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Influence of Zn2+, Co2+ and dimethylbenzimidazole on vitamin B12 biosynthesis by Pseudomonas denitrificans

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Previous research has confirmed that cobalt ion and dimethylbenzimidazole (DMBI) are the precursors of vitamin B12 biosynthesis, and porphobilinogen synthase (PBG synthase) is a zinc-requiring enzyme. In this paper, the effects of Zn2+, Co2+ and DMBI on vitamin B12 production by Pseudomonas denitrificans in shake flasks were studied. Present experimental results demonstrated that the addition of the above mentioned three components to the fermentation medium could significantly stimulate the biosynthesis of vitamin B12. The concentrations of zinc sulphate, cobaltous chloride and DMBI in the fermentation medium were further optimized with rotatable orthogonal central composite design and statistical analysis by Data Processing System (DPS) software. As a result, vitamin B12 production was increased from 69.36 ± 0.66 to 78.23 ± 0.92 μg/ml.

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δ-Aminolevulinic acid




Data Processing System


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This work was supported by National Basic Research Program (973 Program, No. 2007CB714303) and Shanghai Leading Academic Discipline Project (B505). We are grateful to Shijiazhuang Pharma.Group Hua Rong Pharmaceutical Co. Ltd for supporting this work.

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Correspondence to Si-Liang Zhang.

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Li, KT., Liu, DH., Zhuang, YP. et al. Influence of Zn2+, Co2+ and dimethylbenzimidazole on vitamin B12 biosynthesis by Pseudomonas denitrificans . World J Microbiol Biotechnol 24, 2525–2530 (2008).

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