Abstract
Tomato fruits and seed lots were screened for the presence of Xanthomonas vesicatoria and Ralstonia solanacearum. Yellow colonies of Xanthomonas vesicatoria and white colonies of Ralstonia solanacearum were consistently isolated on yeast extract-dextrose-calcium carbonate agar medium (YDC) from diseased fruits and seed samples. This was confirmed by isolation on semi-selective medium such as Tween B for Xanthomonas and triphenyltetrazolium salt (TTC) medium for Ralstonia solanacearum followed by biochemical tests. The four isolates belonging to Xanthomonas vesicatoria and Ralstonia solanacearum were used to inoculate a local tomato variety. The isolates were found to cause yellowing and wilting of 2-weeks-old seedlings by 8–14 days after inoculation and by 4 weeks all plants had wilted and completely died. Bacteria with the same characteristics as those inoculated were reisolated from the infected plants. Uninoculated plants remained healthy.
This is a preview of subscription content, access via your institution.



References
Adhikari TB (1993) Identification of biovars and races of Pseudomonas solanacearum and source of resistance in tomato in Nepal. Plant Dis 77:905–907
Black R, Seal S, Zakia Abubakar, Nono-Womdim R, Swai I (2001) Bacterial spot (Xanthomonas campestris pv. vesicatoria) of tomato and sweet pepper in Tanzania. New Disease Reports 3, 2001
Jones JB, Stall RE, Bouzar H (1998) Diversity among xanthomonads pathogenic on pepper and tomato. Ann Rev Phytopathol 36:41–58
Kelman A (1954) The relationship of pathogenicity in Pseudomonas solanacearum to colony appearance on a tetrazolium medium. Phytopathology 44:693–695
Mcguire RG, Jones JB, Sasser M (1986) Tween media for semiselective isolation of Xanthomonas campestris pv. vesicatoria from soil and plant material. Plant Dis 70:887–891
OEPP/EPPO (2004) Diagnostic protocols for regulated pests. Bulletin OEPP/EPPO Bulletin 34:173–178
Palleroni NJ (1984) Family I. Pseudomonadaceae. In: Krieg R, Holt JG (eds) Bergey’s manual of systemic bacteriology, vol. 1. Williams &Wilkins, London UK, pp 141–219
Sahin F (2001) Pepper races 7, 8 and 10 of Xanthomonas axonopodis pv. vesicatoria isolated from diseased pepper plants in Turkey. Plant Pathol 50:809
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Abd-Alla, M.H., bashandy, S.R. Bacterial wilt and spot of tomato caused by Xanthomonas vesicatoria and Ralstonia solanacearum in Egypt. World J Microbiol Biotechnol 24, 291–292 (2008). https://doi.org/10.1007/s11274-007-9385-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11274-007-9385-8