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Enhancement of fructanohydrolase synthesis from Aspergillus niger by simultaneous in vitro induction and in vivo acid stress using sucrose ester

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Abstract

This study describes a novel strategy to improve the fructanohydrolase production and growth performance of Aspergillus niger by the addition of sucrose ester to the culture medium in a 3 L fermenter. It was found that in the aerobic and non-pH-controlled condition, the sucrose ester not only acted as a more favorable in vitro inducer than inulin for fructanohydrolase formation, but also significantly forced in vivo carbon and energy flux into enzyme synthesis by acid stress. Response surface methodology (RSM) was used to optimize the composition of the culture medium, hence the fermentation period was shortened (near 50%) and enzyme activity was enhanced (over 4-fold higher), respectively. The results presented here provided a novel way to improve the inducible enzyme production by simultaneous inducement and acid stress.

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Southern Yangtze University, 1800# Lihu Road, Wuxi, JiangSu Province, 214122, P. R. China

    Xiang-Yang Ge & Wei-Guo Zhang

  2. School of Food Science, Southern Yangtze University, Wuxi, 214122, P. R. China

    He Qian

Authors
  1. Xiang-Yang Ge
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  2. He Qian
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  3. Wei-Guo Zhang
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Correspondence to Wei-Guo Zhang.

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Ge, XY., Qian, H. & Zhang, WG. Enhancement of fructanohydrolase synthesis from Aspergillus niger by simultaneous in vitro induction and in vivo acid stress using sucrose ester. World J Microbiol Biotechnol 24, 133–138 (2008). https://doi.org/10.1007/s11274-007-9450-3

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  • DOI: https://doi.org/10.1007/s11274-007-9450-3

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