Taxol, an anticancer drug produced by an endophytic fungus Bartalinia robillardoides Tassi, isolated from a medicinal plant, Aegle marmelos Correa ex Roxb.

  • V. Gangadevi
  • J. MuthumaryEmail author
Original Paper


Taxol is an important anticancer drug widely used in the clinic. An endophytic fungus Bartalinia robillardoides (strain AMB-9) was isolated from Aegle marmelos, a medicinal plant and screened for taxol production. The fungus was identified based on the morphology of the fungal culture and the characteristics of the spores. This fungus was grown in MID liquid medium and analyzed chromatographically and spectrometrically, for the presence of Taxol. The amount of taxol produced by this endophytic fungus was quantified by HPLC. It produced 187.6 μg/L of taxol which suggests that the fungus can serve as a potential material for genetic engineering to improve the production of Taxol. This fungal taxol isolated from the organic extract of this fungal culture, has strong cytotoxic activity towards BT 220, H116, Int 407, HL 251 and HLK 210 human cancer cells in vitro, tested by Apoptotic assay.


Fungal endophyte Medicinal plant Taxol Anticancer drug 



We thank Dr. N. Anand, Director, CAS in Botany, University of Madras for the laboratory facilities provided. One of the author (Gangadevi) is thankful to the Ministry of Environment and Forests, Government of India (Project No.14–53/2001-ERS-RE dt. 9.12.2003) for the Junior Research Fellowship during which the investigation was carried out.


  1. Cardellina JH (1991) HPLC separation of taxol and cephalomannine. J Chromatogr 14:659–665Google Scholar
  2. Chmurny GN, Hilton BD, Brobst S, Look SA, Witherup KN, Beutler JA (1992) 1H- and 13C-NMR assignments for Taxol, 7-epi-Taxol and Cephalomannine. J Nat Prod 55:414–423CrossRefGoogle Scholar
  3. Dobranic JK, Johnson JA, Alikhan QR (1995) Isolation of endophytic fungi from eastern larch (Larix laricina) leaves from New Brunswick, Canada. Can J Microbiol 41:194–198CrossRefGoogle Scholar
  4. Dreyfuss MM, Chapela IH (1994) Potential of fungi in the discovery of novel, low-molecular weight pharmaceuticals. In: Gullo VP (ed) The discovery of natural products with therapeutic potential. Butterworth-Heinemann, London, pp 49–80Google Scholar
  5. McClure TD, Schram KH (1992) The mass spectrometry of taxol. J Am Soc Mass Spectrom 3:672–679CrossRefGoogle Scholar
  6. Petrini O, Sieber TN, Toti L, Viret O (1992) Ecology, metabolite production and substrate utilization in endophytic fungi. Nat Toxins 1:185–196CrossRefGoogle Scholar
  7. Pinkerton R, Strobel G (1976) Serinol as an activator of toxin production in attenuated cultures of H. sacchari. Proc Nat Acad Sci USA 73:4007–4011CrossRefGoogle Scholar
  8. Ruckdeschel K, Roggenkamp A, Lafont V, Mangeat P, Hessmann J, Rouot B (1997) Interaction of Yersinia enterocolitica with macrophages leads to macrophage cell death through apoptosis. Infect Immun 65:4813–4821Google Scholar
  9. Schiff PB, Fant J, Horowitz SB (1979) Promotion of microtubule assembly in vitro by taxol. Nature 277:665–667CrossRefGoogle Scholar
  10. Stierle A, Stierle D, Strobel G, Bignami G, Gothaus P (1994) Bioactive metabolites of the endophytic fungi of Pacific yew, Taxus brevifolia. In: Georg GI, Chen TT, Ojima I, Vyas DM (eds) Taxane anticancer agents (Basic Sci Current Status, American Chemical Society Symposium Series no.583) Washington DC American Chemical Society pp. 81–97Google Scholar
  11. Stierle A, Strobel GA, Stierle D (1993) Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific Yew. Science 260:214–216CrossRefGoogle Scholar
  12. Strobel GA (2003) Endophytes as sources of bioactive products. Microbes Infect 5:535–544CrossRefGoogle Scholar
  13. Strobel GA, Yang X, Sears J, Kramer R, Sidhu RS, Hess WM (1996) Taxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallachiana. Microbiol 142:435–440CrossRefGoogle Scholar
  14. Yeung TK, Germond C, Chen X, Wang Z (1999) The mode of action of taxol: apoptosis at low concentration and necrosis at high concentration. Biochem Biophy Res Comm 263:398–404CrossRefGoogle Scholar
  15. Wani MC, Taylor HL, Wall ME, Coggon P, McPhail AT (1971). Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. J Am Chem Soc 93:2325–2327CrossRefGoogle Scholar

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© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Centre for Advanced Studies in BotanyUniversity of MadrasChennaiIndia

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