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Enhanced production of lactic acid by an adapted strain of Lactobacillus delbrueckii subsp. lactis


Lactobacillus delbrueckii subsp. lactis strains were developed having increased activity, by gradually acclimatizing the bacteria to acidic conditions over repeated batch culture. Cells from one batch culture were used as the inoculum for the subsequent batch culture and thereby an adapted strain of Lactobacillus was obtained showing improved lactic acid productivity, cell growth and total glucose utilization. Furthermore, the acclimatized cells used significantly less nitrogen for a given level of lactic acid production, which is significant from an industrial point of view. The developed procedure decreases fermentation time and nutrient use, leading to reduced operation costs, while providing a lactic acid yield superior to previously reported methods.

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Correspondence to KiBeom Lee.

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Lee, K. Enhanced production of lactic acid by an adapted strain of Lactobacillus delbrueckii subsp. lactis . World J Microbiol Biotechnol 23, 1317–1320 (2007).

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  • Lactobacillus delbrueckii subsp. Lactis
  • Acclimatization
  • Partial reuse of culture
  • Subsequent batch culture
  • Lactic acid productivity