The ability of Streptomyces sp. OXCI, S. rimosus NRRL B2659, S. rimosus NRRL B2234, S. alboflavus NRRL B1273 S. aureofaciens NRRL B2183 and S. vendagensis ATCC 25507 to produce tetracycline using some local agricultural wastes as solid state media, were assessed. The wastes employed include peanut (groundnut) shells, corncob, corn pomace and cassava peels. Bacillus subtilis ATCC 6633 was used to assay antimicrobial activity. All the strains produced tetracycline in a solid-state fermentation process containing peanut (groundnut) as the carbohydrate source. Streptomyces sp. OXC1 had the highest ability for tetracycline production with peanut shells as the substrate in solid fermentation (13.18 mg/g), followed by S. vendagensis ATCC 25507 (11.08 mg/g), S.␣rimosus NRRL B1679 (8.46 mg/g), S. alboflavus NRRL B1273 (7.59 mg/g), S. rimosus NRRL B2234 (6.37 mg/g), S. aureofaciens NRRL B2183 (4.27 mg/g). Peanut (groundnut) shells were the most effective substrate (4.36 mg/g) followed by corncob (2.64 mg/g), cassava peels (2.16 mg/g) and corn pomace (1.99 mg/g). The composition of the peanut (groundnut) shell medium optimal for tetracycline production were peanut shells 100 g, organic nitrogen (peanut meal) 10 g, (NH 4)2 SO4 1 g, KH2 PO 4 0.5 g, CaCO3 > 0.5 g, NaCl 0.5 g, MgSO4 · 7H2 O 0.5 g, soluble starch 10 g, peanut oil 0.25 ml with initial moisture content of 65–68%, and initial pH 5.3–6.3. Substrate (1 g dry weight) was inoculated with 1.0 × 10 8 conidia per ml and incubated at 28–31 °C for 5–7 days, producing 13.18 mg/g of total tetracycline. Tetracycline detection started on day 3 and attained its maximum level on day 5.
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L. Ahmed P. Germain G. Lefebvre (1987) ArticleTitlePhosphate repression of cephamycin and clavulanic acid production by Streptomyces clavuligerus Applied Microbiology and Biotechnology 26 130–135
L.D. Baver (1956) Soil Physics EditionNumber3 Wiley New York 178
R.K. Bhatnagar J.L. Doull L.C. Vining (1988) ArticleTitleRole of the carbon source in regulating chloramphenicol production by Streptomyces venezuelae studies in batch and continuous cultures Canadian Journal of Microbiology 34 1217–1233
J.H. Huang (1972) Antibiotics Tafu Taipei 234–268
Ifudu, N.D. 1986a Indigenous resources for antibiotic production. pp. 27–32. Aug/Sept edition. Expansion Today (Nigeria). Nigeria: AU Press Ltd.
Ifudu, N.D. 1986b Indigenous resources for antibiotic production pp. 52–53. Nov/Dec edition. Expansion Today (Nigeria). Nigeria: AU Press Ltd.
K.I. Komatsu M. Mizuno R Kodaira (1975) ArticleTitleEffect of methionines on cephalosporin C and penicillin N production by a mutant of Cephalosporium acremonium Journal of Antibiotics 28 881–888
Y. Okami O. Oomura (1979) Production of Antibiotic Substances Kyoritsu Press Ltd Tokyo
M.J. Pelczar E.C.S. Chan N.R. Krieg (1993) Microbiology Concepts and Applications. McGraw-Hill Inc. NY
A. Rake (1998) New African Year Book EditionNumber12 I.C Publication. London, UK
Y.Q. Shen J. Heim N.A. Solomon A.L Wolfe S. Demain (1984) ArticleTitleRepression of beta-lactam production in Cephalosporium acremonium by nitrogen sources Journal of Antibiotics 37 503–511
Somerson, N.L. & Phillips, T. 1961 Production of glutamic acid. U.S Patent 3,089,297, 37-1, 695.
D.H. Treskatis R. King H. Wolf E.D. Galles (1992) ArticleTitleNutritional Control of nikkomycin and juglomycin production by Streptomyces tendae in continuous culture Applied Microbiology and Biotechnology 36 440–445
Wang, H.H. 1989. Utilization of particulate agricultural products through solid state fermentation. Proceedings of the National Science Council, Republic of China, Part B,13, 1455–159.
D.M. Ward R. Weller M.M. Bateson (1990) ArticleTitle16SrRNA sequence reveal numerous uncultured microorganisms in a natural community Nature 345 63–65 Occurrence Handle10.1038/345063a0 Occurrence Handle1:CAS:528:DyaK3cXktlOltbo%3D Occurrence Handle1691827
M.G. Watve V. Shejval C. Sonawane M. Rahalkar M. Matapurkar Y. Shouche M. Patole N. Phadnis A. Champhekar K. Damle S. Karandikar V. Kshiragar M. Jog (2000) ArticleTitleThe ‘K’ selected oligiophilic bacteria: a key to uncultured diversity Current Science 78 1535–1542
M.G. Watve R. Tickoo M.M. Jog B.D. Bhole ( 2001) ArticleTitleHow many antibiotics are produced by the genus Streptomyces Archives of Microbiology 177 86–90
S.S. Yang (1977) ArticleTitleQuantitative determination of soil gas with regard to soil microbial activities National Science Council Monthly 5 478–502
S.S. Yang (1988) ArticleTitleProtein enrichment of sweet potato residue with amylolytic yeasts by solid state fermentation Biotechnology and Bioengineering 32 886–890
S.S. Yang (1993) ArticleTitleProtein enrichment of sweet potato residue with co-culture of amylolytic fungi by solid state fermentation Biotechnology Advances 11 495–505
S.S. Yang Z.J. Cheng (1991) ArticleTitleProtein enrichment of corncob with Trichoderma by solid-state fermentation Chinese Journal of Microbiology and Immunology 24 177–195
S.S. Yang C.I. Huang (1994) ArticleTitleProtease production by amylolytic fungi in solid state germentation Biotechnology and Bioengineering 32 886–890
S.S. Yang M.Y. Ling (1989) ArticleTitleTetracycline production with sweet potato residues by solid state fermentation Biotechnology and Bioengineering 33 1021–1028
S.S. Yang W.J. Swei (1996) ArticleTitleCultural condition and oxytetracycline production by Streptomyces rimosus in solid state fermentation of corncob World Journal of Microbiology and Biotechnology 12 43–46
S.S. Yang S.S. Yuan (1990) ArticleTitleOxytetracycline production by Streptomyces rimosus in solid state fermentation of sweet potato residue World Journal of Microbiology and Biotechnology 6 236–244
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Asagbra, A.E., Sanni, A.I. & Oyewole, O.B. Solid-state fermentation production of tetracycline by Streptomyces strains using some agricultural wastes as substrate. World J Microbiol Biotechnol 21, 107–114 (2005). https://doi.org/10.1007/s11274-004-2778-z
- peanut shells
- solid-state fermentation