Abstract
Dye-decolorizing peroxidases (DyP) are atypical peroxidases showing no homology to other fungal peroxidases and lacking the typical heme binding region conserved among plant peroxidase superfamily. The gene and the corresponding cDNA encoding DyP from Pleurotus ostreatus have been identified on the basis of sequence homology analyses. The deduced amino acid sequence shares 43% identity with DyP from the ascomycete Thanatephorus cucumeris Dec 1. Analyses of the protein sequence by homology searches pointed out some properties of the DyP-type peroxidase family, which includes members from bacteria, ascomycete, and basidiomycete fungi. Some amino acids (C374, H379, and Y501 in the P. ostreatus DyP sequence) are proposed as candidates for the heme ligand, providing a basis for further investigations on the structure of the DyP type peroxidase family members.
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References
Conesa A, Punt PJ, van den Holden CA (2002) Fungal peroxidases: molecular aspects and applications. J Biotechnol 93:143–158
Giardina P, Cannio R, Martirani L, Marzullo L, Palmieri G, Sannia G (1995) Cloning and sequencing of a laccase gene from the lignin-degrading basidiomycete Pleurotus ostreatus. Appl Environ Microbiol 61:2408–2413
Giardina P, Palmieri G, Fontanella B, Rivieccio V, Sannia G (2000) Manganese peroxidase isoenzymes produced by Pleurotus ostreatus grown on wood sawdust. Arch Biochem Biophys 376:171–179
Johjima T, Ohkuma M, Kudo T (2003) Isolation and cDNA cloning of novel hydrogen peroxide-dependent phenol oxidase from the basidiomycete Termitomyces albuminosus. Appl Microbiol Biotechnol 61:220–225
Kersten PJ, Tien M, Kalyanaraman B, Kirk TK (1985) The ligninase of Phanerochaete chrysosporium generates cation radicals from methoxybenzenes. J Biol Chem 260:2609–2612
Kim SJ, Shoda M (1999) Purification and characterization of a novel peroxidase from Geotrichum candidum Dec 1 involved in decolorization of dyes. Appl Environ Microbiol 65:1029–1035
Kuwahara M, Glenn JK, Morgan MA, Gold MH (1984) Separation and characterization of two extracellular H2O2-dependent oxidases from ligninolytic cultures of Phanerochaete chrysosporium. FEBS Lett 169:247–250
Lucas MC, Jacobson JW, Giles NH (1977) Characterization and in vitro translation of polyadenylated messenger ribonucleic acid from Neurospora crassa. J Bacteriol 130:1192–1198
Palmieri G, Cennamo G, Faraco V, Amoresano A, Sannia G, Giardina P (2003) Atypical laccase isoenzymes from copper supplemented Pleurotus ostreatus cultures. Enzyme Microbial Technol 33:220–230
Palmieri G, Cennamo G, Sannia G (2005) Remazol Briliant Blue R decolorisation by the fungus Pleurotus ostreatus and its oxidative enzymatic system. Enzyme Microbial Technol 36:17–24
Pointing SB (2001) Feasibility of bioremediation by white-rot fungi. Appl Microbiol Biotechnol 57:20–33
Ruiz-Duenas FJ, Camarero S, Pérez-Boada M, Martìnez MJ, Martìnez AT (2001) A new versatile peroxidase from Pleurotus. Biochem Soc Trans 29:116–122
Ruiz-Duenas FJ, Martìnez MJ, Martìnez AT (1999) Heterologous expression of Pleurotus eryngii peroxidase confirms its ability to oxidize Mn2+ and different aromatic substrates. Appl Environ Microbiol 65:4705–4707
Sato T, Hara S, Matsui T, Sazaki G, Saijo S, Ganbe T, Tanaka N, Sugano Y, Shoda M (2004) A unique dye-decolorizing peroxidase, DyP, from Thanatephorus cucumeris Dec 1: heterologous expression, crystallization and preliminary X-ray analysis. Acta Crystallogr D 60:149–152
Shin KS, Oh IK, Kim CJ (1997) Production and purification of Remazol brilliant blue R decolorizing peroxidase from the culture filtrate of Pleurotus ostreatus. Appl Environ Microbiol 63:1744–1748
Soden DM, Dobson AD (2003) The use of amplified flanking region-PCR in the isolation of laccase promoter sequences from the edible fungus Pleurotus sajor-caju. J Appl Microbiol 95553–562
Sugano Y, Ishii Y, Shoda M (2004) Role of H164 in a unique dye-decolorizing heme peroxidase DyP. Biochem Biophys Res Commun 322:126–132
Sugano Y, Nakano R, Sasaki K, Shoda M (2000) Efficient heterologous expression in Aspergillus oryzae of a unique dye-decolorizing peroxidase, DyP, of Geotrichum candidum Dec 1. Appl Environ Microbiol 66:1754–1758
Sugano Y, Sasaki K, Shoda M (1999) cDNA cloning and genetic analysis of a novel decolorizing enzyme, peroxidase gene dyp from Geotrichum candidum Dec 1. J Biosci Bioeng 87:411–417
Vyas BR, Molitoris HP (1995) Involvement of an extracellular H2O2-dependent ligninolytic activity of the white rot fungus Pleurotus ostreatus in the decolorization of Remazol brilliant blue R. Appl Environ Microbiol 61:3919–3927
Wariishi H, Valli K, Gold MH (1992) Manganese(II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium. Kinetic mechanism and role of chelators. J Biol Chem 267:23688–23695
Welinder KG (1992) Superfamily of plant, fungal, and bacterial peroxidases. Curr Opin Struct Biol 2:388–393
Acknowledgements
This work was supported by grants from the Ministero dell’Università e della Ricerca Scientifica (Progetti di Rilevante Interesse Nazionale, PRIN 2004) and from EC FP6 Project SOPHIED (NMP2-CT-2004-505899).
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Faraco, V., Piscitelli, A., Sannia, G. et al. Identification of a new member of the dye-decolorizing peroxidase family from Pleurotus ostreatus . World J Microbiol Biotechnol 23, 889–893 (2007). https://doi.org/10.1007/s11274-006-9303-5
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DOI: https://doi.org/10.1007/s11274-006-9303-5