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DNA Fingerprinting of Paecilomyces Strains of Potential use for the Biological Control of Pests

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Summary

Telomeric fingerprinting was found to be highly differentiating for Paecilomyces fumosoroseus and Paecilomyces lilacinus isolates in comparison to intron splice site PCR and is therefore a good method for quality control of future products based on these fungi. Although the telomeric restriction length polymorphisms correctly divided the isolates into their appropriate species, further correlation with host range or geographical origin of the isolates was not found. In this respect, intron splice site PCR was more informative taxonomically. The chromosome numbers inferred from telomeric fingerprints were seven chromosomes for P. lilacinus and between six and nine chromosomes for P. fumosoroseus.

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References

  • M. Barros Lopes Particlede A. Soden P.A. Henschke P. Langridge (1996) ArticleTitlePCR differentiation of commercial yeast strains using intron splice site primers Applied and Environmental Microbiology 62 4514–4520

    Google Scholar 

  • J.K. Brown (1994) ArticleTitleCurrent status of Bemisia tabaci as a plant pest and virus vector in agrosystems worldwide FAO Plant Protection Bulletin 42 3–32

    Google Scholar 

  • F.A. Cantone D. Vandenberg (1998) ArticleTitleIntraspecific diversity in Paecilomyces fumosoroseus Mycological Research 102 209–215 Occurrence Handle10.1017/S0953756297004590

    Article  Google Scholar 

  • R.M.D.G. Carneiro (1992) ArticleTitlePrincípios e tendências de controle biologíco de nematóides com fungos nematófagos Pesquisa Agropecuária Brasileira 27 113–121

    Google Scholar 

  • K.H. Domsch W. Gams T.H. Anderson (1980 ) Compendium of Soil Fungi NumberInSeriesVol. 1 Academic Press London

    Google Scholar 

  • J. Fargues M.C. Bon S. Manguin Y. Couteaudier (2002) ArticleTitleGenetic variability among Paecilomyces fumosoroseus isolates from various geographical and host insect origins based on the rDNA-ITS regions Mycological Research 106 1066–1074 Occurrence Handle10.1017/S0953756202006408 Occurrence Handle1:CAS:528:DC%2BD38XpsVCgurg%3D

    Article  CAS  Google Scholar 

  • T.S. Gunasekera R.J. Holland M.R. Gillings D.A. Briscoe D.C. Neethling K.L. Williams K.M.H. Nevalainen (2000) ArticleTitlePhenotypic and genetic characterization of Paecilomyces lilacinus strains with biocontrol activity against root-knot nematodes Canadian Journal of Microbiology 46 775–783 Occurrence Handle10.1139/cjm-46-9-775 Occurrence Handle1:CAS:528:DC%2BD3cXmvVGlu7w%3D

    Article  CAS  Google Scholar 

  • S.C. Gupta T.D. Leathers D.T. Wicklow (1993) ArticleTitleHydrolytic enzymes secreted by Paecilomyces lilacinus cultured on sclerotia of Aspergillus flavus Applied Microbiology and Biotechnology 39 99–103 Occurrence Handle1:CAS:528:DyaK3sXktVCltro%3D

    CAS  Google Scholar 

  • R.J. Holland K.L. Williams A. Khan (1999) ArticleTitleInfection of Meloidogyne javanica by Paecilomyces lilacinus Nematology 1 131–139 Occurrence Handle10.1163/156854199508090

    Article  Google Scholar 

  • A. Horowitz (1986) ArticleTitlePopulation dynamics of Bemisia tabaci (Gennadius) with special emphasis on cotton fields Agriculture, Ecosystems and Environment 17 37–47

    Google Scholar 

  • J.W. Ijdo R.A. Wells A. Baldini S.T. Reeders (1991) ArticleTitleImproved telomere detection using a telomere repeat probe (TTAGGG)n generated by PCR Nucleic Acids Research 19 4780 Occurrence Handle1:CAS:528:DyaK3MXmtVSgsL8%3D

    CAS  Google Scholar 

  • P.W. Inglis B.P. Magalães M.C. Valadares-Inglis (1999) ArticleTitleCharacterisation of the entomopathogenic fungus, Metarhizium flavoviride using telomeric fingerprinting FEMS Microbiology Letters 179 49–52 Occurrence Handle10.1016/S0378-1097(99)00390-0 Occurrence Handle1:CAS:528:DyaK1MXlvVWlu7c%3D

    Article  CAS  Google Scholar 

  • L.A. Lacey A.A. Kirk L. Millar G. Mercadier C. Vidal (1993) ArticleTitleOvicidal and larvicidal activity of conidia and blastospores of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) against Bemisia argentifolii (Homoptera: Aleyrodidae) with a description of a bioassay system allowing prolonged survival of control insects Biocontrol Science and Technology 9 9–18

    Google Scholar 

  • C. Levis T. Giraud M. Dutertre D. Fortini Y. Brygoo (1997) ArticleTitleTelomeric DNA of Botrytis cinerea: a useful tool for strain identification FEMS Microbiology Letters 157 267–272 Occurrence Handle10.1016/S0378-1097(97)00485-0 Occurrence Handle1:CAS:528:DyaK2sXnvFGktrc%3D

