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Decolourization of Black Oxidized Olive-Mill Wastewater by a New Tannase-Producing Aspergillus flavus Strain Isolated from Soil

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Summary

By contaminating a Tunisian soil with black oxidized and sterilized olive-mill wastewaters (OMW), 30 new indigenous fungal soil strains able to overcome the OMW toxicity could be directly selected. Ten of the fungal strains previously isolated were screened for their capability to grow in a liquid culture medium containing oxidized OMW as the only source of carbon and energy. According to these preliminary tests, strain F2 showed the best capability of removing black colour and COD (chemical oxygen demand) and was further identified as Aspergillus flavus. After optimization of batch-liquid culture conditions in the presence of oxidized OMW, the time course of biomass and enzyme production by A. flavus F2 was followed in relation to colour and COD removal. A. flavus F2 could efficiently decolourize and detoxify the black oxidized OMW (58 and 46% of colour and COD removal, respectively, after 6 days of cultivation), concomitantly with the production of tannase (8000 UI/l on day 3).

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References

  • G. Aggelis D. Iconomou M. Christou D. Bokas S. Kotzailias G. Christou V. Tsagou S. Papanikolaou (2003) ArticleTitlePhenolic removal in a model olive mill wastewater using Pleurotus ostreatus in bioreactor cultures and biological evaluation of the process Water Research 37 3897–3904 Occurrence Handle10.1016/S0043-1354(03)00313-0 Occurrence Handle1:CAS:528:DC%2BD3sXlvFejuro%3D

    Article  CAS  Google Scholar 

  • C.N. Aguilar C. Augur E. Favela-Torres G. Viniegra-Gonzalez (2001) ArticleTitleProduction of tannase by Aspergillus niger Aa-20 in submerged and solid-state fermentation: influence of glucose and tannic acid Journal of Industrial Microbiology and Biotechnology 26 296–302 Occurrence Handle10.1038/sj.jim.7000132 Occurrence Handle1:CAS:528:DC%2BD3MXlslKntbo%3D

    Article  CAS  Google Scholar 

  • American Public Health Association, 1992 Standard methods for the examination of water and wastewater. In APHA Publications 18th ed, eds. Greenberg, A.E., Clesceri, L.S. & Eaton, A.D., Washington, D.C. (USA). ISBN 0875532071

  • N. Assas L. Ayed L. Marouani M. Hamdi (2002) ArticleTitleDecolorization of fresh and stored-black olive mill wastewaters by Geotrichum candidum Process Biochemistry 38 361–365 Occurrence Handle10.1016/S0032-9592(02)00091-2 Occurrence Handle1:CAS:528:DC%2BD38XmvV2msbc%3D

    Article  CAS  Google Scholar 

  • L. Ayed M. Hamdi (2003) ArticleTitleFermentative decolorization of olive mill wastewater by Lactobacillus plantarum Process Biochemistry 39 59–65

    Google Scholar 

  • Berndt, L., Fiestas Ros de Ursinos, J.A., Geissen, K., Kachouri, M., Klimm, E., de Laborde de Monpezat, G., Xanthoulis, D. 1996 Les expériences méditerranéennes dans le traitement de l’élimination des eaux résiduaires des huileries d’olive. Projet Activité-Conseil de l’ONAS, Ministère de l’Environnement et de l’Aménagement du Territoire, Tunisia

  • T.K. Bhat H.P.S. Makkar B. Singh (1996) ArticleTitleIsolation of a tannin-protein complex-degrading fungus from faeces of hill cattle Letters in Applied Microbiology 22 257–258 Occurrence Handle1:STN:280:DyaK2s%2FovVyksQ%3D%3D

    CAS  Google Scholar 

  • T.K. Bhat B. Singh O.P. Sharma (1998) ArticleTitleMicrobial degradation of tannins-A current perspective Biodegradation 9 343–357 Occurrence Handle10.1023/A:1008397506963 Occurrence Handle1:CAS:528:DyaK1MXisVKhsbk%3D

    Article  CAS  Google Scholar 

  • R. Borja A. Martin R. Maestro J. Alba J.A. Fiestas (1992) ArticleTitleEnhancement of the anaerobic digestion of olive mill wastewater by the removal of phenolic inhibitors Process Biochemistry 27 231–237 Occurrence Handle10.1016/0032-9592(92)80023-V Occurrence Handle1:CAS:528:DyaK38XlslOmurw%3D

    Article  CAS  Google Scholar 

  • R. Capasso I. Cristinzio A. Evidente F. Scognamiglio (1992) ArticleTitleIsolation, spectroscopy and selective phytotoxic effects of polyphenols from vegetable wastewaters Phytochemistry 31 4125–4128 Occurrence Handle10.1016/0031-9422(92)80426-F Occurrence Handle1:CAS:528:DyaK3sXhs1als70%3D

