d-Psicose production from d-fructose using an isolated strain, Sinorhizobium sp.
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Sinorhizobium sp., which can convert d-fructose into d-psicose, was isolated from soil. The optimal pH, temperature, and cell concentration for d-psicose production with the isolated strain were 8.5, 40°C, and 60 mg/ml, respectively. The toluene-treated cells showed 2.5- and 4.8-fold increases in the d-psicose concentration and productivity compared with untreated washed cells. Under the optimal conditions, the toluene-treated cells produced 37 g d-psicose/l from 70% (w/v) (3.9 M) d-fructose after 15 h.
KeywordsBiotransformation d-Fructose Isolated strain d-Psicose Sinorhizobium sp.
This study was supported by grant number R01-2004-000-10012-0 from the Basic Research Program of the Korea Science & Engineering Foundation (KOSEF).
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