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Spoilage yeasts from Patagonian cellars: characterization and potential biocontrol based on killer interactions

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Abstract

Indigenous yeasts associated with surfaces in three North Patagonian cellars were isolated by means of selective media developed for the isolation of Dekkera/Brettanomyces yeasts; 81 isolates were identified as belonging to Candida boidinii (16%), Hanseniaspora uvarum (38%), Pichia guilliermondii (3%), Saccharomyces cerevisiae (1%), Geotrichum silvicola (16%) and the new yeast species Candida patagonica (26%). No Dekkera/Brettanomyces isolate was obtained, however, 41 isolates (51% of the total isolates) produced some enologically undesirable features under laboratory conditions including the production of 4-ethylphenol and 4-vinylphenol, observed in the Candida boidinii and Pichia guilliermondii isolates. The sensitivity of the 41 spoilage isolates and seven Brettanomyces bruxellensis collection strains was evaluated against a panel of 55 indigenous and ten reference killer yeasts. Killer cultures belonging to Pichia anomala and Kluyveromyces lactis species showed the broadest killer spectrum against spoilage yeasts, including Dekkera bruxellensis collection strains. These killer isolates could be good candidates for use in biocontrol of regionally relevant spoilage yeasts.

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References

  • Barata A, Nobre A, Correia P, Malfeito-Ferreira M, Loureiro V (2006) Growth and 4-ethylphenol production by the yeast Pichia guilliermondii in grape juices. Am J Enol Viticult 57:133–138

    CAS  Google Scholar 

  • Ciani M, Fatichenti F (2001) Killer toxin of Kluyveromyces phaffii DBVPG 6076 as a biopreservative agent to control apiculate wine yeasts. Appl Environ Microbiol 67:3058–3063

    Article  CAS  Google Scholar 

  • Ciani M, Maccarelli F (1998) Oenological properties of non-Saccharomyces yeasts associated with wine-making. World J Microbiol Biotechnol 14:199–203

    Article  CAS  Google Scholar 

  • Comitini F, Ingeniis J, Pepe L, Mannazzu L, Ciani M (2004) Pichia anomala and Kluyveromyces wickerhamii killer toxins as new tools against Dekkera/ Brettanomyces spoilage yeasts. FEMS Microbiol Lett 238:235–240

    Article  CAS  Google Scholar 

  • Dias L, Pereira-da-Silva S, Tavares M, Malfeito-Ferreira M, Loureiro V (2003) Factors affecting the production of 4-ethylphenol by the yeast Dekkera bruxellensis in enological conditions. Food Microbiol 20:377–384

    Article  CAS  Google Scholar 

  • Du Toit M, Pretorius IS (2000) Microbial spoilage and preservation of wine: using weapons from nature´s own arsenal: A review. S Afr J Enol Viticult 21:74–96

    CAS  Google Scholar 

  • Esteve-Zarzoso B, Belloch C, Uruburu F, Querol A (1999) Identification of yeast by RFLP analysis of the 58S rRNA gene and the two ribosomal internal transcribed spacer. Int J Syst Bacteriol 49:329–337

    Article  CAS  Google Scholar 

  • Fleet GH (1993) The microorganims of winemaking: isolation, enumeration and identification. In: Fleet GH (ed) Wine microbiology and biotechnology. Harwood Academic Publishers, Switzerland, pp 1–25

    Google Scholar 

  • Fleet GH (1998) Microbiology of alcoholic beverages. In: Wood BJ (ed) Microbiology of fermented foods, Vol. I, 2nd edn. Blackie Academic & Professional, London, pp 217–262

    Google Scholar 

  • Kurtzman CP, Fell JW (1998) The yeasts a taxonomic study, 4th edn. Elseiver Sci Publ, Amsterdam, 1055 p

    Google Scholar 

  • Kurtzman CP, Robnett CJ (1998) Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73:331–371

    Article  CAS  Google Scholar 

  • Lopes CA, Rodríguez ME, Sangorrín MP, Querol A, Caballero AC (2007) Patagonian wines: implantation of an indigenous yeast starter in traditional and modern cellars. J Indus Microbiol Biotechnol 34:139–149

    Article  CAS  Google Scholar 

  • Loureiro V, Malfeito-Ferreira M (2003) Spoilage yeasts in the wine industry. Int J Food Microbiol 86:23–50

    Article  CAS  Google Scholar 

  • Magliani W, Conti S, Gerloni M, Bertolotti D, Polonelli L (1997) Yeast killer systems. Clin Microbiol Rev 3:369–400

