Abstract
A novel raw-starch-digesting glucoamylase producer, Rhizopus sp. W-08, and Saccharomyces cerevisiae Z-06 were used in a fed batch process for simultaneous saccharification and fermentation of raw corn flour. Ethanol concentration of 21% (v/v) was obtained after 48 h. The conversion efficiency of raw corn flour to ethanol was 94.5% of the theoretical ethanol yield.
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Abu EA, Ado SA, James B (2005) Raw starch degrading amylase production by mixed culture of Aspergillus niger and Saccharomyces cerevisae grown on sorghum pomace. Afr J Biotechnol 4:785–790
Atiyeh H, Duvnjak Z (2003) Production of fructose and ethanol from cane molasses using Saccharomyces cerevisiae ATCC 36858. Acta Biotechnol 23:37–48
Dai FL (1987) Characteristics and identification of fungi ISBN 7-5019-1292-0. Science Press, Beijing, pp 57–64
Ge XY, Zhang WG (2005) A shortcut to the production of high ethanol concentration from Jerusalem Artichoke tubers. Food Technol Biotechnol 43:241–246
Goyal N, Gupta JK, Soni SK (2005) A novel raw starch digesting thermostable a-amylase from Bacillus sp. I-3 and its use in the direct hydrolysis of raw potato starch. Enzyme Microbl Technol 37:723–734
Hayashida S, Ohta K (1980) Effects of phosphatidylcholine or ergosteryl oleate on physiological properties of Saccharomyces sake. Agr Biol Chem 44:2561–2567
Hayashida S, Ohta K (1981) Formation of high concentration of alcohol by various yeasts. J Inst Brewing 87:42–44
Iqbal Z, Rajoka MH, Jabbarr A, Malana MA, Khalid AM, Rajoka MI (2003) Kinetics of enhance thermostability of extracellular glucoamylase from Arachniotus sp. Biotechnol Lett 25:1667–1670
Katarzyna S, Jacek N, Zbigniew C (2004) Use of Zymomonas mobilis and Saccharomyces cerevisiae mixed with Kluyveromyces fragilis for improved ethanol production from Jerusalem artichoke tubers. Biotechnol Lett 26:845–848
Lesage L, Genot C, Record E, Pouliquen C, Richard-Molard D (1993) Fatty acid composition and molecular order of phospolipids from Eurotium chevalieri in response to change in water activity. J Gen Microbiol 139:1653–1661
Maldonado MC, Strasser de Saad AM (1998) Production of pectinesterase and polygalacturonase by Aspergillus niger in submerged and solid state systems. J Indust Microbiol Biotechnol 20:34–38
Margaritis A, Merchant FJ, Veliky IA (1982) High concentration-ethanol fermentation of raw ground corn. Agri Biol Chem 46:1947–1950
Mitsuiki S, Mukae K, Sakai M, Goto M, Hayashida S, Furukawa K (2005) Comparative characterization of raw starch hydrolyzing a-amylase from various Bacillus strains. Enzyme Microb Technol 37:410–416
Nakamura T, Ogata Y, Hamada S, Ohta K (1996) Ethanol production from Jerusalem artichoke tubers by Aspergillus niger and Saccharomyces cerevisiae. J Ferment Bioeng 81:564–566
Rajoka M, Yasmeen A (2005) Induction, and production studies of a novel glucoamylase of Aspergillus niger. World J Microbiol Biotechnol 21:179–187
Turner PC, Mclennan AG, Bates AD, White MRH (2002) Regulation of transcription in prokaryotes. Instant notes in molecular biology, ISBN 7-03-007305-3. BIOS Scientific Publishers Limited, UK, pp 179–182
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Wang, LS., Ge, XY. & Zhang, WG. Improvement of ethanol yield from raw corn flour by Rhizopus sp.. World J Microbiol Biotechnol 23, 461–465 (2007). https://doi.org/10.1007/s11274-006-9247-9
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DOI: https://doi.org/10.1007/s11274-006-9247-9