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Changes of chemical and nutrient composition of porcine blood during fermentation by Aspergillus oryzae

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Abstract

A solid-state fermentation (SSF) of a mixture of porcine blood and wheat bran with a ratio of 8:1 by Aspergillus oryzae was investigated. Water content, pH, crude protein, heme and total iron, free amino acids (FAA) and total fatty acids (TFA) of the fermented mixture were determined at 0, 40, 80 and 120 h, respectively, and protein hydrolysis were analyzed with SDS-PAGE accordingly. The results showed that, during the fermentation, water contents and pH decreased significantly (P < 0.05) from 68.33% to 59.94%, 7.18% to 4.48%, respectively. Heme iron content changed slightly (P > 0.05). With the degradation of large protein molecules, free amino acids in the mixture increased from 872.83 mg l−1 to 11560.94 mg l−1 (P < 0.05). Content of free isoleucine, methionine and cystine, deficient in fresh porcine blood increased (P < 0.05) after fermentation. Percentages of saturated fatty acids such as C14:0, C17:0 and C18:0 in the total fatty acids decreased significantly (P < 0.05), and those of polyunsaturated fatty acids C18:1, C18:2, C18:3, C20:1 and C20:2 increased significantly from 27.06% to 47.90% (P < 0.05). The results indicated that Aspergillus oryzae could ferment porcine blood and bran mixture and change its chemical and nutrient composition.

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References

  • Abu OA, Tewe OO, Losel DM, Onifade AA (2000) Changes in lipid, fatty acids and protein composition of sweet potato (Ipomoea batatas) after solid-state fungal fermentation. Biores Technol 72:189–192

    Article  CAS  Google Scholar 

  • AOAC (1990) Official methods of analysis, 15th edn. Association of Official Analytical Chemists, Arlington, ISBN 0-93558-442-0

  • Bellon-Maurel V, Orliac O, Christen P (2003) Sensors and measurements in solid state fermentation: a review. Process Biochem 38:881–896

    Article  CAS  Google Scholar 

  • Calder PC, Newsholme EA (1992) Polyunsaturated fatty acids suppress human peripheral blood lymphocyte proliferation and interleukin-2 production. Clin Sci 82:695–700

    CAS  Google Scholar 

  • Clark EM, Mahoney AW, Carpenter CE (1997) Heme and total iron in ready-to-eat chicken. J Agric Food Chem 45:124–126

    Article  CAS  Google Scholar 

  • Donkoh A, Atuahene CC, Anang DM, Ofori SK (1999) Chemical composition of solar-dried blood meal and its effect on performance of broiler chickens. Animal Feed Sci Technol 81:299–307

    Article  CAS  Google Scholar 

  • Eriksson C (1982) Hemiron-enriched amino acid preparation and a process for the preparation of hemiron-enriched amino acid preparation from hemprotein. Europe Patent Application. No 61556

  • FAO/WHO/UNU (1985) Energy and protein requirements. World Health Organization, Geneva, ISBN 92-4-120724-8

    Google Scholar 

  • Feron G, Bonnarme P, Durand A (1996) Prospects for the microbial production of food flavors. Trends Food Sci Technol 7:285–293

    Article  CAS  Google Scholar 

  • Godel H, Graser T, Földi P, Pfaender P, Fürst P (1984) Measurement of free amino acids in human biological fluids by High-Performance Liquid Chromatography. J Chromatogr 49:49–61

    Article  Google Scholar 

  • Hornsey HC (1956) The color of cooked cured pork, I. Estimation of the nitric oxide-heme pigments. J Sci Food Agric 7:534–540

    Article  CAS  Google Scholar 

  • Kalpalathika PV, Clark EM, Mahoney AW (1991) Heme iron content in selected ready-to-serve-beef products. J Agric Food Chem 39:1091–1093

    Article  CAS  Google Scholar 

  • Kiers JL, Van Laeken AE, Rombouts FM, Nout MJ (2000) In vitro digestibility of bacillus fermented soya bean. Int J Food Microbiol 60:163–169

    Article  CAS  Google Scholar 

  • Kongkachuichai R, Napatthalung P, Charoensiri R (2002) Heme and nonheme iron content of animal products commonly consumed in Thailand. J Food Comp Anal 15:389–398

    Article  CAS  Google Scholar 

  • Leonhardt M, Wenk C (1997) Variability of selected vitamins and trace elements of different meat cuts. J Food Comp Anal 10:218–224

    Article  CAS  Google Scholar 

  • Mansbridge RJ, Blake JS (1997) Nutritional factors affecting the fatty acid composition of bovine milk. Br J Nutr 78:S37–S47

    Article  CAS  Google Scholar 

  • Marichal M de J, Carriquiry M, Pereda R, Martín RS (2000) Protein degradability and intestinal digestibility of blood meals: comparison of two processing methods. Animal Feed Sci Technol 88:91–101

    Article  Google Scholar 

  • Márquez E, Bracho M, Archile A, Rangel L, Benítez B (2005) Proteins, isoleucine, lysine and methionine content of bovine, porcine and poultry blood and their fractions. Food Chem 93:503–505

