Skip to main content
SpringerLink
Log in

Decolourisation and dephenolisation potential of selected Aspergillus section Nigri strains – Aspergillus tubingensis in olive mill wastewater

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Aspergillus section Nigri strains Aspergillus aculeatus Ege-K 258, A.foeditus var. pallidus Ege-K156, A. niger Ege-K 4 and A. tubingensis Ege-K 265 were used to treat olive mill wastewater (OMW) in an investigation aimed at exploring their dephenolisation and decolourisation ability and, consequently, the economic feasibility of using any or all of these strains in a pre-treatment step in the processing of OMW. Of these strains A. tubingensis Ege-K 265 resulted in an 80% decolourisation of twofold-diluted OMW and a 30% decolourisation of undiluted OMW; in addition, it was able to remove approximately 30% of all phenolic compounds in both twofold-diluted and undiluted OMW. We conclude that A. tubingensis Ege-K 265 could be effectively used in the pre-treatment step of a combined aerobic-anaerobic process to solve the environmental problems caused by OMW in Mediterranean countries.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Anonymous (1990) Official Methods of Analysis of the Association of Official Analytical Chemists (AOAC), 15th edn. AOAC, Washington D.C. USA

  • Aissam H, Errachidi F, Penninckx MJ, Merzouki M, Benlemlih M (2005) Production of tannase by Aspergillus niger HA37 growing on tannic acid and olive mill waste water. World J Microbiol Biotechnol 21:609–614

    Article  CAS  Google Scholar 

  • Assas N, Ayed L, Marouani L, Hamdi M (2002) Decolourisation of fresh and stored-black olive mill wastewaters by Geotrichum candidum. Process Biochem 38:361–365

    Article  CAS  Google Scholar 

  • Azbar N, Bayram A, Filibeli A, Muezzinoglu A, Sengul F, Ozer A (2004) A review of waste management options in olive oil production. Crit Rev Environ Sci Technol 34: 209–247

    Article  CAS  Google Scholar 

  • Baldrian P (2005) Fungal laccases occurrence and properties. FEMS Microbiol Rev 30:215–242

    Article  CAS  Google Scholar 

  • Blánquez P, Caminal G, Sarra M, Vicent MT, Gabarrell X (2002) Olive mill waste waters decolorization and detoxification in a bioreactor by the white rot fungus Phanerochaete flavido-alba. Biotechnol Progr 18:660–662

    Article  CAS  Google Scholar 

  • Box JD (1983) Investigation of the Folin-Ciocalteau phenol reagent for the determination of polyphenolic substances in natural waters. Water Res 17:511–525

    Article  CAS  Google Scholar 

  • Carlos-Ruiz J, Rubai T, Pérez J, Martinez-Lopez J (2002) Effect of olive mill wastewater on extracellular ligninolytic enzyme produced by Phanerochaete flavido-alba. FEMS Microb Lett 212:41–45

    Article  Google Scholar 

  • D’Annibale A, Crestini C, Vinciguerrra V, Sermanni G (1998) The biodegradation of recalcitrant effluents from an olive mill by white-rot fungus. J Biotechnol 61:209–218

    Article  CAS  Google Scholar 

  • D’Annibale A, Ricci M, Quaratino D, Federici F, Fenice M (2004) Panus tigrinus efficiently removes phenols, colour and organic load from olive-mill wastewater. Res Microbiol 155:596–603

    Article  CAS  Google Scholar 

  • Dias A, Bezerra MR, Pereira AN (2004) Activity and elution profile of laccase during biological decolorization and dephenolization of olive mill wastewater. Bioresource Technol 92:7–13

    Article  CAS  Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith P (1956) Colourimetric method for determination of sugar and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  • Fadil A, Chahlaoui A, Ouahbi A, Zaid A, Borja R (2003) Aerobic biodegradation and detoxification of wastewaters from the olive oil industry. Int Biodeterior Biodegr 51:37–41

    Article  CAS  Google Scholar 

  • Fenice M, Giovannozzi-Sermanni G, Federici F, D’annibale A (2003) Submerged and solid-state production of laccase and Mn-peroxidase by Panus tigrinus on olive mill wastewater-based media. J Biotechnol 100:77–85

    Article  CAS  Google Scholar 

  • Flouri F, Sotrichos D, Ioannidou S, Balis C (1996) Decolorization of olive oil mill liquid wastes by chemical and biological means. Int Biodeterior Biodegradation 38:189–192

    Article  CAS  Google Scholar 

  • Garcia IG, Peňa Jiménez PR, Venceslada JLB, Martin AM, Santos MAM, Gómez ER (2000) Removal of phenol compounds from olive mill wastewater using Phanerochate chrysosporium, aspergillus niger, Aspergillus terreus and Geotrichum candidum. Process Biochem 35:751–758

