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Biodegradation and Decolorization of Pulp and Paper Mill Effluent by Anaerobic and Aerobic Microorganisms in a Sequential Bioreactor

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Paecilomyces sp. and Pseudomonas syringae pv myricae (CSA105) were isolated from sediment core of drainage of the pulp and paper mill industry. Fungi and bacteria were applied for treatment of pulp and paper mill effluent in a two-step and three-step fixed film sequential bioreactor containing sand and gravel at the bottom of the reactor for immobilization of microbial cells. Degradation of chlorinated phenols and formation of their metabolites were determined by high performance liquid chromatography. The microbes exhibited significant reduction in colour (88.5%), lignin (79.5%), chemical oxygen demand (87.2%) and phenol (87.7%) in two-step aerobic sequential bioreactor, and colour (87.7%), lignin (76.5%), chemical oxygen demand (83.9%) and phenol (87.2%) in three-step anaerobic-aerobic sequential bioreactor.

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  • A. Abassi (1985) ArticleTitleOccurrence, toxicity and treatment of lignin in pulp and paper effluents. State of art IE(I) Journal of Environment 65 1–8

    Google Scholar 

  • M. Alexander (1981) ArticleTitleBiodegradation: Problems of molecular recalcitrance and microbial fallibility Advances in Applied Microbiology 7 35–80

    Google Scholar 

  • J.H. Apajalathi P. Karpahoja M.S. Salkinoja (1986) ArticleTitleDegradation of polychlorinated phenols by Rhodococcus chlorophenolicus Applied Microbiology and Biotechnology 25 62–67

    Google Scholar 

  • APHA, 1995 Standard methods for the examination of water and waste water. 19th ed. Washington, DC: American Public Health Association. ISBN 0875532233.

  • Bajpai P.K., 1999 Proc. of Interaction meeting on Biotechnological Application in the Pulp and Paper Industry. Central Pulp and Paper Research Institute, Saharanpur. 1999. pp. 411.

  • P. Bajpai P.K. Bajpai (1997) ArticleTitleReduction of organochlorine compounds in bleach plant effluent Advances in Biochemical Engineering and Biotechnology 57 213–259 Occurrence Handle1:CAS:528:DyaK2sXktlCktLo%3D

    CAS  Google Scholar 

  • P. Bajpai P. K. Bajpai (1994) ArticleTitleMini Review: Biological colour removal of pulp and paper mill wastewater Journal of Biotechnology 33 211–220 Occurrence Handle10.1016/0168-1656(94)90069-8 Occurrence Handle1:CAS:528:DyaK2cXislyrtLY%3D

    Article  CAS  Google Scholar 

  • P. Bajpai A. Mehna P.K. Bajpai (1993) ArticleTitleDecolorization of Kraft Bleach Plant Effluent with the white rot fungus Trametes versicolor Process Biochemistry 28 377–384 Occurrence Handle10.1016/0032-9592(93)80024-B Occurrence Handle1:CAS:528:DyaK3sXlsVGktrg%3D

    Article  CAS  Google Scholar 

  • M.F. da Homa A.I. Haddad M.F. Abd-El-Bary (1987) ArticleTitleTreatment of phenolic wastes in an aerated submerged fixed-film (ASFF) bioreactor Journal of Biotechnology 5 279–292

    Google Scholar 

  • K.E. Eriksson K.C. Kolar (1985) ArticleTitleMicrobial degradation of chlorolignins Environmental Science and Technology 19 1086–1089 Occurrence Handle1:CAS:528:DyaL2MXlsVemurg%3D

    CAS  Google Scholar 

  • M.M. Haggblom M.S. Salonen (1992) ArticleTitleBiodegradability of chlorinated organic compounds in pulp bleaching effluents Water Science and Technology 24 161–170

    Google Scholar 

  • R.F. Hickey W.W. Jones M.C. Veiga (1995) ArticleTitleThe start-up operation, monitoring and control of high-rate anaerobic treatment system Water Science and Technology 24 207–255

    Google Scholar 

  • D.J. Mc-leay (1979) ArticleTitleInfluence of dilution water on the toxicity of kraft pulp and paper mill effluent including mechanism of effect Water Research 13 221–236

    Google Scholar 

  • Palleroni, N.J., 1984 Pseudomonas. In Bergey’s Manual of Systematic Bacteriology, eds, Krieg, N.R. & Holt, J.G. pp 141–198. Baltimore: Williams & Wilkins. ISBN 0683041088.

  • I.A. Pearl H.K. Benson (1940) ArticleTitleThe determination of lignin in sulphide pulping liquor Paper Trade Journal 111 35 Occurrence Handle1:CAS:528:DyaH3MXhvVSn

    CAS  Google Scholar 

  • P. Radehans S. K. Schmidt (1992) ArticleTitleCharacterization of noval Pseudomonas sp. that mineralizes high concentration of PCP Applied and Environmental Microbiology 58 2879–2885

    Google Scholar 

  • K. Raju S. Ramalinghaih (1997) ArticleTitleMethane production from orange waste Indian Journal of Environmental Health 30 20–22

    Google Scholar 

  • I.S. Thakur (1995) ArticleTitleStructural and functional characterization of a stable, 4-cholrosalicylic-acid-degrading bacteria community in a chemostat World Journal of Microbiology and Biotechnology 11 643–645 Occurrence Handle10.1007/BF00361007

    Article  Google Scholar 

  • I.S. Thakur P. Verma K. Upadhyaya (2001) ArticleTitleInvolvement of plasmid in degradation of pentachlorophenol by Pseudomonas sp. from a chemostat Biochemical and Biophysics Research Communications 286 109–113 Occurrence Handle1:STN:280:DC%2BD3MvjvFyhtg%3D%3D

    CAS  Google Scholar 

  • K.A. Vora C. Singh V.V. Modi (1988) ArticleTitleDegradation of 2-fluorobenzoate by a Pseudomonad Current Microbiology 17 249–254 Occurrence Handle10.1007/BF01571323 Occurrence Handle1:CAS:528:DyaL1cXlvVKqur8%3D

    Article  CAS  Google Scholar 

  • M. Wieser J. Eberspacher B. Vogler F. Lingens (1994) ArticleTitleMetabolism of 4-chlorophenol by Azotobacter sp. GP1: Structure of the meta cleavage product of 4-chlorocatechol FEMS Microbiology Letters 116 73–78 Occurrence Handle10.1016/0378-1097(94)90110-4 Occurrence Handle1:CAS:528:DyaK2cXhvVKqu7o%3D

    Article  CAS  Google Scholar 

  • P. Yu T. Welander (1994) ArticleTitleAnaerobic treatment of kraft bleaching plant effluent Applied and Microbiology and Biotechnology 40 806–811 Occurrence Handle1:CAS:528:DyaK2cXksVWrur4%3D

    CAS  Google Scholar 

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Correspondence to Indu Shekhar Thakur.

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Chuphal, Y., Kumar, V. & Thakur, I.S. Biodegradation and Decolorization of Pulp and Paper Mill Effluent by Anaerobic and Aerobic Microorganisms in a Sequential Bioreactor. World J Microbiol Biotechnol 21, 1439–1445 (2005).

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