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Interspecies variation of Kitasatospora recifensis endophytic from yam bean producing thermostable amylases in alternative media

Abstract

An endophytic actinomycete isolated from tubers of yam beam (Pachyrhizus erosus L. Urban) was classified as a novel species nominated Kitasatospora recifensis based in phenotypic and genotypic analysis (16S rDNA gene sequence). Monosporic culture using specific ISP2 media revealed three interspecies, which were identified by DNA southern hybridization (Wild strain 13817 W, Aerial Mycelium strain 13817 AM and Vegetative Mycelium strain 13817 VM). The strains were tested for the production of amylolitic enzymes in alternative media. Maximum yields for both enzymes were observed in starch-casein. Higher α-amylase was obtained with strain 13817 W in starch-urea, and amyloglucosidase with strain 13817 AM in starch-ammonium that are economic sources and may be important for industrial purposes. Type strain (DAUFPE 13817T = KCTC 9972= DSM 44943T).

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Acknowledgements

The authors are indebted to Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq, for the fellowships and to Fundação de Amparo à Pesquisa do Estado de Pernambuco (FACEPE) and the Korean Research Institute of Bioscience and Biotechnology (KRIBB) for research support.

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Correspondence to Tania Lucia Montenegro Stamford.

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Stamford, T.L.M., Stamford, T.C.M., Stamford, N.P. et al. Interspecies variation of Kitasatospora recifensis endophytic from yam bean producing thermostable amylases in alternative media. World J Microbiol Biotechnol 23, 1719–1724 (2007). https://doi.org/10.1007/s11274-007-9420-9

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  • DOI: https://doi.org/10.1007/s11274-007-9420-9

Keywords

  • 16S rDNA
  • Amylolitic enzymes
  • α-Amylase
  • Amyloglucosidase
  • Gene sequence
  • Pachyrhizus erosus