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Molecular sequencing and analysis of soluble methane monooxygenase gene clusters from methanotroph Methylomonas sp. GYJ3

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Summary

A methanotroph Methylomonas sp. GYJ3 was isolated, whose sMMO genes and 16S rDNA were sequenced and analysed, demonstrating that the bacterium might be a type I methanotroph (γ-Proteobacteria) and was closer to Methylomonas sp. KSWIII/KSPIII. This result was consistent with the result previously determined by biochemistry and morphological taxonomy. Sequence comparison among six open reading frames and the deduced amino acid sequences of the sMMO genes from six strains revealed that the strain GYJ3 had highly conserved regions in MMOX with other strains, amounting to 78–99% homology at protein level and 71–97% homology at DNA level. Highly conserved sequences lay in two iron-binding regions. Furthermore, scanning electron microscopy of the strain GYJ3 showed rod shapes with a slightly bent configuration on the even surfaces and with plump bodies.

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Abbreviations

MMO:

methane monooxygenase

sMMO:

soluble MMO

pMMO:

particulate MMO

MMOH:

hydroxylase

MMOX:

α subunit of MMOH

MMOY:

β subunit of MMOH

MMOZ:

γ subunit of MMOH

ORFY/MMOD:

an unknown function component of MMO

MMOB:

a coupling protein of MMO

MMOC:

reductase of MMO

SEM:

scanning electron microscopy

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Acknowledgments

This work was supported by National Natural Science Foundation of China (No.20573124).

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Authors and Affiliations

  1. State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, P.R. China

    Shao-feng Hua & Shu-ben Li

  2. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, P.R. China

    Hai-dong Tan

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  1. Shao-feng Hua
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  2. Shu-ben Li
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Correspondence to Shu-ben Li.

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Hua, Sf., Li, Sb. & Tan, Hd. Molecular sequencing and analysis of soluble methane monooxygenase gene clusters from methanotroph Methylomonas sp. GYJ3 . World J Microbiol Biotechnol 23, 323–330 (2007). https://doi.org/10.1007/s11274-006-9227-0

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  • DOI: https://doi.org/10.1007/s11274-006-9227-0

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