Summary
Pseudomonas fluorescens MSP-393, a plant growth-promoting rhizobacterium is an efficient biocontrol agent in rice grown in saline soils of coastal ecosystems. To understand the mechanism of salt tolerance, proteome analysis of the bacterium was carried out employing two-dimensional gel electrophoresis and MALDI-TOF. This technique was used to investigate the regulation of gene product expression of P. fluorescens MSP-393 grown under high osmolarity and used peptide mass fingerprinting and in silico investigation to identify those proteins with altered expression. Among them 15 were assigned to proteins with known functions. Their roles in response to salt stress are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anderson M.S., Raetz C.R.H., 1987 Biosynthesis of lipid A precursors in Escherichia coli, a cytoplasmic acyltransferase that converts UDP-N-acetylglucosamine to UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine Journal of Biological Chemistry 262:5159–5169
Buckel S.D., Bell A.W., Mohana Rao J.K., Hermodson M.A. 1986 An analysis of the structure of the product of the rbsA gene of Escherichia coli K12 Journal of Biological Chemistry 261:7659–7662
Crosa J.H., 1997 Signal transduction and transcriptional and post-transcriptional control of iron-regulated genes in bacteria Microbiology and Molecular Biology Reviews 61:319–336
da Costa M.S., Santos H., Galinski E.A., 1998 An overview of the role and diversity of compatible solutes in Bacteria and Archaea Advances in Biochemical Engineering/Biotechnology 61:117–53
Defago G., Berling C.H., Burger U., Haas D., Kahr G., Keel C., Voisard C., Wirthner P., Wurthrich B. 1990 Suppression of black root rot of tobacco and other root diseases by strains of Pseudomonas fluorescens: potential applications and mechanisms In Hornby D., (ed) Biological Control of Soil-Borne Pathogens CAB International Wallingford, UK. pp. 93–108 ISBN 0851986374
Fenton W.A., Horwich A.L., 1997 GroEL-mediated protein folding Protein Science 6:743–760
Glaasker E., Heuberger E.H.M.L., Konings W.N., Poolman B., 1998 Mechanism of osmotic activation of the quaternary ammonium compound transporter (Qac T) of L. plantarumJournal of Bacteriology 180:5540–5546
Hartl F.U., Hlodan R., Langer T., 1994 Molecular chaperones in protein folding: the art of avoiding sticky situations Trends in Biochemical Sciences 19:20–25
Joanna C.W., Karen A., Homer, Beighton D., 2001 Altered protein expression of Streptococcus oralis cultured at low pH revealed by two-dimensional gel electrophoresis Applied and Environmental Microbiology 67:3396–3405
Jonathan E.V., Jeffrey K.I., Clarke S., 1998 Mutations in the Escherichia coli surE gene increase isoaspartyl accumulation in a strain lacking the pcm repair methyltransferase but suppress stress-survival phenotypes FEMS Microbiology Letters 167:19–25
Julianne L., Thomas T., Cavicchioli R., 2000 Low temperature regulated DEAD-box RNA helicase from the antarctic archaeon, Methanococcoides burtonii Journal of Molecular Biology 297:553–567
Kisseleva L.L., Justesenc J., Alexey D., Wolfson Z.,Yu L. Frolovaa, 1998 Diadenosine oligophosphates (ApnA), a novel class of signalling molecules? FEBS Letters 427:157–163
Kloepper J.W., Schroth M.N., Miller T.D., 1980 Effect of rhizosphere colonization by plant growth promoting rhizobacteria on potato development and yield Phytopathology 70:1078–1082
Laemmli U.K., 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227:680–685
Narendra, T. 2005 Unwinding after high salinity stress: development of salinity tolerant plant without affecting yield. ISB News Report, March 2005
O’Farrell P.H., 1975 High resolution two-dimensional electrophoresis of proteins Journal of Biological Chemistry 250:4007–4021
Rangarajan S., Saleena L.M., Vasudevan P., Nair S., 2003 Biological suppression of rice diseases by Pseudomonas spp. under saline soil conditions Plant and Soil 251:73–82
Robinson, P.M. 1999 The enzymology of osmoregulation in archaea: glutamine synthetase and β-glutamine production. PhD Thesis Boston College DAI-B 60/06 p. 2677
Robinson P., Neelon K., Schreier H.J., Roberts M.F., 2001 β-Glutamate as a substrate for glutamine synthetase Applied and Environmental Microbiology 67:4458–4463
Schmalisch M., Langbein I., Stulke J., 2002 The general stress protein Ctc of Bacillus subtilis is a ribosomal protein Journal of Molecular Microbiology and Biotechnology 4:495–501
Shen S.H., Matsubae M., Takao T., 2002 A proteomic analysis of leaf sheaths from rice Journal of Biochemistry 132:613–620
Tohru M., Chu R., Yamaguchi S., Macnab R.M., 2000 Role of FliJ in flagellar protein export in Salmonella Journal of Bacteriology 182:4207–4215.
Volker U., Engelmann S., Maul B., Riethdorf S., Volker A., Schmid R., Mach H., Hecker M., 1994 Analysis of the induction of general stress proteins of Bacillus subtilis Microbiology 140:741–752
Walt A., Kahn M.L., 2002 The fixA and fixB gene are necessary for anaerobic carnitine reduction in Escherichia coli Journal of Bacteriology 184:4044–4047
Yale J., Bohnert H.J., 2001 Transcript expression in Saccharomyces cerevisiae at high salinity Journal of Biological Chemistry 276:15996–16007
Acknowledgements
The financial aid from the Department of Biotechnology, Government of India and MALDI-TOF facility from Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India are gratefully acknowledged.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Paul, D., Dineshkumar, N. & Nair, S. Proteomics of a plant growth-promoting rhizobacterium, Pseudomonas fluorescens MSP-393, subjected to salt shock. World J Microbiol Biotechnol 22, 369–374 (2006). https://doi.org/10.1007/s11274-005-9043-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11274-005-9043-y