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Purification of lignin peroxidase isoforms from Streptomyces viridosporus T7A by hydrophobic based chromatographies

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Abstract

Five lignin peroxidases ALiP-P3 isoenzymes from Streptomyces viridosporus T7A were separated by hydrophobic interaction chromatography. The most abundant isoenzyme was purified by reversed phase chromatography and it was analyzed by mass spectrometry.

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Authors and Affiliations

  1. Instituto de Química, Departamento de Bioquímica, UFRJ, Rio de Janeiro, RJ, Brasil

    H. J. Nascimento & J. G. Silva Jr

  2. VDTEC, Instituto de tecnologia em Imunoreativos, Bio-Manguinhos, Fundação Oswaldo Cruz, Av. Brasil 4365, Pavilhão Rocha Lima, CEP 21040-900, Rio de Janeiro, RJ, Brasil

    H. J. Nascimento & J. G. Silva Jr

Authors
  1. H. J. Nascimento
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  2. J. G. Silva Jr
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Correspondence to J. G. Silva Jr.

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Nascimento, H.J., Silva, J.G. Purification of lignin peroxidase isoforms from Streptomyces viridosporus T7A by hydrophobic based chromatographies. World J Microbiol Biotechnol 24, 1973–1975 (2008). https://doi.org/10.1007/s11274-008-9688-4

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  • DOI: https://doi.org/10.1007/s11274-008-9688-4

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