Skip to main content
Springer Nature Link
Log in

Plant growth-promoting rhizobacteria for improving nodulation and nitrogen fixation in the common bean (Phaseolus vulgaris L.)

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

A greenhouse experiment was performed to evaluate the effects of plant growth-promoting rhizobacteria (PGPR) on nodulation, biological nitrogen fixation (BNF) and growth of the common bean (Phaseolus vulgaris L. cv. Tenderlake). Single and dual inoculation treatments of bean with Rhizobium and/or PGPR were administered to detect possible changes in the levels of and interactions between the phytohormones IAA and cytokinin. Bean plants cv. Tenderlake were grown in pots containing Fluvic Neosol eutrophic (pH 6.5). Fourteen kilogram aliquots of soil contained in 15-l pots were autoclaved. Bean seeds were surface sterilized and inoculated with Rhizobium tropici (CIAT 899-standard strain) alone and in combination with one of the PGPR strains: Bacillus endophyticus (DSM 13796), B. pumilus (DSM 27), B. subtilis (DSM 704), Paenibacillus lautus (DSM 13411), P. macerans (DSM 24), P. polymyxa (DSM 36), P. polymyxa (Loutit L.) or Bacillus sp.(65E180). The experimental design was randomized block design with three replications. Beans co-inoculated with Rhizobium tropici (CIAT899) and Paenibacillus polymyxa (DSM 36) had higher leghemoglobin concentrations, nitrogenase activity and N2 fixation efficiency and thereby formed associations of greater symbiotic efficiency. Inoculation with Rhizobium and P. polymyxa strain Loutit (L) stimulated nodulation as well as nitrogen fixation. PGPR also stimulated specific-nodulation (number of nodules per gram of root dry weight) increases that translated into higher levels of accumulated nitrogen. The activities of phytohormones depended on their content and interactions with Rhizobium tropici and Paenibacillus and/or Bacillus (PGPR) strains which affect the cytokinin in content in the common bean.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Explore related subjects

Discover the latest articles, books and news in related subjects, suggested using machine learning.

References

  • Amara MAT, Dahdoh MSA (1997) Effect of inoculation with plant growth-promoting rhizobacteria (PGPR) on yield and uptake of nutrients by wheat grown on sandy soil. Egypt J Soil 37:467–484

    Google Scholar 

  • Asghar HN, Zahir ZA, Arshad M, Khalig A (2002) Plant growth regulating substances in the rhizozphere: microbial production and functions. Adv Agron 62:146–151

    Google Scholar 

  • Bashan Y (1998) Inoculants of plant growth-promoting bacteria for use in agriculture. Biotechnol Adv 16:729–770

    Article  CAS  Google Scholar 

  • Bin L, Smith DL, Ping-Qui F (2000) Application and mechanism of silicate bacteria in agriculture and industry. Guizhou Sci 18:43–53

    Google Scholar 

  • Biswas JC, Ladha JK, Dazzo FB (2000) Rhizobial inoculation influences seedling vigor and yield of rice. Agron J 92:880–886

    Article  Google Scholar 

  • Boddey LH, Hungria M (1994) Classificação de estirpes de Bradyrhizobium japonicum em genótipo I e II baseada nas características fenotípicas e genotípicas. In: Simpósio Brasileiro Sobre Microbiologia de Solo, Londrina. Resumos. Londrina: LAPAR, p 66

  • Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–252

    Article  CAS  Google Scholar 

  • Bremner JM (1965) Total nitrogen. In: Black CA (ed) Methods of soil analysis chemical and microbiological properties. Part-2. Agronomy 9. American Society of Agronomy, Madison, pp 1149–1178, ASIN: B000H94EHO

    Google Scholar 

  • Camacho M et al (2001) Co-inoculation with Bacillus sp CECT 450 improves nodulation in Phaseolus vulgaris. Can J Microbiol 47:1058–1062

    Article  CAS  Google Scholar 

  • Cattelan AJ, Hartel PG, Fuhrmann JJ (1999) Screening for plant growth-promoting rhizobacteria to promote early soybean growth. Soil Sci Soc Am J 63:1670–1680

    Article  CAS  Google Scholar 

  • Chanway CP (1998) Bacterial endophytes: ecological and practical implications. Sydowia 50:149–170

    Google Scholar 

  • Chen C, Bauske EM, Musson G, Rodriguez-Kabaña R, Kloepper JW (1994). Biological control of Fusarium on cotton by use of endophytic bacteria. Biol Control 5:83–91

    Article  Google Scholar 

  • Cooper JB, Long SR (1994) Morphogenetic rescue of Rhizobium meliloti nodulation mutants by trans-zeatin secretion. Plant Cell 6:215–225

    Article  CAS  Google Scholar 

  • EMBRAPA (Empresa Brasileira de Pesquisa Agropecuária. Centro Nacional de Pesquisa em Solos) (1997) Manual de Métodos de Análise de Solos. Rio de Janeiro, 1997, v.2

  • Evans ML (1984) Functions of hormones at the cellular level of organization. In: Scott TK (ed) Hormonal regulation of development, II. Springer-Verlag, Berlin, pp 23–62, ISBN-10:0387101969

    Google Scholar 

  • Ferguson BJ, Mathesius U (2003) Signaling interactions during nodule development. J Plant Growth Regul 22:47–72

    Article  CAS  Google Scholar 

  • Ghosh S et al (2003) Three newly isolated plant growth-promoting bacilli facilitate the seedling growth of canola, Brassica campestris. Plant Physiol Biochem 41:277–281

