Summary
One hundred and thirty endophytic fungi isolated from 12 Chinese traditional medicinal plants collected at Yuanmou county and Dawei Mountain, Yunnan province, southwest China, were tested for antitumour and antifungal activities by MTT assay on human gastric tumour cell line BGC-823 and the growth inhibition test against 7 phytopathogenic fungi. The results showed that fermentation broths from 9.2% of the isolates exhibited antitumour activity and 30% exhibited antifungal activity, moreover, some of them exhibited broad-spectrum antifungal activity. The active isolates were identified to 32 taxa. The results indicate that the endophytic fungi of Chinese traditional medicinal plants are promising sources of novel bioactive compounds.
Similar content being viewed by others
References
M.C. Alley D.A. Scudiero A. Monks M.L. Hursey M.J. Czerwinski D.L. Fine (1988) ArticleTitleFeasibility of drug screening with panels of human tumour cell lines using a microculture tetrazolium assay Cancer Research 48 589–601 Occurrence Handle1:STN:280:DyaL1c%2FotVSrtg%3D%3D
A.E. Arnold Z. Maynard G.S. Gilbert P.D. Coley T.A. Kursat (2000) ArticleTitleAre tropical fungal endophytes hyperdiverse? Ecology Letters 3 267–274 Occurrence Handle10.1046/j.1461-0248.2000.00159.x
H.L. Barnett B.B. Hunter (1987) Illustrated Genera of Imperfect Fungi Macmillan Publishing Company. New York ISBN0-02-306395-5
G.F. Bills J.D. Polishook (1991) ArticleTitleMicrofungi from Carpinus caroliniana Canadian Journal of Botany 69 1477–1482 Occurrence Handle10.1139/b91-191
G. Carroll (1988) ArticleTitleFungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont Ecology 69 2–9
K. Clay (1990) ArticleTitleFungal endophytes of grass Annual Review of Ecology Systems 21 275–297
K. Clay J. Holah (1999) ArticleTitleFungal endophyte symbiosis and plant diversity in successional fields Science 285 1742–1744 Occurrence Handle10.1126/science.285.5434.1742 Occurrence Handle1:CAS:528:DyaK1MXlvFKht7Y%3D
P.J. Fisher A.E. Anson O. Petrini (1984) ArticleTitleAntibiotic activity of some endophytic fungi from ericaceous plants Botanica Helvetica 94 249–253
Y.J. Huang J.F. Wang G.L. Li Z.H. Zheng W.J. Su (2001) ArticleTitleAntitumour and antifungal activities in endophytic fungi isolated from pharmaceutical plants Taxus mairei, Cephalataxus fortunei and Torreya grandis FEMS Immunology and Medical Microbiology 31 163–167 Occurrence Handle1:CAS:528:DC%2BD3MXmsFemtbs%3D
J.Y. Li J.K. Harper D.M. Grant B.O. Tombe B. Bashyal W.M. Hess G.A. Strobel (2001) ArticleTitleAmbuic acid, a highly functionalized cyclohexenone with antifungal activity from Pestalotiopsis spp. And Monochaetia sp Phytochemistry 56 463–468 Occurrence Handle10.1016/S0031-9422(00)00408-8 Occurrence Handle1:CAS:528:DC%2BD3MXhslylt70%3D
C.H. Liu W.X. Zou H. Lu R.X. Tan (2001) ArticleTitleAntifungal activity of Artemisia annua endophyte cultures against phytopathogenic fungi Journal of Biotechnology 88 277–282 Occurrence Handle10.1016/S0168-1656(01)00285-1 Occurrence Handle1:CAS:528:DC%2BD3MXkvVenur8%3D
H. Lu W.X. Zou J.C. Meng J. Hu R.X. Tan (2000) ArticleTitleNew bioactive metabolites produced by Colletotrichum sp., an endophytic fungus in Artemisia annua Plant Science 151 67–73 Occurrence Handle1:CAS:528:DyaK1MXotVKqtbo%3D
T. Mosmman (1983) ArticleTitleRapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays Journal of Immunological Methods 65 55–63
M. Omacini E.J. Chaneton C.M. Ghersa C.B. Müller (2001) ArticleTitleSymbiotic fungal endophytes control insect host-parasite interaction webs Nature 409 78–81 Occurrence Handle10.1038/35051070 Occurrence Handle1:CAS:528:DC%2BD3MXkt1WqtA%3D%3D
