Abstract
The co-culture of Bacillus subtilis IFO 3335 with Rhizopus peka P8 or Rhizopus oligosporus P12 in liquid medium was found to increase production of antibiotic activity and to alter the spectrum of activity relative to the pure cultures. However, a mixed culture of Rhizopus arrhizus P7 and Rhizopus oryzae P17 did not produce antibiotic activity. The concentration, ratio, and time of addition of B. subtilis to the R. peka culture was found to influence antibiotic yields. Solid-state fermentations using mixed cultures of R. peka and B. subtilis were investigated. The growth of Escherichia coli IFO 3792 as a target bacterium was inhibited by the mixed culture. These results suggest the possibility of biopreservation of fermented foods by novel co-culture systems.
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The authors thank Kaori Morita, Kazuhiro Noguche, Ying Wang, and Kana Morita for technical assistance.
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Fukuda, T., Yamamoto, S. & Morita, H. Changes in the antibiotic production by co-culture of Rhizopus peka P8 and Bacillus subtilis IFO3335. World J Microbiol Biotechnol 24, 1893–1899 (2008). https://doi.org/10.1007/s11274-008-9690-x
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DOI: https://doi.org/10.1007/s11274-008-9690-x