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New insight in staphylococcin research: bacteriocin and/or bacteriocin-like inhibitory substance(s) produced by S. aureus AB188

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Summary

Staphylococcus aureus AB188 (a clinical isolate from wound pus) has been found to produce bacteriocins and/or bacteriocin-like inhibitory substance(s) tentatively termed staphylococcin Bac188. It has a broad activity spectrum against many Gram-positive (e.g. B. subtilis, S. aureus, E. faecalis), Gram-negative bacteria (e.g. E. coli, S. typhi and S. dysenteriae), and dermatophytes including Microsporum  canis, Microsporum  gypseum, Trichophyton mentagrophytes, Trichophyton  longi and Trichophyton  rubrum. Interestingly, staphylococcin Bac188 also showed very potent activity against many clinical isolates of Mycobacterium tuberculosis. Staphylococcin Bac188 showed wide thermostability and remained stable over the wide pH range (pH 2–14). It was also resistant to treatment with chloroform, catalase, lipase and lysozyme, but showed sensitivity to proteinase K, trypsin and α-chymotrypsin suggesting its proteinaceous nature. Staphylococcin Bac188 revealed bactericidal effects on the S. aureus SS-1 sensitive strain as well as on E. coli and S. typhi, suggesting a similar mode of action on both Gram-negative and Gram-positive organisms. The antimicrobial, antidermatophytic and antimycobacterial activities expressed by S. aureus AB188 correlate with the production of a bacteriocin and/or bacteriocin-like inhibitory substance with properties similar to other staphylococcins reported earlier. To our knowledge, this is the first report describing such wide possible clinical applications of a bacteriocin and/or bacteriocin-like inhibitory substance produced by S. aureus AB188, suggesting further investigation for potential therapeutic development.

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Acknowledgments

This project is partly supported by the Higher Education Commission of Pakistan (HEC-No. 20–151/Acad-R/03) and the Pakistan Science Foundation (PSF-S-KU/CHEM-363) to S.A. Ali and S.A. Rasool.

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Author notes

  1. Syed Abid Ali

    Present address: Present address: Interfakultäres Institut für Biochemie, Universität Tübingen, Hoppe-Seyler-Straß 4, D-72076, Tübingen, Germany

Authors and Affiliations

  1. Laboratory of Molecular Genetics, Department of Microbiology, University of Karachi, 75270, Karachi, Pakistan

    Sadia Saeed, Sheikh Ajaz Rasool & Samia Ahmed

  2. Jinnah University for Women, 75270, Karachi, Pakistan

    Tanweer Khanum

  3. International Center for Chemical Sciences, HEJ Research Institute of Chemistry, University of Karachi, 75270, Karachi, Pakistan

    Muhammad Bashir Khan, Atiya Abbasi & Syed Abid Ali

Authors
  1. Sadia Saeed
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  2. Sheikh Ajaz Rasool
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  3. Samia Ahmed
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  4. Tanweer Khanum
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  5. Muhammad Bashir Khan
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  6. Atiya Abbasi
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  7. Syed Abid Ali
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Correspondence to Syed Abid Ali.

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Saeed, S., Rasool, S.A., Ahmed, S. et al. New insight in staphylococcin research: bacteriocin and/or bacteriocin-like inhibitory substance(s) produced by S. aureus AB188. World J Microbiol Biotechnol 22, 713–722 (2006). https://doi.org/10.1007/s11274-005-9095-z

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  • DOI: https://doi.org/10.1007/s11274-005-9095-z

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