    Article  CAS  Google Scholar 

  • Hamdi M, Khadir A, Garcia JL (1991) The use of Aspergillus niger for the bioconversion of olive mill waste-waters. Appl Microbiol Biotechnol 34:828–831

    Article  CAS  Google Scholar 

  • Jaouani A, Sayadi S, Vanthournhout M, Penninckx MJ (2003) Potent fungi for decolourisation of olive oil mill wastewaters. Enzyme Microb Technol 33:802–809

    Article  CAS  Google Scholar 

  • Kissi M, Mountadar M, Assobhei O, Gargiulo E,Palmieri G, Giardina P, Sannia G (2001) Roles of two white-rot basidiomycete fungi in decolorisation and detoxification of olive mill waste water. Appl Microbiol Biotechnol 57:221–226

    Article  CAS  Google Scholar 

  • Martirani L, Giardina P, Marzullo L, Sannia G (1996) Reduction of phenol content and toxicity in olive oil mill wastewaters with the ligninolytic fungus Pleurotus ostreatus. Water Res 30:1914–1918

    Article  CAS  Google Scholar 

  • Mondal KC, Banerjee D, Jana M, Pati BR (2001) Colourimetric assay method for determination of the tannin acyl hydrolase (E.C. 3.1.1.20) activity. Anal Biochem 295:168–171

    Article  CAS  Google Scholar 

  • Nieto L Hoyos SE, Rubio F, Pareja MP, Cormenzana A (1993) The biological purification of waste products from olive oil extraction. Bioresource Technol 43:215–219

    Article  Google Scholar 

  • Paszczyński A, Crawford RL, Huynh VB (1988) Manganese peroxidase of Phanerochaete chrysosporium: Purification. Methods Enzymol 161:264–270

    Article  Google Scholar 

  • Palmieri G, Giardinai P, Bianco C, Scaloni A, Capasso A, Sannia GA (1997) Novel white laccase from Pleuroutus ostreatus. J Biol Chem 272:31301–31307

    Article  CAS  Google Scholar 

  • Perez J, Rubai T, Ben-Hamman O, Martinez J (1998) Phanerochaete flavido-alba laccase induction and modification of manganese peroxidase isoenzyme pattern in decolorized olive oil mill wastewaters. Appl Environ Microbiol 64:2726–2729

    CAS  Google Scholar 

  • Pinto GAS, Leite SGF, Terzi SC, Couri S (2001) Selection of tannase-producing Aspergillus niger strains. Braz J Microbiol 32:24–26

    CAS  Google Scholar 

  • Sayadi S, Ellouz R (1993) Screening of white rot fungi for the treatment of olive mill waste-waters. J Chem Technol Biotechnol 57:141–146

    Article  CAS  Google Scholar 

  • Sayadi S, Ellouz R (1995) Roles of lignin peroxidase and manganese peroxidase from Phanerochaete chrysosporium in the decolorization of olive mill wastewaters. Appl Environ Microbiol 61:1098–1103

    CAS  Google Scholar 

  • Scherer M, Fischer R (2001) Molecular characterization of a blue-copper laccase, TILA, of Aspergillus nidulans. FEMS Microbiol Lett 199:207–213

    Article  CAS  Google Scholar 

  • Tsioulpas A, Dimou D, Iconomou D, Aggelis G (2002) Phenolic removal in olive mill wastewater by strains of Pleurotus spp. in respect to their phenol oxidase (laccase) activity. Bioresource Technol 84:251–257

    Article  CAS  Google Scholar 

  • Varga J, Rigo K, Toth B, Teren J, Kozakiewicz Z (2003) Evolutionary relationship among Aspergillus strains producing economically important mycotoxins. Food Technol Biotechnol 41:29–36

    CAS  Google Scholar 

  • Vinciguera V, D’Annibale A, Monache D, Sermani GG (1995) Correlated effects during the bioconversion of waste olive waters by Lentinus edodes. Bioresource Technol 51:221–226

    Article  Google Scholar 

  • Yeşilada O, Sik S, Sam M (1998) Biodegradation of olive mill waste water by Coriolus versicolour and Funalia trogii: Effects of agitation, initial COD concentration, inoculum size and immobilization. World J Microbiol Biotechnol 14:37–42

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bioengineering Department, Ege University, Faculty of Engineering, 35100, Bornova, Izmir, Turkey

    Gaye Öngen, Gaye Güngör & Bahar Kanberoglu

Authors
  1. Gaye Öngen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gaye Güngör
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bahar Kanberoglu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gaye Öngen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Öngen, G., Güngör, G. & Kanberoglu, B. Decolourisation and dephenolisation potential of selected Aspergillus section Nigri strains – Aspergillus tubingensis in olive mill wastewater. World J Microbiol Biotechnol 23, 519–524 (2007). https://doi.org/10.1007/s11274-006-9254-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-006-9254-x

Keywords

Search

Navigation