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Leakage of recombinant penicillin G acylase from Escherichia coli by adding chloroform under fermentation conditions

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Abstract

A simple method was developed to release periplasmic penicillin G acylase from Escherichia coli BL21(DE3) during the fermentation process. More than 80% of the total penicillin G acylase was released into the broth when 3% (v/v) chloroform was added at 3 h after induction. The activity of extracellular penicillin G acylase reached 20699 U/l. This method was efficient and would facilitate further investigation of penicillin G acylase for industrial applications.

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Acknowledgments

This work was supported by grant no. 2004AA2Z3801 from High Tech Research and Development (863) Program of the Ministry of Science and Technology of China and the Program for New Century Excellent Talents in University (project No. NCET-04-0411).

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, Institute of Newworld Biotechnology, East China University of Science and Technology, P.O.Box 311, Shanghai, 200237, China

    Yongmei Jiang & Dongzhi Wei

  2. College of Life Science, Fujian Nomal University, Fuzhou, 350007, China

    Yongmei Jiang

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  1. Yongmei Jiang
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  2. Dongzhi Wei
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Correspondence to Dongzhi Wei.

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Jiang, Y., Wei, D. Leakage of recombinant penicillin G acylase from Escherichia coli by adding chloroform under fermentation conditions. World J Microbiol Biotechnol 24, 2365–2367 (2008). https://doi.org/10.1007/s11274-008-9738-y

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  • DOI: https://doi.org/10.1007/s11274-008-9738-y

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