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Production of thermostable and neutral glucoamylase using immobilized Thermomucor indicae-seudaticae

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Abstract

Thermomucor indicae-seudaticae was immobilized in alginate, κ-carrageenan, agarose, agar, polyacrylamide and loofah (Luffa cylindrica) sponge (as such or coated with alginate/starch/Emerson YpSs agar), and used for the production of glucoamylase in submerged fermentation. The mycelium developed from alginate-immobilized sporangiospores secreted higher glucoamylase titres (22.7 U ml−1) than those immobilized in other gel matrices and the freely growing mycelial pellets (18.5 U ml−1). Loofah network provided a good support for mycelial growth, but the enzyme production was lower than that attained with alginate beads. Glucoamylase production increased with inoculum density and the optimum levels were achieved when 40 calcium alginate beads (∼5 × 106 immobilized spores) were used to inoculate 50 ml production medium. The alginate bead inoculum displayed high storage stability at 4°C and produced comparable enzyme titres up to 120 days. The glucoamylase production by hyphae emerged from the immobilized sporangiospores was almost stable over eight batches of repeated fermentation. Scanning electron micrographs of alginate beads, after batch fermentation, revealed extensive mycelial growth inside and around the beads.

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Acknowledgements

We wish to thank University Grants Commission, Government of India for providing financial assistance during the course of this investigation, and Mr. Rajesh Pathania and Mr. Girish (All India Institute of Medical Sciences, New Delhi, India) for providing technical assistance in carrying out scanning electron microscopy of the alginate beads. Pardeep Kumar is also grateful to Council of Scientific and Industrial Research, Government of India for awarding Junior/Senior Research Fellowship.

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  1. Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110 021, India

    Pardeep Kumar & T. Satyanarayana

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  1. Pardeep Kumar
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  2. T. Satyanarayana
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Correspondence to T. Satyanarayana.

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Kumar, P., Satyanarayana, T. Production of thermostable and neutral glucoamylase using immobilized Thermomucor indicae-seudaticae . World J Microbiol Biotechnol 23, 509–517 (2007). https://doi.org/10.1007/s11274-006-9253-y

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  • DOI: https://doi.org/10.1007/s11274-006-9253-y

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