    Article  CAS  Google Scholar 

  • Glick BR (1995) The enhancement of plant-growth by free-living bacteria. Can J Microbiol 41:109–117

    CAS  Google Scholar 

  • Hoagland DR, Arnon DI (1950) The water culture method of growing plants without soil. University of California, Berkeley, 39 pp, ASIN B00088T A3W

  • Izumi K et al (1988) Levels of IAA, cytokinin, ABA and ethylene in rice plants as affected by giberellin biosynthesis inhibitor Unincazole-P. Plant Cell Physiol 29:97–104

    CAS  Google Scholar 

  • Kloepper JW, Schroth MN, Miller TD (1980) Effects of rhizosphere colonization by plant growth-promoting rhizobacteria on potato plant development and yield. Phytopathology 70:1078–1082

    Article  Google Scholar 

  • Lambrecht M, Okon Y, Vande Broek A, Vanderleyden J (2000) Indole-3-acetic acid: a reciprocal signaling molecule in bacteria-plant interactions. Trends Microbiol 8:298–300

    Article  CAS  Google Scholar 

  • Lorteau MA, Ferguson BJ, Guinel FC (2001) Effects of cytokinin on ethylene production and nodulation in pea (Pisum sativum) cv.Sparkle. Physiol Plant 112:421–428

    Article  CAS  Google Scholar 

  • Nefedieva EE (2003) The influence of impulse pressure on the phytohormone content, growth and crop productivity of buckwheat plants (Fagopyrum esculentum Moench. cv. Aromat). Pac J Sci Technol 3:123–135

    Google Scholar 

  • Pan B, Bai YM, Leibovitch S, Smith DL (1999) Plant growth-promoting rhizobacteria and kinetin as ways to promote corn growth and yield in short season areas. Eur J Agron v 11:179–186

    Article  CAS  Google Scholar 

  • Rosendahl L, Jochimsen BV (1995) Uptake of indolacetic acid in symbiosomes from soybean (Glycine max L.) root nodules. In: Nitrogen fixation: fundamentals and applications. Proceedings. 10. International Congress of Nitrogen Fixation, 11, St.Petersburg. Resumos. St. Petersburg, Russian Academiy of Sciences, Russia, p. 336

  • Silva VN, Silva LESF, Figueiredo MVB (2006) Atuação de rizóbios com rizobactérias promotora de crescimento em plantas na cultura do caupi (Vigna unguiculata L. Walp). Acta Sci Agron 28:407–412

    Google Scholar 

  • Srinivasan M, Petersen DJ, Holl FB (1996) Influence of indoleacetic-acid-producing Bacillus isolates on the nodulation of Phaseolus vulgaris by Rhizobium etli under gnotobiotic conditions. Can J Microbiol 42:1006–1014

    Article  CAS  Google Scholar 

  • Timmusk S, Nicander B, Granhall U, Tillberg E (1999) Cytokinin production by Paenibacillus polymyxa. Soil Biol Biochem 31:1847–1852

    Article  CAS  Google Scholar 

  • Vessey JK (2003) Plant growth-promoting rhizobacteria as biofertilizers. Plant Soil 255:571–586

    Article  CAS  Google Scholar 

  • Wilson DO, Reisenauer HM (1963) Determination of leghemoglobin in legumes nodules. Anal Biochem 6:27–30

    Article  CAS  Google Scholar 

  • Yurekli F, Turkan I, Banu Porgali Z, Topcuoglu SF (2001) Indoleacetic acid, gibberellic acid, zeatin and abscisic acid levels in NaCl-treatment tomato species differing in salt tolerance. Isr J Plant Sci 49:269–277

    Article  CAS  Google Scholar 

  • Zhang F et al (1996) Plant growth-promoting rhizobacteria and soybean [Glycine max (L.) Merr.]. Nodulation and fixation at suboptimal root zone temperatures. Ann Bot 7:453–459

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) of Brazil and the University of British Columbia (UBC) of Canada for financial support.

Author information

Authors and Affiliations

  1. Laboratório de Biologia de Solo, IPA/EMBRAPA, CEP 50761-000, Recife, PE, Brazil

    M. V. B. Figueiredo & H. A. Burity

  2. Rua Sá e Souza, 1020/1401, CEP 51030-065, Boa Viagem, Recife, PE, Brazil

    M. V. B. Figueiredo

  3. Departamento de Biologia Molecular, Universidade Federal da Paraíba, Joao Pessoa, PB, Brazil

    C. R. Martinez

  4. Department of Forest Sciences, UBC, 3041-2424 Main Mall, Vancouver, Canada, V6T 1Z4

    C. P. Chanway

Authors
  1. M. V. B. Figueiredo
    View author publications

    Search author on:PubMed Google Scholar

  2. C. R. Martinez
    View author publications

    Search author on:PubMed Google Scholar

  3. H. A. Burity
    View author publications

    Search author on:PubMed Google Scholar

  4. C. P. Chanway
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to M. V. B. Figueiredo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Figueiredo, M.V.B., Martinez, C.R., Burity, H.A. et al. Plant growth-promoting rhizobacteria for improving nodulation and nitrogen fixation in the common bean (Phaseolus vulgaris L.). World J Microbiol Biotechnol 24, 1187–1193 (2008). https://doi.org/10.1007/s11274-007-9591-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-007-9591-4

Keywords