Skip to main content
SpringerLink
Log in

Interspecies variation of Kitasatospora recifensis endophytic from yam bean producing thermostable amylases in alternative media

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

An endophytic actinomycete isolated from tubers of yam beam (Pachyrhizus erosus L. Urban) was classified as a novel species nominated Kitasatospora recifensis based in phenotypic and genotypic analysis (16S rDNA gene sequence). Monosporic culture using specific ISP2 media revealed three interspecies, which were identified by DNA southern hybridization (Wild strain 13817 W, Aerial Mycelium strain 13817 AM and Vegetative Mycelium strain 13817 VM). The strains were tested for the production of amylolitic enzymes in alternative media. Maximum yields for both enzymes were observed in starch-casein. Higher α-amylase was obtained with strain 13817 W in starch-urea, and amyloglucosidase with strain 13817 AM in starch-ammonium that are economic sources and may be important for industrial purposes. Type strain (DAUFPE 13817T = KCTC 9972T = DSM 44943T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Bernfeld P (eds) (1995) Methods in enzymology. Academic Press Inc., New York, pp 149–150

    Google Scholar 

  • Castro RR, Ferreri MA, Abate BS, Mendez BS, Siñeriz F (1992) Simultaneous production of alpha and beta amylases by Bacillus subtilis MIR – 5 in batch and continuous culture. Biotechnol Let 14:49–54

    Article  CAS  Google Scholar 

  • Chung YR, Sung KC, Mo HK, Young son D, Nam JS, Chun J, Bae KS (1999) Kitasatospora cheerisanensis sp. nov., a new species of the genus Kitasatospora that produces an antifungal agent. Intern. J Syst Bacteriol 45:753–758

    Google Scholar 

  • Collins MD, Jones D (1981) Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 45:316–354

    CAS  Google Scholar 

  • Cullum J, Flett F, Piendl W (1989) Genetic Instability, deletions and DNA simplification in Streptomyces species. In: Genetics and molecular biology of industrial microorganisms. Amer. Soc. Microbiol. p 203

  • Groth I, Rodriguez Schütze B, Schmitz P, Leistner E, Goodfellow M (2004) Kitasatospora putterlickiae sp. nov., isolated from rhizosphere soil, transfer of Streptomyces kifunensis to the genus Kitastospora as Kitasatospora kifunensis comb. Nov., and emended description of Streptomyces aureofaciens Duggar 1948. Int J Syst Evol Microbiol 53:2033–2040

    Article  CAS  Google Scholar 

  • Hankin L, Anagnostakis SL (1975) The use of solid media for detection of enzyme production by fungi. Mycol 67:597–607

    Article  Google Scholar 

  • Hopwood DA, Bibb MJ, Chater KF, Kiese T, Gruton CJ, Kieser HM, Lydiate DJ, Smith CP, Ward JM, Schirempe H (1985) Genetic manipulation of Streptomyces a laboratory manual, John Innes Inst., p 302

  • Jacobson E, Granville WC, Foss CE (1948) Colour harmony manual, 3rd edn. Container Corp. Amer., p 58

  • Kelly CT, Hefferman ME, Fogarty WM (1980) A novel α-amyloglucosidase produced by Bacillus amylolyticus. Biotechnol Let 2:351–356

    Article  CAS  Google Scholar 

  • Küster E (1972) Simple working key for the classification and identification of named taxa included in the International Streptomyces Project. Intern J System Bacteriol 22:139–148

    Google Scholar 

  • Linko Y, Wu SY (1993) Improvement and estimation of enzymic starch saccharification process. Biotechnol Technol 7:551–556

    Article  CAS  Google Scholar 

  • Liu Z, Rodriguez CR, Wang L, Cui Q (2005) Kitasatospora viridis sp. nov., a novel actinomycete from soil. Int J Syst Evol Microbiol 55:707–711

    Article  CAS  Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folium phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  • McInroy JA, Kloepper JW (1991) Analysis of population sanities and identification of endophytic bacteria of maize and cotton in the field. Kew Bull 14:328–331

    Google Scholar 

  • Melo EA, Krieger N, Stamford TLM (1994) Physico-chemical properties of jacatupé (Pachyrhizus erosus L Urban). Starch 46:245–247

    Article  CAS  Google Scholar 

  • Melo EA, Vieira R, Krieger N, Guerra NB, Silva MPC, Kennedy JF (1996) Enzymatic hydrolysis of starch from jacatupé (Pachyrhizus erosus L Urban) by thermostable amylolitic enzymes. Starch 48:101–104

    Article  CAS  Google Scholar 

  • Nolan RD, Cross D (1988) Actinomycetes in biotechnology. Acad. Press. Inc., San Diego, California, p 204

    Google Scholar 

  • Omura S, Takahashi Y, Iwi Y, Tanaka H (1983) Kitasatospora setalba sp. nov. In Validation of the publication of new names and new combinations previously effectively published outside the IJSB, List no. 11. Int J Syst Bacteriol 33:672–674