    Google Scholar 

  • Martorell P, Barata A, Malfeito-Ferreira M, Fernández-Espinar MT, Loureiro V, Querol A (2006) Molecular typing of the yeast species Dekkera bruxellensis and Pichia guilliermondii recovered from wine related sources. Int J Food Microbiol 106:79–84

    Article  CAS  Google Scholar 

  • Sangorrín MP, Zajonskovsky I, Lopes CA, Rodríguez ME, van Broock MR, Caballero AC (2001) Killer behaviour in wild wine yeasts associated with Merlot and Malbec type musts spontaneously fermented from Northwestern Patagonia (Argentina). J Basic Microbiol 41:105–113

    Article  Google Scholar 

  • Sangorrín MP, Lopes CA, Giraudo MR, Caballero AC (2007a) Diversity and killer behaviour of indigenous yeasts isolated from the fermentation vats surfaces in four patagonian wineries. Int J Food Microbiol (in press DOI 10.1016/ j.ijfoodmicro.2007.04.010)

  • Sangorrín MP, Lopes CA, Belloch C, Querol A, Caballero AC (2007b) Candida patagonica sp.nov. a new yeast species from cellars surfaces. Antonie van Leeuwenhoeck 92:77–81

    Article  Google Scholar 

  • Santos A, Marquina D, Leal JA, Peinado JM (2000) (1→6)-β-D-Glucan as cell wall receptor for Pichia membranifaciens killer toxin. Appl Environ Microbiol 66:1809–1813

    Article  CAS  Google Scholar 

  • Schmitt MJ, Breinig F (2002) The viral killer system in yeast: from molecular biology to application. FEMS Microbiol Rev 26:257–276

    Article  CAS  Google Scholar 

  • Stratford M (2006) Food and beverage spoilage yeast. In: Querol A, Feet G (eds) Yeasts in food and beverages. Springer-Velarg Berlin Heidelberg, Germany

    Google Scholar 

  • Passoth V, Fredlund E, Druvefors UA, Schnürer J (2006) Biotechnology, physiology and genetics of the yeast Pichia anomala. FEMS Yeast Res 6:3–13

    Article  CAS  Google Scholar 

  • Torija MJ, Rozès N, Poblet M, Guillamón JM, Mas A (2001) Yeast population dynamics in spontaneous fermentation: comparison between two different wine-producing areas over a period of three years. Antonie van Leeuwenhoek 79:345–352

    Article  CAS  Google Scholar 

  • Ubeda JF, Briones AI (1999) Microbiological quality control of filtered and non-filtered wines. Food Control 10:41–45

    Article  Google Scholar 

  • van Keulen H, Lindmark DG, Zeman KE, Gerlosky W (2003) Yeasts present during spontaneous fermentation of Lake Erie Chardonnay Pinot Gris and Riesling. Antonie van Leeuwenhoeck 83:149–154

    Article  Google Scholar 

  • Zohre DE, Erten H (2002) The influence of Kloeckera apiculata and Candida pulcherrima yeasts on wine fermentation. Process Biochem 38:319–324

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by Universidad Nacional del Comahue project I-117, CONICET Grant Res.1104/04 and PIP 6494. We are grateful to UVEG and CSIC for kindly supplying online yeast database access (http://www.yeast-id.com).

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Authors and Affiliations

  1. Departamento de Química, Laboratorio de Microbiología y Biotecnología, Facultad de Ingeniería, Universidad Nacional del Comahue, Buenos Aires, 1400 (8300), Neuquén, Argentina

    Marcela P. Sangorrín, Christian A. Lopes & Adriana C. Caballero

  2. Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina (CONICET), Chubut , Argentina

    Marcela P. Sangorrín & Christian A. Lopes

  3. Departamento de Biología Aplicada, Laboratorio de Microbiología Agrícola, Facultad de Ciencias Agrarias, Universidad Nacional del Comahue, Cinco Saltos, Río Negro, Argentina

    Christian A. Lopes

  4. Laboratorio de Aromas y Sustancias Naturales, Luján de Cuyo, EEA Mendoza INTA, Mendoza, Argentina

    Viviana Jofré

  5. Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (C.S.I.C.), Paterna, Valencia, Spain

    Amparo Querol

Authors
  1. Marcela P. Sangorrín
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  2. Christian A. Lopes
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  3. Viviana Jofré
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  4. Amparo Querol
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  5. Adriana C. Caballero
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Correspondence to Adriana C. Caballero.

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Sangorrín, M.P., Lopes, C.A., Jofré, V. et al. Spoilage yeasts from Patagonian cellars: characterization and potential biocontrol based on killer interactions. World J Microbiol Biotechnol 24, 945–953 (2008). https://doi.org/10.1007/s11274-007-9557-6

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  • DOI: https://doi.org/10.1007/s11274-007-9557-6

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