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Restriction analysis of the amplified ribosomal DNA spacers ITS1 and ITS2 of Bipolaris sorokiniana isolates

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Abstract

The purpose of this study was to examine the genotypic variability of Bipolaris sorokiniana by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis using rDNA. Fifty B. sorokiniana isolates from Brazil and other countries, one Bipolaris oryzae and six Drechslera teres isolates were used. The intragenic spacer regions (ITS1 and ITS2) were the regions used for characterization of isolates. The amplification products for both ITS regions, showed two DNA fragments for all isolates. Two B. sorokiniana isolates presented an intraspecific variability showing a third fragment for the ITS1 region. The dendrograms generated with PCR-RFLP data showed intra- and inter-specific groups. The dendrograms showed that most of Brazilian isolates clustered together forming groups between them, and this behavior was repeated with most isolates from other countries. The dendrograms did not enable the separation of B. sorokiniana isolates by their geographic origin or host type. These results suggest the occurrence of gene flow between different populations of the fungus isolated in geographically distant regions and lends cogency to the occurrence of gene flow between species.

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Acknowledgments

We are grateful to Dr. Julie Nicol, Dr. Monica Mezzalama, Dr. Etienne Duveiller, and Dr. Henri Maraite from CIMMYT, Dr. Kerry O’Donnel from USDA for kindly provide the Bipolaris sorokiana samples from the other countries and to Dr. Airano Prestes from EMBRAPA for providing the B. sorokiniana isolates from barley and the wheat seeds. This study was financed by CAPES/PROF.

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  1. Departmento de Microbiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite 500, sala 158, Porto Alegre, RS, CEP 90050-170, Brazil

    Ernandes Joel de Moura Nascimento & Sueli Teresinha Van Der Sand

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  1. Ernandes Joel de Moura Nascimento
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  2. Sueli Teresinha Van Der Sand
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Correspondence to Sueli Teresinha Van Der Sand.

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de Moura Nascimento, E.J., Van Der Sand, S.T. Restriction analysis of the amplified ribosomal DNA spacers ITS1 and ITS2 of Bipolaris sorokiniana isolates. World J Microbiol Biotechnol 24, 647–652 (2008). https://doi.org/10.1007/s11274-007-9517-1

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  • DOI: https://doi.org/10.1007/s11274-007-9517-1

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