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Separation of yeasts by addition of flocculent cells of Saccharomyces cerevisiae

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Abstract

Separation of yeast cells using a co-flocculation process was investigated. Co-flocculation is a fast process (within few minutes), occurs in a broad pH range (3.0–8.0) and requires a small amount of calcium (0.1 mM). Agitation affects yeast aggregation; however, an agitation between 60 rev/min and 160 rev/min has only a little influence on the co-flocculation process. The ratio flocculent/non-flocculent cells that induced the settling of 50 and 90% of the cells of S. cerevisiae was 1:7 and 1:1, respectively. Separation of non-flocculent cells can be carried out at any time of the growth cycle. No difference in the efficiency of co-flocculation carried out in buffer (pH 4.0 with 10 mM calcium) or in 48 h-fermented broth was observed. Flocculent cells of Saccharomyces cerevisiae had the ability to sediment non-flocculent cells of S. cerevisiae and Kluyveromyces marxianus, which shows the suitability of the co-flocculation process for separation of different kinds of non-flocculent cells.

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Acknowledgements

Annelies Mortier (KaHo St.-Lieven, Gent, Belgium) wishes to thank Dr C. Van Keer, for the opportunity to participate in the ERASMUS bilateral agreement program between their school and ISEP (Portugal).

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Authors and Affiliations

  1. Department of Biochemistry-Microbiology, KaHo St.-Lieven, Industrial Engineering, Gebroeders Desmetstraat 1, 9000, Ghent, Belgium

    Annelies Mortier

  2. Chemical Engineering Department, Superior Institute of Engineering from Porto Polytechnic Institute, Rua Dr António Bernardino de Almeida, 431, 4200-072, Porto, Portugal

    Annelies Mortier & Eduardo V. Soares

  3. IBB-Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal

    Eduardo V. Soares

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  1. Annelies Mortier
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  2. Eduardo V. Soares
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Correspondence to Eduardo V. Soares.

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Mortier, A., Soares, E.V. Separation of yeasts by addition of flocculent cells of Saccharomyces cerevisiae . World J Microbiol Biotechnol 23, 1401–1407 (2007). https://doi.org/10.1007/s11274-007-9379-6

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  • DOI: https://doi.org/10.1007/s11274-007-9379-6

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