    Article  Google Scholar 

  • Matsumoto M, Kobayashi T, Itabashi H, ørskov ER (1995) Investigations of the protection of soybean meal and amino acids from rumen degradation with whole blood treatment. Animal Feed Sci Technol 56:37–43

    Article  CAS  Google Scholar 

  • Merck Index (1989) An encyclopedia of chemicals, drugs and biologicals, 11th edn. Rahway, Merck, ISBN 0-911910-28-1

  • M’ncenem WB, Tuitoek JK, Muiruri HK (1999) Nitrogen utilization and performance of pigs given diets containing a dried or undried fermented blood/molasses mixture. Animal Feed Sci Technol 78:239–247

    Article  Google Scholar 

  • Montel MC, Masson F, Talon R (1998) Bacterial role in flavor development. Meat Sci 49:S111–S123

    Article  CAS  Google Scholar 

  • Moughan PJ, Dong GZ, Pearson G, Wilkinson BHP (1999) Protein quality in blood meal. II. The effect of processing on in vivo nitrogen digestibility in rats, protein solubility and FDNB-available lysine. Animal Feed Sci Technol 79:309–320

    Article  CAS  Google Scholar 

  • Mudgett RE (1986) Solid state fermentation. In: Demain AL, Solomon NA (eds) Manual of industrial microbiology and biotechnology. American Society for Microbiology, Washington, ISBN 1-55581-128-0

    Google Scholar 

  • Ockerman HW, Hansen CL (2000) Animal by-product processing and utilization. Technomic Publishing, Lancaster, ISBN 1-566767-77-6

    Google Scholar 

  • SAS Institute (1999) Statistical analysis system release 8.01. SAS Institute, Cary

  • Stahnke LH, Holck AL, Jensen A, Nilsen A, Zanardi E (2002) Maturity acceleration by Staphylococcus carnosus in fermented sausage–relationship between maturity and flavor compounds. J Food Sci 67:1914–1921

    Article  CAS  Google Scholar 

  • Sukhija PS, Palmquist DL (1988) Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. J Agric Food Chem 36:1202–1206

    Article  CAS  Google Scholar 

  • Te Biesebeke R, Ruijter G, Rahardjo YS, Hoogschagen MJ, Heerikhuisen M, Levin A (2002) Aspergillus oryzae in solid-state and submerged fermentations progress report on a multi-disciplinary project. FEMS Yeast Res 2:245–248

    Article  CAS  Google Scholar 

  • Tomasini A, Fajardo C, Barrios-González J (1997) Gibberellic acid production using different solid-state fermentation systems. World J Microbiol Biotechnol 13:203–206

    Article  CAS  Google Scholar 

  • van der Aa BC, Asther M, Dufreûe YF (2002) Surface properties of Aspergillus oryzae spores investigated by atomic force microscopy. Colloids Surf B 24:277–284

    Article  Google Scholar 

  • Wang FS, Lin CW (1994) The effects of heating and chemical treatment on the heme and nonheme iron content of heat-induced porcine blood curd. J Sci Food Agric 65:209–213

    Article  CAS  Google Scholar 

  • Weete JD (1980) Lipid biochemistry of fungi and other organisms. Plenum Press, New York, ISBN 0-306405-70-9

    Google Scholar 

  • Yu YK, Hu JE, Bai XF, Du YG (2004) Progress on research of bioactive peptides derived from porcine blood. Fine and Special Chemical 12:10–13 (in Chinese)

    CAS  Google Scholar 

  • Zapelena MJ, AstiasaraÂn I, Bello J (1999) Dry fermented sausages made with a protease from Aspergillus oryzae and/or a starter culture. Meat Sci 52:403–409

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors gratefully acknowledge financial assistance from the 11th Five-Year Plan of the Ministry of Science and Technology of P.R. China (2006BAD05A16). The authors also thank Dr. S.S. Zeng (Langston University, Oklahoma, USA), Dr. Y.S.P. Rahardjo (Wageningen University, Wageningen, Netherlands), A. Hussain (Chemistry Department, Pakistan College, Beijing, China) and Y.J. Wang (Chinese Academy of Sciences, Beijing, China) for their help in revision for the manuscript.

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  1. College of Food Science & Nutritional Engineering, China Agricultural University, East Campus, Beijing, 100083, P.R. China

    J. Z. Wang, M. Zhang, F. Z. Ren, B. Z. Han, S. W. Chen & A. Humera

  2. Grain Processing Corp., Muscatine, IA, 52761, USA

    L. Wang

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  1. J. Z. Wang
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  2. M. Zhang
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Correspondence to F. Z. Ren.

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Wang, J.Z., Zhang, M., Ren, F.Z. et al. Changes of chemical and nutrient composition of porcine blood during fermentation by Aspergillus oryzae . World J Microbiol Biotechnol 23, 1393–1399 (2007). https://doi.org/10.1007/s11274-007-9378-7

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  • DOI: https://doi.org/10.1007/s11274-007-9378-7

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