J.-H. Park J.H. Park G.J. Choi S.W. Lee K.S. Jang Y.H. Choi K.Y. Cho J.C. Kim (2003) ArticleTitleScreening for Antifungal Endophytic Fungi Against Six Plant Pathogenic Fungi Mycobiology 31 179–182
B Schutz (2001) Endophytic fungi: a source of novel biologically active secondary metabolites. British Mycological Society, International Symposium Proceedings. University of Wales Swansea
A. Stierle G. Strobel D. Stierle (1993) ArticleTitleTaxol and taxane production by Taxomyces andreanae, an endophytic fungus of pacific yew (Taxus brevifolia) Science 260 214–216 Occurrence Handle1:CAS:528:DyaK3sXit12qt74%3D
J.K. Stone C.W. Bacon J.F. White SuffixJr. (2000) An overview of endophytic microbes: endophytism defined C.W. Bacon J.F. White SuffixJr. (Eds) Microbial Endophytes Marcel Dekker New York 3–29 ISBN0-8247-8831-1
G. Strobel B. Daisy (2003) ArticleTitleBioprospecting for microbial endophytes and their natural products Microbiology and Molecular Biology Reviews 67 491–502 Occurrence Handle10.1128/MMBR.67.4.491-502.2003 Occurrence Handle1:CAS:528:DC%2BD2cXmt1SrtA%3D%3D
G.A. Strobel R. Torczynski A. Bollon (1997) ArticleTitleAcremonium sp. a leucinostatin A producing endophyte of European yew (Taxus baccata) Plant Science 128 97–108 Occurrence Handle10.1016/S0168-9452(97)00131-3 Occurrence Handle1:CAS:528:DyaK2sXlslWisbg%3D
G.A. Strobel X.S. Yang J. Sears R. Kramer R.S. Sidhu W.M. Hess (1996) ArticleTitleTaxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallachiana Microbiology 142 435–440 Occurrence Handle1:CAS:528:DyaK28XhtlSitrY%3D Occurrence Handle10.1099/13500872-142-2-435
W. Suthep S. Nongluksna P. Wattana T. Nuntawan D. Kannawat R. Nijsiri M. Vithaya (2004) ArticleTitleEndophytic fungi with anti-microbial, anti-cancer and anti-malarial activities isolated from Thai medicinal plants World Journal of Microbiology and Biotechnology 20 265–272
M. Vurro A. Evidente A. Andolfi M.C. Zonno F. Giordano A. Motta (1998) ArticleTitleBrefeldin A and α,β-dehydrocurvularin, two phytotoxins from Alternaria zinniae, a biocontrol agent of Xanthium occidental Plant Science 138 67–79 Occurrence Handle10.1016/S0168-9452(98)00131-9 Occurrence Handle1:CAS:528:DyaK1cXmt1ektbw%3D
J.F. Wang Y.J. Huang M.J. Fang Y.J. Zhang Z.H. Zheng Y.F. Zhao W.J. Su (2002) ArticleTitleBrefeldin A, a cytotoxin produced by Paecilomyces sp. and Aspergillus clavatus isolated from Taxus mairei and Torreya grandis FEMS Immunology and Medical Microbiology 34 51–57 Occurrence Handle10.1016/S0928-8244(02)00346-2 Occurrence Handle1:CAS:528:DC%2BD38Xms12huro%3D
J.F. Wang G.L. Li H.Y. Lu Z.H. Zheng Y.J. Huang W.J. Su (2000) ArticleTitleTaxol from Tubercularia sp. strain TF5, an endophytic fungus of Taxus mairei FEMS Microbiology Letters 193 249–253 Occurrence Handle10.1016/S0378-1097(00)00491-2 Occurrence Handle1:CAS:528:DC%2BD3cXosFequr4%3D
D. Weber O. Sterner T. Anke S. Gorzalczancy V. Martino C. Acevedo (2004) ArticleTitlePhomol, a New Antiinflammatory Metabolite from an Endophyte of the Medicinal Plant Erythrina crista-galli The Journal of Antibiotics 57 559–563 Occurrence Handle1:CAS:528:DC%2BD2cXotVyls7Y%3D
Y. Xiang J. Liu A.G. Lu J.X. Cui (2003) ArticleTitleStructure identification for compound separated and purified from taxoids-produced endophytic fungi (Alternaria.alternata var.taxi 101) Journal of Forestry Research 14 217–220 Occurrence Handle1:CAS:528:DC%2BD3sXoslOns7g%3D
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li, H., Qing, C., Zhang, Y. et al. Screening for Endophytic Fungi with Antitumour and Antifungal Activities from Chinese Medicinal Plants. World J Microbiol Biotechnol 21, 1515–1519 (2005). https://doi.org/10.1007/s11274-005-7381-4
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11274-005-7381-4