Abstract
Laccase is a ligninolytic enzyme that is widespread in white-rot fungi. Alginate–chitosan microcapsules prepared by an emulsification–internal gelation technique were used to immobilize laccase. Parameters of the immobilization process were optimized. Under the optimal immobilization conditions (2% sodium alginate, 2% CaCl2, 0.3% chitosan and 1:8 ratio by volume of enzyme to alginate), the loading efficiency and immobilized yield of immobilized laccase were 88.12% and 46.93%, respectively. Laccase stability was increased after immobilization. Both the free and immobilized laccase alone showed a very low decolorization efficiency when Alizarin Red was selected for dye decolorization test. When 0.1 mM 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was added into the decolorization system, the decolorization efficiency increased significantly. Immobilized laccase retained 35.73% activity after three reaction cycles. The result demonstrated that immobilized laccase has potential application in dyestuff treatment.
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Acknowledgements
The authors want to thank Novozymes for providing the laccase preparations. They would also like to thank Li Zhang from the Electron Microscopic Center, Harbin Medical University, Harbin, China for her assistance for collecting the SEM images.
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Lu, L., Zhao, M. & Wang, Y. Immobilization of Laccase by Alginate–Chitosan Microcapsules and its Use in Dye Decolorization. World J Microbiol Biotechnol 23, 159–166 (2007). https://doi.org/10.1007/s11274-006-9205-6
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DOI: https://doi.org/10.1007/s11274-006-9205-6