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Isolation and characterization of bacteria from soil contaminated with diesel oil and the possible use of these in autochthonous bioaugmentation

World Journal of Microbiology and Biotechnology volume 23, pages 1739–1745 (2007)Cite this article

Abstract

Two bacterial species (isolates N and O) were isolated from a paddy soil microcosm that had been artificially contaminated with diesel oil to which extrinsic Pseudomonas aeruginosa strain WatG, had been added exogenously. One bacterial species (isolate J) was isolated from a similar soil microcosm that had been biostimulated with Luria–Bertani (LB) medium. Isolates N and O, which were tentatively identified as Stenotrophomonas sp. and Ochromonas sp., respectively, by sequencing of their 16 S rRNA genes had no ability to degrade diesel oil on their own in any liquid medium. When each strain was cocultivated with P. aeruginosa strain WatG in liquid mineral salts medium (MSM) containing 1% diesel oil, isolate N enhanced the degradation of diesel oil by P. aeruginosa strain WatG, but isolate O inhibited it. In contrast, isolate J, which was tentatively identified as a Rhodococcus sp., degraded diesel oil contained not only in liquid LB and MSM, but also in paddy soil microcosms supplemented with LB medium. The bioaugmentation capacity of isolate J in soil microcosms contaminated with diesel oil was much higher than that of P. aeruginosa strain WatG. The possibility of using isolate J for autochthonous bioaugmentation is discussed.

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Abbreviations

DGGE:

Denaturing gradient gel electrophoresis

LB:

Luria–Bertani medium

LB-BS:

LB-added biostimulation

MSM:

Mineral salts medium

WatG-BA:

P. aeruginosa strain WatG-added bioaugmentation

TPH:

Total petroleum hydrocarbons

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Acknowledgements

This work was partly supported by Grant-in-Aid for Scientific Research ((C) no. 17510061) from the Ministry of Education, Science, Sports, and Culture of Japan and grants from Northern Advancement Center for Science & Technology, the Sumitomo Foundation and Institute for Fermentation, Osaka (IFO).

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Author notes

  1. Akio Ueno

    Present address: ROM Co. Ltd., Chuo-ku, Sapporo, 064-0804, Japan

Affiliations

  1. Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo, 060-0810, Japan

    Akio Ueno & Hidetoshi Okuyama

  2. ROM Co. Ltd., Chuo-ku, Sapporo, 064-0804, Japan

    Yukiya Ito

  3. National Institute of Advanced Industrial Science and Technology (AIST), Toyohira-ku, Sapporo, 062-8517, Japan

    Isao Yumoto

Authors
  1. Akio Ueno
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  2. Yukiya Ito
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  3. Isao Yumoto
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  4. Hidetoshi Okuyama
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Corresponding author

Correspondence to Hidetoshi Okuyama.

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Ueno, A., Ito, Y., Yumoto, I. et al. Isolation and characterization of bacteria from soil contaminated with diesel oil and the possible use of these in autochthonous bioaugmentation. World J Microbiol Biotechnol 23, 1739–1745 (2007). https://doi.org/10.1007/s11274-007-9423-6

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  • DOI: https://doi.org/10.1007/s11274-007-9423-6

Keywords

  • Autochthonous bioaugmentation
  • Biostimulation
  • Diesel oil degradation
  • Soil intrinsic bacteria
  • Soil microcosms