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Spoilage yeasts from Patagonian cellars: characterization and potential biocontrol based on killer interactions

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Indigenous yeasts associated with surfaces in three North Patagonian cellars were isolated by means of selective media developed for the isolation of Dekkera/Brettanomyces yeasts; 81 isolates were identified as belonging to Candida boidinii (16%), Hanseniaspora uvarum (38%), Pichia guilliermondii (3%), Saccharomyces cerevisiae (1%), Geotrichum silvicola (16%) and the new yeast species Candida patagonica (26%). No Dekkera/Brettanomyces isolate was obtained, however, 41 isolates (51% of the total isolates) produced some enologically undesirable features under laboratory conditions including the production of 4-ethylphenol and 4-vinylphenol, observed in the Candida boidinii and Pichia guilliermondii isolates. The sensitivity of the 41 spoilage isolates and seven Brettanomyces bruxellensis collection strains was evaluated against a panel of 55 indigenous and ten reference killer yeasts. Killer cultures belonging to Pichia anomala and Kluyveromyces lactis species showed the broadest killer spectrum against spoilage yeasts, including Dekkera bruxellensis collection strains. These killer isolates could be good candidates for use in biocontrol of regionally relevant spoilage yeasts.

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This work was supported by Universidad Nacional del Comahue project I-117, CONICET Grant Res.1104/04 and PIP 6494. We are grateful to UVEG and CSIC for kindly supplying online yeast database access (

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Correspondence to Adriana C. Caballero.

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Sangorrín, M.P., Lopes, C.A., Jofré, V. et al. Spoilage yeasts from Patagonian cellars: characterization and potential biocontrol based on killer interactions. World J Microbiol Biotechnol 24, 945–953 (2008).

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