Skip to main content
SpringerLink
Log in

Biological denitrification by Pseudomonas stutzeri immobilized on microbial cellulose

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

This study demonstrated the feasibility of a biological denitrification process using immobilized Pseudomonas stutzeri. The microbial cellulose (MC) from Acetobacter xylinum was used as the support material for immobilization of the bacterium. Nitrate removal took place mainly in the anoxic system. The effects of various operating conditions such as the initial nitrate concentration, pH, and carbon source on biological denitrification were demonstrated experimentally. The system demonstrated a high capacity for reducing nitrate concentrations under optimum conditions. The denitrification rate increased up to a maximal value of 1.6 kg NO3-N m−3 day−1 with increasing nitrate loading rate. Because of its porosity and purity, MC may be considered as appropriate supports for adsorbed immobilized cells. The simplicity of immobilization and high efficiency in operation are the main advantages of such systems. To date, the immobilization of microorganisms onto MC has not been carried out. The results of this research shows that a pilot bioreactor containing P. stutzeri immobilized on MC exhibited efficient denitrification with a relatively low retention time.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • APHA, AWW, WPCF (2005) Standard methods for the examination of water and wastewater, 21th edn. American Public Health Association, Washington

    Google Scholar 

  • Aslan S, Cakici H (2007) Biological denitrification of drinking water in a slow sand filter. J Hazard Mater 148:253–258. doi:10.1016/j.jhazmat.2007.02.012

    Article  CAS  Google Scholar 

  • Astley OM, Chanliaud E, Donald AM, Gidley MJ (2001) Structure of Acetobacter cellulose composites in the Hydrated State. Int J Biol Macromol 29:193–202. doi:10.1016/S0141-8130(01)00167-2

    Article  CAS  Google Scholar 

  • Bäckdahl H, Helenius G, Bodin A, Nannmark U (2006) Mechanical properties of bacterial cellulose and interactions with smooth muscle cells. Biomaterials 27:2141–2149. doi:10.1016/j.biomaterials.2005.10.026

    Article  Google Scholar 

  • Bernat K, Wojnowska-Baryla I (2007) Carbon source in aerobic denitrification. Biochem Eng J 36:116–122. doi:10.1016/j.bej.2007.02.007

    Article  CAS  Google Scholar 

  • Chen KC, Lee SC, Chin SC, Houng JY (1998) Simultaneous carbon-nitrogen removal in wastewater using phosphorylated PVA-immobilized microorganisms. Enzyme Microb Technol 23:311–320. doi:10.1016/S0141-0229(98)00054-4

    Article  CAS  Google Scholar 

  • Chibata I, Wingard LB (1983) Immobilized microbial cells. In: Wingard LB et al (eds) Applied biochemistry and bioengineering, vol 4. Academic Press, New York

    Google Scholar 

  • Dawson RN, Murphy KL (1972). The temperature dependency of biological denitrification. Water Res 6:71–83. doi:10.1016/0043-1354(72)90174-1

    Article  CAS  Google Scholar 

  • Fan AM, Steinberg VE (1996) Health implications on nitrate and nitrite in drinking water: an update on methaemoglobinemia occurrence and reproductive and development toxicity. Regul Toxicol Pharmacol 23:35–43. doi:10.1006/rtph.1996.0006

    Article  CAS  Google Scholar 

  • George, j, Ramana KV, Sabapathy SN, Bawa AS (2005) Physico-mechanical properties of chemically treated bacterial (Acetobacter xylinum) cellulose membrane. World J Microb Biot 21:1323–1327. doi:10.1007/s11274-005-3574-0

    Article  CAS  Google Scholar 

  • Kariminiaae-Hamedaani HR, Kanda K, Kato F (2004) Denitrification activity of the bacterium Pseudomonas sp ASM-2-3 isolated from the Ariake Sea tideland. J Biosci Bioeng 97:1–88

    Google Scholar 

  • Kesseri P, Kiss I, Bihari Z, Polyak B (2002) Investigation of the denitrification activity of immobilized Pseudomonas butanovora cells in the presence of different organic substrates. Water Res 36:1565–1571. doi:10.1016/S0043-1354(01)00364-5

    Article  Google Scholar 

  • Klemm D, Schumann D, Udhardt U, Marsch S (2001) Bacterial synthesized cellulose—artificial blood vessels for microsurgery. Prog Polym Sci 26:1561–1603. doi:10.1016/S0079-6700(01)00021-1

