Abstract
The inadequate supply of oxygen to biomass is a critical factor to the productivity of most aerobic submerged fermentations. This happens because oxygen is sparingly soluble in the aqueous media. The use of a second liquid phase of perfluorocarbon (PFC), an oxygen-carrying compound, in the culture medium can increase the availability of oxygen to the microorganisms. The effect of perfluorodecalin on Yarrowia lipolytica cultures was investigated in shake-flask cultures. It was found that the specific growth rate of Y. lipolytica, a strictly aerobic yeast, increases with increasing PFC concentration. Extracellular lipase production was increased with 20% (v/v) of PFC and agitation of 250 rev/min. It was shown that the PFC presence benefitted lipase production and not just its secretion to the extracellular medium.
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The authors thank financial support from CNPq and FAPERJ (Brazil), FCT and CICECO (Portugal), and GRICES/CAPES (102/03).
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Amaral, P.F.F., de Almeida, .P.R., Peixoto, T. et al. Beneficial effects of enhanced aeration using perfluorodecalin in Yarrowia lipolytica cultures for lipase production. World J Microbiol Biotechnol 23, 339–344 (2007). https://doi.org/10.1007/s11274-006-9229-y
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DOI: https://doi.org/10.1007/s11274-006-9229-y