    Google Scholar 

  • Pandey A, Nigam P, Soccol CR, Soccol VT, Singh D, Mohan R (2000) Advances in Microbial Amylases. Biotechnol Appl Biochem 31:135–152

    Article  CAS  Google Scholar 

  • Prauser H (1964) Aptness and application of colour codes for exact description of colour of Streptomyces. Zeitschrift Allag Mikrobiol 4:95–98

    Article  Google Scholar 

  • Pridham TG, Anderson P, Foley C, Lindenfelser LA, Hesseltine CW, Benedict RG (1956/57) A selection of media for maintenance and taxonomic study of Streptomyces. Antibiotics Ann, 947–953

  • Riddell RW (1950) Permanent stained mycological preparation obtained by slide culture. Mycol 42:265–270

    Article  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a Laboratory Manual, 2nd ed. Cold Spring Harbor, New York

    Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. DE MIDI Inc., Newark

    Google Scholar 

  • Shirling EB, Glottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Google Scholar 

  • Sorensen M (1996) Yam bean: Pachyrhizus D.C. International Plant Genetic Resources Institute, Rome p 141

    Google Scholar 

  • Souza AO (1996) Bactérias endofíticas de milho (Zea mays. L.) e sua variabilidade genética analisada por RAPD. Piracicaba, 85p. MSc., thesis. Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo

  • Stamford TLM, Araújo JM, Stamford NP (1998) Atividade enzimática de microrganismos isolados do jacatupé (Pachyrhizus erosus L. Urban). Ci Tecnol Alim 18:382–385

    CAS  Google Scholar 

  • Stamford TML, Stamford NP, Coelho LCB, Araújo JM (2002) Production and characterization of a thermostable amyloglucosidase from Streptosporangium sp. endophyte of maize leaves. Biores Technol 83:105–109

    Article  CAS  Google Scholar 

  • Staneck JL, Roberts GD (1974) Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 2:226–231

    Google Scholar 

  • Tamaoka J, Komagata K (1984) Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128

    Article  CAS  Google Scholar 

  • Timothy L, Mc Cutcheon N, Carrol GA (1993) Genotypic diversity in populations of a fungal endophyte from Douglas Fir. Mycol 82:180–186

    Google Scholar 

  • Vigal T, Gil JF, Daza A, Garcia-Gonzalez MD, Martin JF (1991) Cloning characterization and expresión o fan α amylae gene from Streptomyces griseus IMRU 3570. Mol Gen Genet 225:278–288

    Article  CAS  Google Scholar 

  • Yoon JH, Kang KH, Park YH (2003) Halobacillus salinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea. Int J Syst Evol Microbiol 53:687–693

    Article  CAS  Google Scholar 

  • Zhang Z, Wang Y, Ruan J (1997) A proposal to revive the genus Kitasatospora (Omura, Takahashi, Iwai and Tanaka, 198). Int J Syst Bacteriol 47:1048–1054

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors are indebted to Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq, for the fellowships and to Fundação de Amparo à Pesquisa do Estado de Pernambuco (FACEPE) and the Korean Research Institute of Bioscience and Biotechnology (KRIBB) for research support.

Author information

Authors and Affiliations

  1. Department of Nutrition, University Federal of Pernambuco-UFPE, Rua Jader de Andrade, 335 Casa Forte, 52061-060, Recife, Pernambuco, Brazil

    Tania Lucia Montenegro Stamford

  2. University Integrated of Patos, Paraíba, Brazil

    Thayza Christina Montenegro Stamford

  3. Department of Agronomy, University Federal Rural of Pernambuco-UFRPE, Recife, Brazil

    Newton Pereira Stamford, Carolina Etienne Rosália Silva Santos & Maria do Carmo Catanho Pereira de Lyra

  4. Korea Research Institute of Bioscience & Biotechnology, Dae Jeon, Korea

    Yong Ha-Park & Jin-Won Bae

  5. Department of Anitibiotics, University Federal of Pernambuco, Recife, Brazil

    Janete Magali Araújo

Authors
  1. Tania Lucia Montenegro Stamford
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Thayza Christina Montenegro Stamford
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Newton Pereira Stamford
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Carolina Etienne Rosália Silva Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Maria do Carmo Catanho Pereira de Lyra
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yong Ha-Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jin-Won Bae
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Janete Magali Araújo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tania Lucia Montenegro Stamford.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Stamford, T.L.M., Stamford, T.C.M., Stamford, N.P. et al. Interspecies variation of Kitasatospora recifensis endophytic from yam bean producing thermostable amylases in alternative media. World J Microbiol Biotechnol 23, 1719–1724 (2007). https://doi.org/10.1007/s11274-007-9420-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-007-9420-9

Keywords

Search

Navigation