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Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor

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Abstract

The study was done to improve the viability of the RC1 hybridoma cell in order to produce more amount of monoclonal antibody (mAb). By using the optimized media, the cell had been cultured in two bioreactor systems which were the MiniPerm and Stirred Tank bioreactor (ST bioreactor), and the results were compared to the one obtained by using the T-Flask bioreactor which was used as a standard. The results showed that the ST bioreactor was able to improve the viability of the cell to the value of 91.8% which was a little bit better than the one obtained by the MiniPerm bioreactor (88.6%) and far better than that of achieved by the T-Flask bioreactor (76.4%). This was well correlated with the good growth performance of the cell in the ST bioreactor with the specific growth rate (μ) value of 0.0289 h−1 followed by MiniPerm bioreactor with the value of 0.0243 h−1 and then the T-Flask with the value of 0.0151 h−1. The low value of doubling time (t d ) obtained in the ST bioreactor (24 h) compared to the one obtained in the MiniPerm (29 h) and T-Flask bioreactor (46 h) had also contributed to the higher value of cell viability. As a result a higher concentration of mAb was able to be produced by the ST bioreactor (0.42 g l−1) compared to that of the MiniPerm (0.37 g l−1) and T-Flask bioreactor (0.23 g l−1).

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Acknowledgement

This work was financially supported by the Faculty of Engineering, International Islamic University, Kuala Lumpur, Malaysia, under grant of Final Year Project for Department of Biotechnology Engineering.

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Authors and Affiliations

  1. Bioprocess Engineering Research Group, Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728, Kuala Lumpur, Malaysia

    Maizirwan Mel, Abdul Rafiz Abdul Rahman, Mohamad Ramlan Mohamed Salleh & Yumi Zuhanis Has-Yun Hashim

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  1. Maizirwan Mel
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  2. Abdul Rafiz Abdul Rahman
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  3. Mohamad Ramlan Mohamed Salleh
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  4. Yumi Zuhanis Has-Yun Hashim
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Correspondence to Maizirwan Mel.

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Mel, M., Abdul Rahman, A.R., Mohamed Salleh, M.R. et al. Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor. World J Microbiol Biotechnol 24, 1923–1927 (2008). https://doi.org/10.1007/s11274-008-9696-4

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  • DOI: https://doi.org/10.1007/s11274-008-9696-4

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