    Article  CAS  Google Scholar 

  • J.A. Fiestas R. Navarro R. Leon A.J. Garcia J.M. Mastrojuan (1982) ArticleTitleDepuracion anerobia del alpechin como fuente de energia Grasas y Aceites 35 265–270

    Google Scholar 

  • O. Folin V. Ciocalteu (1927) ArticleTitleOn tyrosine and tryptophane determination in protein Journal of Biological Chemistry 73 627–650 Occurrence Handle1:CAS:528:DyaB2sXis1Cisg%3D%3D

    CAS  Google Scholar 

  • S. Goszcznski A. Paszsnski M.B. Pasti-Grigsby R.L. Crawford D.L. Crawford (1994) ArticleTitleNew pathway for degradation of sulfonated azo dyes by microbial peroxidases of Phanerochaete chrysosporium and Streptomyces chromofocus Journal of Bacteriology 176 1339–1347

    Google Scholar 

  • M. Hamdi (1993) ArticleTitleFuture prospects and constraints of olive mill wastewaters use and treatment: a review Bioprocess Engineering 8 209–214 Occurrence Handle10.1007/BF00369831 Occurrence Handle1:CAS:528:DyaK3sXksFGitbw%3D

    Article  CAS  Google Scholar 

  • M. Kissi M. Mountadar O. Assobhei E. Gargiulo G. Palmieri P. Giardina G. Sannia (2001) ArticleTitleRoles of two white-rot basidiomycete fungi in decolorization and detoxification of olive mill wastewater Applied Microbiology and Biotechnology 57 221–226 Occurrence Handle1:CAS:528:DC%2BD3MXotVSms7s%3D

    CAS  Google Scholar 

  • P. Leckha B.K. Lonsane (1997) ArticleTitleProduction and application of tannin acyl hydrolase: state of the art Advances in Applied Microbiology 44 215–260

    Google Scholar 

  • L. Martirani P. Giardina L. Marzullo G. Sannia (1996) ArticleTitleReduction of phenol content and toxicity in olive mill wastewaters with the ligninolytic fungus Pleurotus ostreatus Water Research 30 1914–1918 Occurrence Handle10.1016/0043-1354(95)00330-4 Occurrence Handle1:CAS:528:DyaK28XkvVCgt7g%3D

    Article  CAS  Google Scholar 

  • J. Perez M.T. Hernandez A. Ramos-Cormenzana J. Martinez (1987) ArticleTitleCharacterization of phenolics from olive mill wastewater and transformation by Phanerochaete chrysosporium Grasas y Aceites 38 367–371 Occurrence Handle1:CAS:528:DyaL1cXksVaqurk%3D

    CAS  Google Scholar 

  • S. Sayadi R. Ellouz (1995) ArticleTitleRoles of lignin peroxidase and manganese peroxidase from Phanerochaete chrysosporium in the decolorization of olive mill wastewaters Applied and Environmental Microbiology 61 1098–1103 Occurrence Handle1:CAS:528:DyaK2MXktV2rt78%3D

    CAS  Google Scholar 

  • S. Sharma T.K. Bhat R.K. Dawra (2000) ArticleTitleA spectrophotometric method for assay of tannase using rhodanine Analytical Biochemistry 279 85–89 Occurrence Handle10.1006/abio.1999.4405 Occurrence Handle1:CAS:528:DC%2BD3cXht1Shu70%3D

    Article  CAS  Google Scholar 

  • S. Tardoli E. Banne F. Santori (1997) ArticleTitleSpecies-specific selection on soil fungal population after olive mill waste-water treatment Chemosphere 34 2329–2336

    Google Scholar 

  • C.F. Thurston (1994) ArticleTitleThe structure and function of fungal laccases Microbiology 140 19–26 Occurrence Handle1:CAS:528:DyaK2cXkt12lsrs%3D Occurrence Handle10.1099/13500872-140-1-19

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institut National des Sciences Appliquées et de Technologie, UR – Procédés Microbiologiques et Alimentaires, Zone Urbaine Nord, BP 676, 1076, Tunis, Tunisia

    S. Kachouri, S. Halaouli & M. Hamdi

  2. IFR 86 de Biotechnologie Agro-Industrielle de Marseille, Faculté des Sciences de Luminy, UMR 1163 INRA de Biotechnologie des Champignons Filamenteux, Case 925, 13288, Marseille Cedex, 09, France

    S. Halaouli, A. Lomascolo & M. Asther

Authors
  1. S. Kachouri
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  2. S. Halaouli
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  3. A. Lomascolo
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  4. M. Asther
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  5. M. Hamdi
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Correspondence to S. Halaouli.

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Kachouri, S., Halaouli, S., Lomascolo, A. et al. Decolourization of Black Oxidized Olive-Mill Wastewater by a New Tannase-Producing Aspergillus flavus Strain Isolated from Soil. World J Microbiol Biotechnol 21, 1465–1470 (2005). https://doi.org/10.1007/s11274-005-6810-8

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  • DOI: https://doi.org/10.1007/s11274-005-6810-8

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