    Article  CAS  Google Scholar 

  • B. Orth R. Frei P.H. Itin M.G. Rinaldi B. Speck A. Gratwohl A.F. Widmer (1996) ArticleTitleOutbreak of invasive mycoses caused by Paecilomyces lilacinus from a contaminated skin lotion Annals of Internal Medicine 125 799–806 Occurrence Handle1:STN:280:DyaK2s%2FjslOmsQ%3D%3D

    CAS  Google Scholar 

  • L.S. Osborne Z. Landa (1992) ArticleTitleBiological control of whiteflies with entomopathogenic fungi Florida Entomologist 75 456–471

    Google Scholar 

  • G. Pontecorvo J.A. Roper L.M. Hemons K.D. MacDonald A.W.J. Bufton (1953) ArticleTitleThe genetics of Aspergillus nidulans Advances in Genetics 5 141–238 Occurrence Handle1:STN:280:DyaG3s%2FmsVyrsw%3D%3D Occurrence Handle10.1016/S0065-2660(08)60408-3

    Article  CAS  Google Scholar 

  • S.O. Rogers A.J. Bendich (1988) Extraction of DNA from plant tissues S.B. Gelvin R.A. Schilperpoort D.P.S. Verma (Eds) Plant Molecular Biology Manual NumberInSeriesVol. A6 Kluwer Acadademic Publishers Dordrecht 1–10

    Google Scholar 

  • F.J. Rohlf (1993) NTSYS-pc v. 1.8. Numerical taxonomy and multivariate analysis system Applied Biostatistics Inc. Setauket, NY

    Google Scholar 

  • M.C. Rombach R.M. Aguda B.M. Shepard D.W. Roberts (1986) ArticleTitleInfection of rice brown planthopper, Nilaparvata lugens (Homoptera: Delphacidae), by field application of entomopathogenic hyphomycetes (Deuteromycotina) Environmental Entomology 15 1070–1073

    Google Scholar 

  • Samson R.A. 1974 Paecilomyces and some allied hyphomycetes. In Studies in Mycology 6 Baarn: Centraalbureau voor Schimmelcultures

  • S. Shimizu Y. Nishida H. Yoshioka T. Matsumoto (1991) ArticleTitleSeparation of chromosomal DNA molecules from Paecilomyces fumosoroseus by pulsed field electrophoresis Journal of Invertibrate Pathology 58 461–463 Occurrence Handle1:CAS:528:DyaK38Xit12jur0%3D

    CAS  Google Scholar 

  • P.A. Sneath R.R. Sokal (1973) Numerical Taxonomy Freeman San Francisco

    Google Scholar 

  • M.S. Tigano-Milani R.J. Honeycutt L. Lacey R. Assis M. McClelland B.W.S. Sobral (1995a) ArticleTitleGenetic variability of Paecilomyces fumosoroseus isolates revealed by molecular markers Journal of Invertebrate Pathology 65 274–282 Occurrence Handle1:CAS:528:DyaK2MXmtFGku7k%3D

    CAS  Google Scholar 

  • M.S. Tigano-Milani R.A. Samson I. Martins B.W.S. Sobral (1995b) ArticleTitleDNA markers for differentiating isolates of Paecilomyces lilacinus Microbiology 141 239–245 Occurrence Handle1:CAS:528:DyaK2MXjt1yjt7Y%3D Occurrence Handle10.1099/00221287-141-1-239

    Article  CAS  Google Scholar 

  • M.C. Valadares-Inglis J.F. Peberdy (1998) ArticleTitleVariation in the electrophoretic karyotype of Brazilian strains of Metarhizium anisopliae Genetics and Molecular Biology 21 1415–4757 Occurrence Handle10.1590/S1415-47571998000100003

    Article  Google Scholar 

  • M. Viaud Y. Couteaudier C. Levis G. Riba (1996) ArticleTitleGenome organization in Beauveria bassiana: electrophoretic karyotype, gene mapping and telomeric fingerprint Fungal Genetics and Biology 20 175–183 Occurrence Handle10.1006/fgbi.1996.0033 Occurrence Handle1:CAS:528:DyaK2sXivVCmtg%3D%3D

    Article  CAS  Google Scholar 

  • J.G. Williams A.R. Kubelik K.J. Livak J.A. Rafalski S.V. Tingey (1990) ArticleTitleDNA polymorphisms amplified by arbitrary primers are useful as genetic markers Nucleic Acids Research 18 6531–6535 Occurrence Handle1:CAS:528:DyaK3MXjslWmsA%3D%3D

    CAS  Google Scholar 

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Authors and Affiliations

  1. Biological Control (PCB), EMBRAPA Recursos Genéticos e Biotecnologia, C.P. 02372, CEP 70770-900, Brasília, D.F, Brazil

    Peter W. Inglis, Rubia B. C. Sarmento, Camila F. C. Gavião & M. Cléria Valadares-Inglis

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  1. Peter W. Inglis
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  2. Rubia B. C. Sarmento
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  3. Camila F. C. Gavião
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  4. M. Cléria Valadares-Inglis
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Correspondence to Peter W. Inglis.

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Inglis, P.W., Sarmento, R.B.C., Gavião, C.F.C. et al. DNA Fingerprinting of Paecilomyces Strains of Potential use for the Biological Control of Pests. World J Microbiol Biotechnol 21, 1487–1492 (2005). https://doi.org/10.1007/s11274-005-7224-3

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  • DOI: https://doi.org/10.1007/s11274-005-7224-3

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