    Article  CAS  Google Scholar 

  • Krieg NR, Holt JG (1984) Bergey’s manual of systematic bacteriology, vol 1. Williams and Wilkins, Baltimore

    Google Scholar 

  • Lazarova V, Manem J (1995). Biofilm characterization and activity analysis in water and wastewater treatment. Water Res 29:2227–2245. doi:10.1016/0043-1354(95)00054-O

    Article  CAS  Google Scholar 

  • Manohar S, Karegoudar TB (1998) Degradation of naphthalene by cells of Pseudomonas sp. strain NGK 1 immobilized in alginate, agar and polyacrylamide. Appl Microbiol Biotechnol 49:785–792

    Article  CAS  Google Scholar 

  • Mohseni-Bandpi A, Elliott DJ, Momeny-Mazdeh A (1999) Denitrification of groundwater using acetic acid as a carbon source. Water Sci Technol 40:53–59. doi:10.1016/S0273-1223(99)00430-8

    Article  CAS  Google Scholar 

  • Oh SE, Kim KS, Choi HC (2000) Kinetics and physiological characteristics of autotrophic denitrification by denitrification sulphur bacteria. Water Sci Technol 42:59–63

    CAS  Google Scholar 

  • Peng YZ, Yong MA, Wang SY (2007) DenitrificationTT potential enhancement by addition of external carbon sources in a pre-TTdenitrificationTT process. J Environ Sci 19:284–289. doi:10.1016/S1001-0742(07)60046-1

    Article  CAS  Google Scholar 

  • Saliling WJB, Westerman PW, Losordo TM (2007) Wood chips and wheat straw as alternative biofilter media for denitrification reactors treating aquaculture and other wastewaters with high nitrate concentrations. Aquac Eng 37:222–233. doi:10.1016/j.aquaeng.2007.06.003

    Article  Google Scholar 

  • Sawant SS, Prabhudessai L, Venkat K (2007) Eutrophication status of marine environment of Mumbai and Jawaharlal Nehru ports. Environ Monit Assess 127:283–291

    Article  CAS  Google Scholar 

  • Son HJ, Kim HG, Kim KK (2003). Increased production of bacterial cellulose by Acetobacter sp. V6 in synthetic media under shaking culture conditions. Bioresour Technol 86:215–219. doi:10.1016/S0960-8524(02)00176-1

    Article  Google Scholar 

  • Svensson A, Nicklasson E, Harrah T, Panilaitis B, Kaplan DL (2005) Bacterial cellulose as a potential scaffold for tissue engineering of cartilage. Biomaterials 26:419–431. doi:10.1016/j.biomaterials.2004.02.049

    Article  CAS  Google Scholar 

  • Vrtovsek J, Ros M, Milenko R (2006) Denitrification of ground water in the biofilm reactor with a specific biomass support material. Acta Chim Slov 53:396–400

    CAS  Google Scholar 

  • Wang J, Hou W, Qian Y (1995) Immobilization of microbial cells using polyvinyl alcohol (PVA)—polyacrylamide gels. Biotechnol Tech 9:203–208

    Article  Google Scholar 

  • Yamagiwa K, Kozawa T, Nakamura A, Ohkawa A (1997) Immobilization of denitrifier within a matrix with low oxygen permeability. Biotechnol Tech 11:95–98. doi:10.1023/A:1018468506352

    Article  CAS  Google Scholar 

  • Yang CY, Wu DC, Chang CC (2007). Nitrate in drinking water and risk of death from colon cancer in Taiwan. Environ Int 33:649–653. doi:10.1016/j.envint.2007.01.009

    Article  Google Scholar 

Download references

Acknowledgment

The authors would like to thank Eng. Rezaee for his kind assistance with the SEM.

Author information

Authors and Affiliations

  1. Department of Environmental Health, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Abbas Rezaee, Hatam Godini, Said Dehestani, Ahmad Reza Yazdanbakhsh, Gholamreza Mosavi & Anoshiravan Kazemnejad

Authors
  1. Abbas Rezaee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hatam Godini
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Said Dehestani
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ahmad Reza Yazdanbakhsh
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Gholamreza Mosavi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Anoshiravan Kazemnejad
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Abbas Rezaee.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rezaee, A., Godini, H., Dehestani, S. et al. Biological denitrification by Pseudomonas stutzeri immobilized on microbial cellulose. World J Microbiol Biotechnol 24, 2397–2402 (2008). https://doi.org/10.1007/s11274-008-9753-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-008-9753-z

Keywords

Search

Navigation