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Optimization of Process Parameters for the Production of Inulinase from a Newly Isolated Aspergillus niger AUP19

Summary

Fifty strains were isolated from different soil samples on synthetic medium containing inulin as a sole carbon source for the production of extracellular inulinase. Of them, five isolates showed high inulinase activity and one of them was selected for identification and medium optimization studies. The isolate was identified as Aspergillus niger. Various physical and chemical parameters were optimized for inulinase production. Maximum productivity of inulinase (176 U ml−1) was achieved by employing medium containing 5% (w/v) inulin, galactose as additional carbon source, corn steep liquor and (NH4)H2PO4 as nitrogen sources, incubation period of 72 h, incubation temperature of 28 °C, pH 6.5, inoculum load at 10% (v/v) level and medium volume to flask volume ratio of 1:20 (v/v) with indented flasks.

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References

  • K. Anuradha K. Narinder K.G. Anil (1997) ArticleTitleInulinase form Aspergillus versicolor: a potent enzyme for producing fructose from inulin Journal of Scientific and Industrial Research 56 721–726

    Google Scholar 

  • R.A. Burne J.E.C. Penders (1992) ArticleTitleCharacterization of the Streptococcus mutants GS-5 fruA gene encoding exo-B-D-fructosidase Infection and Immunity 60 4621–4632 Occurrence Handle1:CAS:528:DyaK3sXkvV2qsr8%3D

    CAS  Google Scholar 

  • R.A. Burne K. Shilling W.H. Bowen R.E. Yasbin (1987) ArticleTitleExpression, purification and characterization of an exo-B-D-fructosidase of Streptococcus mutants Journal of Bacteriology 169 4507–4517 Occurrence Handle1:CAS:528:DyaL2sXmtlWqsbk%3D

    CAS  Google Scholar 

  • M.L. Cazetta P.M.M. Martins R. Monti J. Contiero (2005) ArticleTitleYacon (Polymnia sanchifolia) extract as a substrate to produce inulinase by Kluyveromyces marxianus var. bulgaris Journal of Food Engineering 66 301–305 Occurrence Handle10.1016/j.jfoodeng.2004.03.022

    Article  Google Scholar 

  • D.G. Derycke E.J Vandamme (1984) ArticleTitleProduction and properties of Aspergillus niger Inulinase Journal of Chemical Technology and Biotechnology 34 45–51 Occurrence Handle1:CAS:528:DyaL2cXksFehtb0%3D

    CAS  Google Scholar 

  • I. Efstathiou G. Reysset N. Truffaut (1986) ArticleTitleA study of inulinase activity in the Clostridium acetobutylicum strain ABKn8 Applied Microbiology and Biotechnology 25 143–149 Occurrence Handle1:CAS:528:DyaL2sXhslWlurc%3D

    CAS  Google Scholar 

  • A.K. Gupta P. Rathore N. Kaur R. Singh (1990) ArticleTitleProduction, thermal stability and immobilization of Inulinase from Fusarium oxysporum Journal of Chemical Technology and Biotechnology 47 245–257 Occurrence Handle1:CAS:528:DyaK3cXhsFKltr8%3D

    CAS  Google Scholar 

  • C.G. Joseph (1967) A Manual of Soil Fungi EditionNumberIndian Ist edn Oxford, IBH Publishing Co New Delhi 228

    Google Scholar 

  • H.F. Kuehnle K. Dahl ParticleVon F.H. Schmidt (1992) ArticleTitleFully enzymic inluin determination in small volume samples without deproteinization Nephron 62 104–107 Occurrence Handle1:CAS:528:DyaK38Xlt1Omsrg%3D Occurrence Handle10.1159/000187006

    CAS  Article  Google Scholar 

  • F. Kunst M. Steinmetz J.A. Lepesant R. Dedonder (1977) ArticleTitlePresence of a third sucrose hydrolyzing in Bacillus subtilis: constitutive levanase synthesis of mutants of Bacillus subtilis Marburg 168 Biochimie 59 287–292 Occurrence Handle1:CAS:528:DyaE2sXktFylsr0%3D

    CAS  Google Scholar 

  • O. Laloux J.P. Cassart J. Delcour J. Beeumen ParticleVan J. Vandenhaute (1991) ArticleTitleCloning and sequencing of the inulinase gene of Kluyveromyces marxianus var. marxianus ATCC 12424 FEBS Letters 289 64–68 Occurrence Handle10.1016/0014-5793(91)80909-M Occurrence Handle1:CAS:528:DyaK38Xlt1Gms7c%3D

    Article  CAS  Google Scholar 

  • P. Looten D. Blanchet J.P. Vandecasteele (1987) ArticleTitleThe β-fructofuranosidase activities of a strain of Clostridium acetobutylicum grown on inulin Applied Microbiology and Biotechnology 25 419–425 Occurrence Handle10.1007/BF00253311 Occurrence Handle1:CAS:528:DyaL2sXktVejsLc%3D

    Article  CAS  Google Scholar 

  • G.L. Miller (1959) ArticleTitleUse of dinitrosalicylic acid reagent for determination of reducing sugars Analytical Chemistry 31 426–428 Occurrence Handle1:CAS:528:DyaG1MXmtFKiuw%3D%3D

    CAS  Google Scholar 

  • R.J. Rouwenhorst W.S. Ritmeester W.A. Scheffers J.P. Dijken ParticleVan (1990) ArticleTitleLocalization of inulinase and invertase in Kluyveromyces species Applied and Environmental Microbiology 56 3329–3336 Occurrence Handle1:CAS:528:DyaK3MXjt1an

    CAS  Google Scholar 

  • P. Selvakumar A. Pandey (1998) ArticleTitleIsolation and characterization of inulinase producing strains from Rhizosphere soil Journal of Scientific and Industrial Research 57 621–624 Occurrence Handle1:CAS:528:DyaK1cXnvVWkurk%3D

    CAS  Google Scholar 

  • E.J. Vandamme D.G. Derycke (1983) ArticleTitleMicrobial inulinases: fermentation process, properties and applications Advances in Applied Microbiology 29 139–176 Occurrence Handle1:CAS:528:DyaL2cXlt1ykt7s%3D Occurrence Handle10.1016/S0065-2164(08)70356-3

    CAS  Article  Google Scholar 

  • P. Viswanathan P.R. Kulakarni (1996) ArticleTitleInulinase producing fungi and actinomycetes from Dahlia rhizosphere Indian Journal of Microbiology 36 117–118

    Google Scholar 

  • D.L. Vullo C.E. Coto F. Sineriz (1991) ArticleTitleCharacteristics of an inulinase produced by Bacillus subtilis 30A, a strain isolated from the rhizosphere of Vernonia herbacia (Vell Rusby) Applied and Environmental Microbiology 57 499–508

    Google Scholar 

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Correspondence to A. Kunamneni.

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Kumar, G.P., Kunamneni, A., Prabhakar, T. et al. Optimization of Process Parameters for the Production of Inulinase from a Newly Isolated Aspergillus niger AUP19. World J Microbiol Biotechnol 21, 1359–1361 (2005). https://doi.org/10.1007/s11274-005-5078-3

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  • DOI: https://doi.org/10.1007/s11274-005-5078-3

Keywords

  • Aspergillus niger
  • fungi
  • inulinase production
  • optimization
  • screening