Abstract
This study reports the identification and demonstration of an organism with antimicrobial activity isolated from activated biomass of an effluent treatment plant (ETP) treating wastewater containing pesticides. While assessing the heterotrophic diversity of biomass collected from ETP, clear zones were observed on Luria Bertani plates. The bacterial isolate producing the zone as well as the bacterial cells surrounding the zone were isolated and purified by sub-culturing. Both isolates were identified by partial sequencing of the 16S rDNA clone. Presence of antimicrobial activity was demonstrated against various laboratory strains, isolated from different treatment plants and also against waterborne pathogens. The isolate that produced antimicrobial activity was identified as Bacillus subtilis strain HPC248 and the sensitive strain was identified as Bacillus sphaericus strain HPC249.
This is a preview of subscription content, log in via an institution to check access.
References
Bush K (1997) Antimicrobial agents. Curr opin Chem Biol 1: 69–175
Cherif A, Quzari H, Daffonchio D, Cherif H, Ben Salma K, Hassen A, Jaoua S, Boudabous A (2001) Thuricin 7: a novel bacteriocin produced by Bacillus thuringiensis BMG1.7, a new strain isolated from soil. Letters in Applied Microbiolog 32: 243–247
Cladera-Olivera F, Caron GR, Brandelli A (2004) Bacteriocin-like substance production by Bacillus licheniformis strain P40. Lett Appl Microbiol 38: 251
Dubey SK, Padmanabhan P, Purohit HJ, Upadhyay SN (2003) Methanotrophs tracking and their diversity in paddy soil: A molecular approach. Curr Sci 85: 92–95
Jack RW, Tagg JR, Ray B (1995) Bacteriocins of gram-positive bacteria. Microbiol Rev 59: 171–200
Kapley A, Lampel K, Purohit. HJ (2000) Development of Duplex PCR for Salmonella and Vibrio. World J Microbiol Biotechnol 16: 457–58
Navarro L, Zarazaga M, Saenz J, Ruiz-Larrea F, Torres C (2000) Bacteriocin production by lactic acid bacteria isolated from Rioja red wines. J Appl Microbiol 88: 44–51
Nes IF, Johnsborg O (2004) Exploration of antimicrobial potential in LAB by genomics. Curr Opin Biotechnol 15: 100–104
Neumüller AM, Konz D, Marahiel MA (2001) The two-component regulatory system BacRS is associated with bacitracin ‘self-resistance’ of Bacillus licheniformis ATCC 10716. Eur J Biochem 268: 3180–3185
Perez C, Suarez C, Guillermo R, Castro GR (1992) Production of antimicrobials by Bacillus subtilis MIR 15. J Biotechnol 26: 331–336
Tagg JR, McGiven AR (1991) Assay system for babacteriocins. Appl Microbiol 21: 943
Vaseeharan B, Ramasamy P (2003) Control of pathogenic Vibrio spp.by Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon. Lett Appl Microbiol 36: 83
Zuber P (2001) A peptide profile of the Bacillus subtilis genome. Peptides 22: 1555–1577
Acknowledgements
This study was supported by a grant from CSIR, New Delhi, India under the network project SMM0002.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kapley, A., Siddiqui, S.N. & Purohit, H.J. A Bacillus subtilis strain HPC248 from an effluent treatment plant with antimicrobial activity. World J Microbiol Biotechnol 23, 879–882 (2007). https://doi.org/10.1007/s11274-006-9296-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11274-006-9296-0