

  3. Key Laboratory of Marine Geology and Environment, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Chunyan Wang & Hongyue Dang

  4. College of Marine Environment and Safety Engineering, Shanghai Maritime University, Shanghai, 200135, China

    Yongsheng Ding

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  1. Chunyan Wang
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  2. Hongyue Dang
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Correspondence to Hongyue Dang.

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Wang, C., Dang, H. & Ding, Y. Incidence of diverse integrons and β-lactamase genes in environmental Enterobacteriaceae isolates from Jiaozhou Bay, China. World J Microbiol Biotechnol 24, 2889–2896 (2008). https://doi.org/10.1007/s11274-008-9827-y

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