Taxol, an anticancer drug produced by an endophytic fungus Bartalinia robillardoides Tassi, isolated from a medicinal plant, Aegle marmelos Correa ex Roxb.
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Taxol is an important anticancer drug widely used in the clinic. An endophytic fungus Bartalinia robillardoides (strain AMB-9) was isolated from Aegle marmelos, a medicinal plant and screened for taxol production. The fungus was identified based on the morphology of the fungal culture and the characteristics of the spores. This fungus was grown in MID liquid medium and analyzed chromatographically and spectrometrically, for the presence of Taxol. The amount of taxol produced by this endophytic fungus was quantified by HPLC. It produced 187.6 μg/L of taxol which suggests that the fungus can serve as a potential material for genetic engineering to improve the production of Taxol. This fungal taxol isolated from the organic extract of this fungal culture, has strong cytotoxic activity towards BT 220, H116, Int 407, HL 251 and HLK 210 human cancer cells in vitro, tested by Apoptotic assay.
KeywordsFungal endophyte Medicinal plant Taxol Anticancer drug
We thank Dr. N. Anand, Director, CAS in Botany, University of Madras for the laboratory facilities provided. One of the author (Gangadevi) is thankful to the Ministry of Environment and Forests, Government of India (Project No.14–53/2001-ERS-RE dt. 9.12.2003) for the Junior Research Fellowship during which the investigation was carried out.
- Cardellina JH (1991) HPLC separation of taxol and cephalomannine. J Chromatogr 14:659–665Google Scholar
- Dreyfuss MM, Chapela IH (1994) Potential of fungi in the discovery of novel, low-molecular weight pharmaceuticals. In: Gullo VP (ed) The discovery of natural products with therapeutic potential. Butterworth-Heinemann, London, pp 49–80Google Scholar
- Ruckdeschel K, Roggenkamp A, Lafont V, Mangeat P, Hessmann J, Rouot B (1997) Interaction of Yersinia enterocolitica with macrophages leads to macrophage cell death through apoptosis. Infect Immun 65:4813–4821Google Scholar
- Stierle A, Stierle D, Strobel G, Bignami G, Gothaus P (1994) Bioactive metabolites of the endophytic fungi of Pacific yew, Taxus brevifolia. In: Georg GI, Chen TT, Ojima I, Vyas DM (eds) Taxane anticancer agents (Basic Sci Current Status, American Chemical Society Symposium Series no.583) Washington DC American Chemical Society pp. 81–97Google Scholar